FastQCFastQC Report
Fri 17 Jun 2016
SRR1526938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42480
Sequences flagged as poor quality0
Sequence length64
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.6803201506591338No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.31779661016949157No Hit
GACACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.29896421845574384No Hit
GACACTGGGGCAGCATTGGACATTAATACACAGGGGAAATGTCCAGAAAAGCACATGAAAAGGA1060.2495291902071563No Hit
GACACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA760.17890772128060262No Hit
GACACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.1694915254237288No Hit
GACACTTGGGTGAAATTTCTTTGCTGATTTTTTGGTCAGAATTATCTATCTATTGATGAGAGTT720.1694915254237288No Hit
GACACTGGGAACATTTTGGTTTTTCATCCTGGACATGATGCGCATTGTAAATTGCATTAGAATT690.16242937853107345No Hit
GACACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.1483050847457627No Hit
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT610.1435969868173258No Hit
GACACTGGGATCTAATTCCACTTATAGGCCTATTAATCGCAGCTACAGGAAAATCAGCACAATT540.1271186440677966No Hit
GACACTGGGGTTTCTGATGTCTGCAATCACCTTTAATTCAGCCTGTTCTACTTCCCACCCTTCT510.12005649717514125No Hit
GACACTGGGGCTACCCCTGGTTCTGCTTTCATGCTGATAATGTACTGAGACTTCGGTATTTGAC490.11534839924670433No Hit
GACACTGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA460.10828625235404896No Hit
GACACTGGGATTTGCTACATAATAGACATTGTGCCAAATAATTCCTTTTTTATTTATTTTATTC450.1059322033898305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAT150.00450485158.48510457
ATTAGAA150.00450485158.48510456
GGGTTAC150.004569076558.2767267
TTGGGTG150.004569076558.2767266
GGGATGA150.004569076558.2767267
TGGGCAC303.5558878E-758.2767266
ACTAGGA150.004569076558.2767264
CTGGGTC452.910383E-1158.2767225
GGGTACG258.2888655E-658.2767227
GGGATAT201.9418777E-458.2767227
TGGGTAT201.9418777E-458.2767226
GGATATC150.004612270758.1386268
GGTACGC150.004612270758.1386268
GGTACCT201.9648392E-458.1386268
TGATGCG150.004655768658.00118636
TGATGAG150.004655768658.00118654
TGCATTA150.004655768658.00118653
TGCGCAT150.004655768658.00118639
ATGATGC150.004655768658.00118635
TTATCTC150.004655768658.00118647
Produced by FastQC (version 0.11.5)