FastQCFastQC Report
Fri 17 Jun 2016
SRR1526937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56373
Sequences flagged as poor quality0
Sequence length64
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4260.7556809110744505No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.7521331133698756No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2080.36897096127578805No Hit
GACACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC2060.3654231635712132No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.3476841750483387No Hit
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1940.34413637734376384No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1890.33526688308232666No Hit
GACACTGGGGCAGCATTGGACATTAATACACAGGGGAAATGTCCAGAAAAGCACATGAAAAGGA1880.3334929842300392No Hit
GACACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.20577226686534336No Hit
GACACTGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC1080.1915810760470438No Hit
GACACTGGGATGGCAAGGTTTGCGGATTTTATCCGGGGCATGCTGAAACTGATCCTGCTCCTCC1040.18448548063789402No Hit
GACACTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1040.18448548063789402No Hit
GACACTGGGACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCA900.15965089670586982No Hit
GACACTTGGGTGAAATTTCTTTGCTGATTTTTTGGTCAGAATTATCTATCTATTGATGAGAGTT890.1578769978535824No Hit
GACACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG890.1578769978535824No Hit
GACACTGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATA840.14900750359214518No Hit
GACACTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG790.14013800933070794No Hit
GACACTGGGCATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGAC780.13836411047842054No Hit
GACACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA780.13836411047842054No Hit
GACACTGGGGGATCATCACTAACAACAGCAACAGGAATAAAAAGTACAACCATGGCACCCTGAG770.13659021162613308No Hit
GACACTGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAACCTAAA760.13481631277384565No Hit
GACACTGGGATCTAATTCCACTTATAGGCCTATTAATCGCAGCTACAGGAAAATCAGCACAATT740.13126851506927076No Hit
GACACTGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA740.13126851506927076No Hit
GACACTGGGGCTACCCCTGGTTCTGCTTTCATGCTGATAATGTACTGAGACTTCGGTATTTGAC730.1294946162169833No Hit
GACACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.12772071736469587No Hit
GACACTGGGAACATTTTGGTTTTTCATCCTGGACATGATGCGCATTGTAAATTGCATTAGAATT720.12772071736469587No Hit
GACACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.12417291966012098No Hit
GACACTGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG700.12417291966012098No Hit
GACACTGGGGCTGACACTGGGGGACCAGTGTGAGAGTTCAACCAGGAGGCATCAGGAGGCTCAG680.1206251219555461No Hit
GACACTGGGGACGCCTGAGATAAATACCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGAC640.11352952654639632No Hit
GACACTGGGACAGTGAGCCTGCGGGGAGGTGGGAGAGCCGGGAAAGTCTGGTATCCCCTGCCCC630.11175562769410888No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT620.10998172884182143No Hit
GACACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC590.10466003228495913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAT150.00457498158.282957
TATGATA150.00457498158.282958
TAACCTA150.00457498158.282956
CAATAAT201.9463993E-458.282956
TCTATGA150.00457498158.282956
TCACAAT201.9637177E-458.17873441
CAATTAT201.9637177E-458.17873444
TTATCTC201.9637177E-458.17873447
TGATTTA201.9637177E-458.17873434
ACAATTA201.9637177E-458.17873443
TAGAGCA150.004607540658.1787335
GTCGCCA150.004607540658.1787346
ATAATTC150.004607540658.1787338
CATTGAG150.004607540658.1787335
ACATTGA150.004607540658.1787334
GCAAACT150.004607540658.1787337
GTTAGAG150.004607540658.1787333
TTATCTA150.004607540658.1787342
CTGAAAC150.004607540658.1787343
ATTCCTT150.004607540658.1787341
Produced by FastQC (version 0.11.5)