FastQCFastQC Report
Fri 17 Jun 2016
SRR1526936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55579
Sequences flagged as poor quality0
Sequence length64
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.678313751596826No Hit
GACACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG3660.6585221036722503No Hit
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2030.3652458662444449No Hit
GACACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC1870.3364580147177891No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1840.3310602925565411No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1810.32566257039529317No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.30407168175030136No Hit
GACACTGGGGCAGCATTGGACATTAATACACAGGGGAAATGTCCAGAAAAGCACATGAAAAGGA1670.30047320030946945No Hit
GACACTGGGATGGCAAGGTTTGCGGATTTTATCCGGGGCATGCTGAAACTGATCCTGCTCCTCC1100.19791647924575828No Hit
GACACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.18892027564367836No Hit
GACACTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC980.17632559060076647No Hit
GACACTGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC960.1727271091599345No Hit
GACACTGGGAACATTTTGGTTTTTCATCCTGGACATGATGCGCATTGTAAATTGCATTAGAATT920.16553014627827056No Hit
GACACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA890.16013242411702264No Hit
GACACTGGGATCTAATTCCACTTATAGGCCTATTAATCGCAGCTACAGGAAAATCAGCACAATT870.15653394267619064No Hit
GACACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG860.15473470195577466No Hit
GACACTGGGCATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGAC830.14933697979452673No Hit
GACACTTGGGTGAAATTTCTTTGCTGATTTTTTGGTCAGAATTATCTATCTATTGATGAGAGTT830.14933697979452673No Hit
GACACTGGGACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCA710.1277460911495349No Hit
GACACTGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAACCTAAA700.1259468504291189No Hit
GACACTGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG690.12414760970870292No Hit
GACACTGGGGGATCATCACTAACAACAGCAACAGGAATAAAAAGTACAACCATGGCACCCTGAG690.12414760970870292No Hit
GACACTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG690.12414760970870292No Hit
GACACTGGGGCTACCCCTGGTTCTGCTTTCATGCTGATAATGTACTGAGACTTCGGTATTTGAC680.12234836898828694No Hit
GACACTGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATA640.115151406106623No Hit
GACACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.11335216538620702No Hit
GACACTGGGGACGCCTGAGATAAATACCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGAC610.10975368394537505No Hit
GACACTGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA580.1043559617841271No Hit
GACACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC560.10075748034329512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC201.9225512E-458.4267522
CCCTCAT150.004530159358.4267520
AAATACC150.004530159358.4267522
TAGACAT150.004530159358.4267523
GACGTGT150.004530159358.4267523
TAGAAAC201.9225512E-458.4267523
ATAGACA150.004530159358.4267522
GCACACA201.9225512E-458.4267523
CGTAGAA150.004530159358.4267521
ATGACGT150.004530159358.4267521
AAGGGTC150.004530159358.4267526
AATAGAC150.004530159358.4267521
AATACCG150.004530159358.4267523
ATACCGC150.004530159358.4267524
TACCGCA150.004530159358.4267525
TTCAGTG150.00454652358.37363445
CCAACCT150.00454652358.37363445
TCGCCCG150.00456293158.32061839
AAAAGTA150.00456293158.32061840
AAAGTAC150.00456293158.32061841
Produced by FastQC (version 0.11.5)