FastQCFastQC Report
Fri 17 Jun 2016
SRR1526935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49529
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4380.8843303922954229No Hit
GACACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA3210.6481051505178784No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2240.45226029195017065No Hit
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2110.4260130428637768No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.40784187041935027No Hit
GACACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG1870.3775565830119728No Hit
GACACTGGGGCAGCATTGGACATTAATACACAGGGGAAATGTCCAGAAAAGCACAT1230.24833935674049545No Hit
GACACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.24228229925901995No Hit
GACACTGGGGGATCATCACTAACAACAGCAACAGGAATAAAAAGTACAACCATGGC940.1897878010862323No Hit
GACACTGGGAACATTTTGGTTTTTCATCCTGGACATGATGCGCATTGTAAATTGCA900.18171172444426498No Hit
GACACTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC890.17969270528377315No Hit
GACACTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC830.16757859032082215No Hit
GACACTGGGATGGCAAGGTTTGCGGATTTTATCCGGGGCATGCTGAAACTGATCCT810.16354055199983847No Hit
GACACTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA800.16152153283934664No Hit
GACACTGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC750.1514264370368875No Hit
GACACTGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCT730.1473883987159038No Hit
GACACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.145369379555412No Hit
GACACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC710.14335036039492013No Hit
GACACTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG690.13931232207393648No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC680.13729330291344463No Hit
GACACTTGGGTGAAATTTCTTTGCTGATTTTTTGGTCAGAATTATCTATCTATTGA660.13325526459246098No Hit
GACACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC650.13123624543196916No Hit
GACACTGGGATCTAATTCCACTTATAGGCCTATTAATCGCAGCTACAGGAAAATCA620.12517918795049365No Hit
GACACTGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACT610.12316016879000181No Hit
GACACTGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCAT600.12114114962950998No Hit
GACACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG600.12114114962950998No Hit
GACACTGGGGACGCCTGAGATAAATACCGCAAAGCAGGAGCTGGGGACTAGACCGC590.11912213046901814No Hit
GACACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10700801550606714No Hit
GACACTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG520.10498899634557532No Hit
GACACTGGGGGTTCAGCCGGGATCGATGTTTGCTGGCATCGCCTCGCCCTGTCTTG520.10498899634557532No Hit
GACACTGGGGCTACCCCTGGTTCTGCTTTCATGCTGATAATGTACTGAGACTTCGG510.10296997718508348No Hit
GACACTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC510.10296997718508348No Hit
GACACTGGGGGTTTCTAGGTAGCCAAGGATGGCCTTTGAATTTCTGATCCTCCTGT510.10296997718508348No Hit
GACACTGGGGGCTTGTGTTTGCCAGCAGCTTGCGGCACCGGGAATGATGGACAGCC500.10095095802459164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTGC150.00804099950.52971650
CAAGGTG150.00804099950.52971649
TGAGTGG150.00804099950.52971649
CTGAGTG150.00807366450.47799748
TTAGAAA150.00827172850.1698945
GTGTCTT150.00827172850.1698937
GAAATGT150.00827172850.1698936
ATGCGCA150.00827172850.1698938
AGAAAAG150.00827172850.1698945
GTCCAGA150.00827172850.1698941
GTGACCT150.00827172850.1698939
AAATGTC150.00827172850.1698937
TGGCCAA150.00827172850.1698945
TAGCAAG550.050.16988847
TGCCTAG550.050.16988843
CTAGCAA550.050.16988846
CCTAGCA550.050.16988845
TTGCCTA507.2759576E-1250.16988842
CATGAGT150.00830508750.11890435
AGGGCGC150.00830508750.11890435