FastQCFastQC Report
Fri 17 Jun 2016
SRR1526934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458412
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC19610.4277811226582201No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC12440.27137160458277704No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC7480.16317199375234506No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC6470.14113941170824498No Hit
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.12652373847106968No Hit
TGGATGGGAGTTTTGTAGACTGTACAGAAATTGGCACCCTATTTTCTTGCAGCTCTCAGATTTT4870.10623631144036369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG150.004679129558.019742
TTACGTT150.00471778157.8992813
CATGATA150.004725948457.8739925
CCGTAAA303.8337384E-757.82978420
ATGGGGG262350.056.8441854
GGATGGG455100.056.8439372
GATGGGG436250.056.774583
TGGATGG461300.056.738051
ATGGGGA115900.056.0086444
ATGGGTA1100.055.497884
ATGGGGC34350.055.347854
ATGGGGT26550.055.0748334
TGGGGGG149000.054.940955
TGGGGGA52950.054.5720375
CGACTAT600.053.19637344
TGGGGGC44300.052.365945
TGGGGAG45800.051.9838185
TGGGGGT29200.051.9681665
ACGACAC452.068191E-951.47727630
TGGGGAC21500.050.704045