FastQCFastQC Report
Fri 17 Jun 2016
SRR1526932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622949
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC53250.8548051285097175No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC37160.5965175319327907No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC28860.46328030063456244No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC19240.3088535337563749No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC14600.23436910565712443No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT14470.23228225745606781No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC14050.22554013249880808No Hit
TGGATGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG12870.20659797190460213No Hit
TGGATGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG11320.18171632027661977No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC8800.1412635705330613No Hit
TGGATGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG8210.13179249023595832No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT7990.1282609009726318No Hit
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.1253714188480919No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC7740.12424773135521529No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC7250.11638191890507892No Hit
TGGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG6860.11012137430190916No Hit
TGGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC6580.10562662433040267No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCT6540.10498451719161601No Hit
TGGATGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT6410.10289766899055942No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT6390.1025766154211661No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT6310.10129240114359281No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6260.10048976722010952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGTC150.004546759758.44423743
TACGTAC201.9383657E-458.4252440
AAACTCG150.004664959458.06662856
CAATCGG150.004724910557.8796531
ATCGTTA150.00472792357.8703329
TATCGTT150.004736969257.84239628
TCTAGCG150.00477935557.7123913
GATTCGT150.00477935557.712399
ATGGGGG366950.056.7327544
GATGGGG595750.056.505623
TGGATGG628950.056.4368131
GGATGGG622150.056.360642
ATGGGGA147400.055.6981544
TGGGGGG195450.055.341535
ATGGGGT32600.054.7820054
TGGGGGA74700.054.7784425
ATGGGGC52400.054.3766824
TGGGGGT39800.054.0928965
TGGGGGC69800.053.82145
TGGGGAC27900.053.134945
Produced by FastQC (version 0.11.5)