FastQCFastQC Report
Fri 17 Jun 2016
SRR1526931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567739
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC103371.8207310049159913No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC42760.7531629851040708No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC17370.30595044553923545No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC15960.2811150898564305No Hit
TGGATGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC12920.22756935845520565No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT11070.19498396270117077No Hit
TGGATGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT10880.19163735448859423No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT9720.17120543066444263No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC8960.1578189978141364No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC8790.15482466415025214No Hit
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8630.152006467760714No Hit
TGGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8230.14496097678686862No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGCTCTTCC7740.13633025034390803No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCC7310.12875634754702425No Hit
TGGATGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6680.11765969926321779No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC6680.11765969926321779No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCA6600.11625060106844871No Hit
TGGATGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6420.1130801301302183No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC6240.10990965919198788No Hit
TGGATGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC6240.10990965919198788No Hit
TGGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5770.10163120729771956No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA5760.10145507002337342No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA5720.10075052092598888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTCG150.00800519450.6564848
TAATCGT150.00829840150.197643
CGATAAG252.0660023E-550.0819633
GCCGATA204.1665818E-450.0376231
GATACGG252.1407393E-549.78201313
GTATTAC150.00857561649.78201311
CGCAAAC150.00857561649.78201314
TGGATGG571750.048.8851971
GGATGGG565950.048.783342
GATGGGG545200.048.7768023
ATGGGGG334250.048.7672354
ATGGGGA137400.048.291174
ATGGGGC47050.047.9959834
TGGGGGG177550.047.8740435
TGGGGGA65600.047.7456745
ATGGGGT29400.047.593344
TGGGGGC63250.046.9216165
TGGGGGT36250.046.9104845
TGGGGAC24150.046.1868255
TGGGGAG54800.045.5244185
Produced by FastQC (version 0.11.5)