FastQCFastQC Report
Fri 17 Jun 2016
SRR1526930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30217
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.5592878181156302No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1280.4236026078035543No Hit
TGTGCGGGGTCTGAGTAGCCTTTGGGTGATAGATCTATACATAGTGATTTCATTGCGTGTATTT930.30777376973226994No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.29784558361187413No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.24820465300989508No Hit
TGTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.2151106992752424No Hit
TGTGCGGGGATAGCTGTACATCCGATACTGCTAATTACACACATGTGATGTTTTTGGGTTTGTT600.1985637224079161No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.1952543270344508No Hit
TGTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC460.15223218717940232No Hit
TGTGCGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.12906641956514545No Hit
TGTGCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.12906641956514545No Hit
TGTGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG370.12244762881821492No Hit
TGTGCGGGAACGTACACTTTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAGTGGCTTAC320.10590065195088857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAT150.00447683458.53523658
ACCCGAC201.9707716E-458.04825640
TGCCTAG201.9707716E-458.04825643
TTAAGCC201.9707716E-458.04825652
TAGAGGG201.9707716E-458.04825634
ATGGGCA150.00462776458.04825243
GTCACTA150.00462776458.04825231
GTTTGAT150.00462776458.04825248
CAGCATG150.00462776458.04825239
GGCACAT150.00462776458.04825246
CACATCA150.00462776458.04825248
ACTGACT150.00462776458.04825241
TGACTGC150.00462776458.04825243
CGGGGTG950.058.0482525
CGGGGTC501.8189894E-1258.0482525
TAAGCCA150.00462776458.04825253
GGGTACC303.6182428E-758.0482527
CAACTCC150.00462776458.04825253
TGCGAGG150.00462776458.0482523
ACTCCTA150.00462776458.04825255
Produced by FastQC (version 0.11.5)