FastQCFastQC Report
Fri 17 Jun 2016
SRR1526929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40118
Sequences flagged as poor quality0
Sequence length64
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG7271.8121541452714491No Hit
TGTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC6081.5155291888927664No Hit
TGTGCGGGGATAGCTGTACATCCGATACTGCTAATTACACACATGTGATGTTTTTGGGTTTGTT2640.6580587267560696No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2310.5758013859115608No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.5683234458347874No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1850.4611396380677003No Hit
TGTGCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1570.3913455306844808No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1520.3788822972231916No Hit
TGTGCGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC1500.3738970038386759No Hit
TGTGCGGGGTCTGAGTAGCCTTTGGGTGATAGATCTATACATAGTGATTTCATTGCGTGTATTT1380.34398524353158183No Hit
TGTGCGGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC1320.3290293633780348No Hit
TGTGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1260.31407348322448775No Hit
TGTGCGGGGATTGGCTTCATCCTTACCCTCAATGAAGGTGTGAAGGGGAAGAAGCTGACCTTCG1090.2716984894561045No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.2692058427638466No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.2592352559948153No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.22932349568772123No Hit
TGTGCGGGGGGGCGCCTCAGGAGCCTTCTTAAAGGGTTTGGAGAAGTGTTCGACCTGGTGGGCT880.2193529089186899No Hit
TGTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT810.20190438207288497No Hit
TGTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC800.19941173538062712No Hit
TGTGCGGGGTGCTCTCGGTCGAGGAAGCGCGCTCCGGAGCTAGCAGCGCCTCCTCACTCGAAGT710.1769779151503066No Hit
TGTGCGGGGAACACCTTATATCCCAAATGTGTCACTACCAGCATGGGCACATCAACTCCTACCT650.16202203499675957No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGATGGTCC640.15952938830450172No Hit
TGTGCGGGGGGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCCCG640.15952938830450172No Hit
TGTGCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC630.15703674161224387No Hit
TGTGCGGGGGACCTGCAGAGTCTTCAGGCTTGAGAGTGTGGGCCAGTGGGTAGGGGGCTGGGAC610.1520514482277282No Hit
TGTGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT580.14457350815095468No Hit
TGTGCGGGGGCCCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATC560.139588214766439No Hit
TGTGCGGGGACACAGCGCACAGGAGCCAGCTTGCGGCGCGCCCTGAAGAGGTTCTTGAGACACT560.139588214766439No Hit
TGTGCGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCTCAGAT550.13709556807418116No Hit
TGTGCGGGGACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGCGCCCAGC540.1346029213819233No Hit
TGTGCGGGGGAGTATGGCATCGAGGAGAATGGAGACCAAACCTGTGATAACCTGTCTCAAAACC540.1346029213819233No Hit
TGTGCGGGAACGTACACTTTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAGTGGCTTAC530.13211027468966546No Hit
TGTGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC520.12961762799740764No Hit
TGTGCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.12961762799740764No Hit
TGTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.1271249813051498No Hit
TGTGCGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG500.12463233461289197No Hit
TGTGCGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT470.11715439453611845No Hit
TGTGCGGGGAGGTAGCAATCGACCTCAGGAAATAGAAATTGGAGAATCTGGTTTTGCTTTATTA440.10967645445934494No Hit
TGTGCGGGGGGAGAGGAGGGGAGGCCGGAGGTGCCTGGGGCATCCTCTACTTTGGTGGCTCCAG440.10967645445934494No Hit
TGTGCGGGGCCTCCAAGACTTCATCAACATTAAGCTCAACTCAGCTTCTATCTTGCCAGAAATG420.10469116107482926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTTA150.004570320758.2666755
AGGCCCC406.6211214E-1058.2666758
CTGCCCG150.004570320758.2666758
CATGCAT351.5314072E-858.26666658
AATCTGG150.004593165658.1934745
AAGATTA351.5466867E-858.1934748
ATCTGGT150.004593165658.1934746
AAAGATT351.5466867E-858.1934747
GATGTTT258.3510095E-658.19346647
TGATGTT258.3510095E-658.19346646
CACACAT258.413577E-658.12045338
GCTAATA150.00461609558.12045341
AATAGAA150.00461609558.12045331
CTAATTA258.413577E-658.12045331
TAATTAC258.413577E-658.12045332
AATTGGA150.00461609558.12045337
GAAATAG150.0046391158.0476229
ACGTATG150.0046391158.0476229
CAACATG150.0046391158.0476228
GACGTAT150.0046391158.0476228
Produced by FastQC (version 0.11.5)