FastQCFastQC Report
Fri 17 Jun 2016
SRR1526928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40005
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG6651.6622922134733158No Hit
TGTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC5541.3848268966379202No Hit
TGTGCGGGGATAGCTGTACATCCGATACTGCTAATTACACACATGTGATGTTTTTGGGTTTGTT2720.679915010623672No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2360.5899262592175978No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.5674290713660792No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1590.3974503187101612No Hit
TGTGCGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC1480.3699537557805274No Hit
TGTGCGGGGTCTGAGTAGCCTTTGGGTGATAGATCTATACATAGTGATTTCATTGCGTGTATTT1370.34245719285089365No Hit
TGTGCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1360.339957505311836No Hit
TGTGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1280.3199600049993751No Hit
TGTGCGGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC1250.31246094238220223No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1230.30746156730408697No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.23497062867141608No Hit
TGTGCGGGGATTGGCTTCATCCTTACCCTCAATGAAGGTGTGAAGGGGAAGAAGCTGACCTTCG910.2274715660542432No Hit
TGTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT810.20247469066366702No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.19497562804649418No Hit
TGTGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT780.19497562804649418No Hit
TGTGCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC710.17747781527309087No Hit
TGTGCGGGGGGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCCCG690.17247844019497563No Hit
TGTGCGGGGTGCTCTCGGTCGAGGAAGCGCGCTCCGGAGCTAGCAGCGCCTCCTCACTCGAAGT640.15998000249968755No Hit
TGTGCGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCTCAGAT640.15998000249968755No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1549806274215723No Hit
TGTGCGGGGAACACCTTATATCCCAAATGTGTCACTACCAGCATGGGCACATCAACTCCTACCT610.15248093988251468No Hit
TGTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC590.14748156480439945No Hit
TGTGCGGGGGGGCGCCTCAGGAGCCTTCTTAAAGGGTTTGGAGAAGTGTTCGACCTGGTGGGCT580.14498187726534184No Hit
TGTGCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.14498187726534184No Hit
TGTGCGGGAACGTACACTTTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAGTGGCTTAC570.1424821897262842No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGATGGTCC560.13998250218722658No Hit
TGTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.137482814648169No Hit
TGTGCGGGGACACAGCGCACAGGAGCCAGCTTGCGGCGCGCCCTGAAGAGGTTCTTGAGACACT550.137482814648169No Hit
TGTGCGGGGGACCTGCAGAGTCTTCAGGCTTGAGAGTGTGGGCCAGTGGGTAGGGGGCTGGGAC540.13498312710911137No Hit
TGTGCGGGGACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGCGCCCAGC520.12998375203099613No Hit
TGTGCGGGGGAGTATGGCATCGAGGAGAATGGAGACCAAACCTGTGATAACCTGTCTCAAAACC520.12998375203099613No Hit
TGTGCGGGGGCCCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATC480.11998500187476566No Hit
TGTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC470.11748531433570804No Hit
TGTGCGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG440.10998625171853518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCA150.00450569358.47595239
CCACGAT150.00450569358.47595243
CACGATG150.00450569358.47595244
GCCACGA150.00450569358.47595242
CGGCCTA150.00450569358.47595245
ACGATGC150.00450569358.47595245
CACATGT550.058.4759540
TAGTGAT201.9079362E-458.4759542
TGATTTC201.9079362E-458.4759545
ACACATG550.058.4759539
ACATAGT201.9079362E-458.4759539
AGTGATT201.9079362E-458.4759543
GTGATTT201.9079362E-458.4759544
GTGATGT550.058.4759545
GGTGATA150.00457394558.25472625
CTGAGTT150.00457394558.25472626
AAATTAC150.00457394558.25472623
AATTACC150.00457394558.25472624
CGTGGGT150.00457394558.25472626
CCACCTG150.00457394558.25472624
Produced by FastQC (version 0.11.5)