SRR1526927_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526927_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35981
Sequences flagged as poor quality	0
Sequence length	56
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGTGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	526	1.4618826602929325	No Hit
TGTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	458	1.2728940274033518	No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	283	0.7865262221728133	No Hit
TGTGCGGGGATAGCTGTACATCCGATACTGCTAATTACACACATGTGATGTTTTTG	253	0.7031488841332926	No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	226	0.6281092798977238	No Hit
TGTGCGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCT	131	0.3640810427725744	No Hit
TGTGCGGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	130	0.36130179817125707	No Hit
TGTGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC	129	0.3585225535699397	No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	121	0.3362885967594008	No Hit
TGTGCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	118	0.32795086295544873	No Hit
TGTGCGGGGTCTGAGTAGCCTTTGGGTGATAGATCTATACATAGTGATTTCATTGC	109	0.3029376615435924	No Hit
TGTGCGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	104	0.28904143853700565	No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100	0.2779244601317362	No Hit
TGTGCGGGGATTGGCTTCATCCTTACCCTCAATGAAGGTGTGAAGGGGAAGAAGCT	88	0.24457352491592785	No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	78	0.21678107890275422	No Hit
TGTGCGGGGGGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTA	77	0.21400183430143688	No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73	0.2028848558961674	No Hit
TGTGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	71	0.1973263666935327	No Hit
TGTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	70	0.19454712209221534	No Hit
TGTGCGGGGTGCTCTCGGTCGAGGAAGCGCGCTCCGGAGCTAGCAGCGCCTCCTCA	64	0.17787165448431116	No Hit
TGTGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	64	0.17787165448431116	No Hit
TGTGCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	60	0.1667546760790417	No Hit
TGTGCGGGAACGTACACTTTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAG	58	0.161196186876407	No Hit
TGTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA	57	0.15841694227508962	No Hit
TGTGCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.1528584530724549	No Hit
TGTGCGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTC	53	0.14729996386982017	No Hit
TGTGCGGGGGGGCGCCTCAGGAGCCTTCTTAAAGGGTTTGGAGAAGTGTTCGACCT	52	0.14452071926850282	No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	51	0.14174147466718545	No Hit
TGTGCGGGGAACACCTTATATCCCAAATGTGTCACTACCAGCATGGGCACATCAAC	51	0.14174147466718545	No Hit
TGTGCGGGGGACCTGCAGAGTCTTCAGGCTTGAGAGTGTGGGCCAGTGGGTAGGGG	50	0.1389622300658681	No Hit
TGTGCGGGGCAGAGTGCAGTGCTTGTGCGGGGGCTCCTCCTCCTCCTCGCCCGGCC	49	0.13618298546455074	No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	49	0.13618298546455074	No Hit
TGTGCGGGGACACAGCGCACAGGAGCCAGCTTGCGGCGCGCCCTGAAGAGGTTCTT	49	0.13618298546455074	No Hit
TGTGCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	47	0.13062449626191602	No Hit
TGTGCGGGGGTGTGTGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG	45	0.12506600705928128	No Hit
TGTGCGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	44	0.12228676245796392	No Hit
TGTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	43	0.11950751785664657	No Hit
TGTGCGGGGGCCCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGC	42	0.11672827325532921	No Hit
TGTGCGGGGATTGCAGAGCTGTGAAACTGCTTGGGAGAAGAGAAGGTTGCTTTGAA	42	0.11672827325532921	No Hit
TGTGCGGGGGAGTATGGCATCGAGGAGAATGGAGACCAAACCTGTGATAACCTGTC	41	0.11394902865401184	No Hit
TGTGCGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.11116978405269447	No Hit
TGTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.11116978405269447	No Hit
TGTGCGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	38	0.10561129485005977	No Hit
TGTGCGGGGACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG	37	0.1028320502487424	No Hit
TGTGCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	37	0.1028320502487424	No Hit
TGTGCGGGGAGCCAGTCAGCGCTCATTTCCCTGCTGGAAGCTTGGCGGAGCCGGGG	36	0.10005280564742502	No Hit
TGTGCGGGGCCTCCAAGACTTCATCAACATTAAGCTCAACTCAGCTTCTATCTTGC	36	0.10005280564742502	No Hit
TGTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC	36	0.10005280564742502	No Hit
TGTGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	36	0.10005280564742502	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCAA	25	1.8896944E-5	50.677513	49
TTCATTG	15	0.007930659	50.67751	49
CCAGCGC	15	0.007930659	50.67751	49
TTTCCAC	15	0.007930659	50.67751	50
AGCGACT	15	0.007930659	50.67751	50
CGTCTCG	15	0.007930659	50.67751	50
GTCGGAG	15	0.007930659	50.67751	50
ATGTTTT	40	2.2992026E-9	50.605934	48
TGATGTT	35	4.900903E-8	50.251057	46
ATGCTTT	15	0.008200618	50.251053	46
GCGCGAT	15	0.008246268	50.18067	41
GAACCTG	15	0.008246268	50.18067	42
AGGCTCA	20	4.056392E-4	50.18067	42
GTGATGT	35	4.955473E-8	50.18067	45
CTCACAA	20	4.056392E-4	50.18067	45
CATGTGA	35	4.955473E-8	50.18067	42
TGTGATG	35	4.955473E-8	50.18067	44
TCCTCTT	15	0.008292106	50.11049	37
AAACTGG	15	0.008292106	50.11049	39
CACATGT	35	5.01077E-8	50.11049	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content