##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526927_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35981 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.574775575998444 39.0 38.0 40.0 36.0 40.0 2 38.12534393151941 39.0 38.0 40.0 35.0 40.0 3 37.963286178816595 39.0 38.0 40.0 35.0 40.0 4 38.29523915399794 39.0 38.0 40.0 35.0 40.0 5 38.133514910647285 39.0 38.0 40.0 35.0 40.0 6 38.27992551624468 39.0 38.0 40.0 35.0 40.0 7 38.5651871821239 39.0 38.0 40.0 36.0 40.0 8 38.546677413079124 39.0 38.0 40.0 36.0 40.0 9 37.90033628859676 39.0 38.0 40.0 35.0 40.0 10 37.20880464689697 39.0 35.0 40.0 32.0 40.0 11 37.49792946277202 39.0 37.0 40.0 33.0 40.0 12 37.684972624440675 39.0 38.0 40.0 33.0 40.0 13 37.55601567493955 39.0 38.0 40.0 33.0 40.0 14 37.77682665851422 39.0 38.0 40.0 34.0 40.0 15 37.81267891387121 39.0 38.0 40.0 34.0 40.0 16 37.635585447875265 39.0 38.0 40.0 33.0 40.0 17 37.57196853895111 39.0 38.0 40.0 33.0 40.0 18 37.46938662071649 39.0 37.0 40.0 33.0 40.0 19 37.386481754259194 39.0 37.0 40.0 33.0 40.0 20 37.329868541730356 39.0 37.0 40.0 33.0 40.0 21 37.27634029070899 39.0 37.0 40.0 33.0 40.0 22 37.10894638837164 39.0 36.0 40.0 33.0 40.0 23 36.9877713237542 39.0 36.0 40.0 33.0 40.0 24 36.801339595897836 39.0 36.0 40.0 32.0 40.0 25 36.75106306106 39.0 36.0 40.0 32.0 40.0 26 36.26085989827965 38.0 36.0 40.0 31.0 40.0 27 36.095411467163224 38.0 35.0 40.0 30.0 40.0 28 35.88382757566493 38.0 35.0 40.0 30.0 40.0 29 35.6453683888719 38.0 35.0 40.0 29.0 40.0 30 35.43464606320002 38.0 35.0 40.0 29.0 40.0 31 34.921486340012784 38.0 34.0 39.0 27.0 40.0 32 34.631722297879435 38.0 33.0 39.0 27.0 40.0 33 34.437147383341205 38.0 33.0 39.0 26.0 40.0 34 34.632917373058 38.0 34.0 40.0 26.0 40.0 35 34.287290514438176 38.0 33.0 39.0 25.0 40.0 36 33.869903560212336 38.0 33.0 40.0 22.0 40.0 37 33.58230732886801 38.0 33.0 39.0 20.0 40.0 38 33.238542564131066 38.0 33.0 39.0 18.0 40.0 39 33.1113921236208 38.0 33.0 39.0 17.0 40.0 40 33.00355743308969 37.0 33.0 39.0 14.0 40.0 41 32.40721491898502 37.0 32.0 39.0 4.0 40.0 42 32.354714988466135 37.0 32.0 39.0 4.0 40.0 43 32.12134181929352 36.0 31.0 39.0 4.0 40.0 44 31.77502014952336 36.0 31.0 39.0 4.0 40.0 45 31.457435868930826 36.0 31.0 39.0 4.0 40.0 46 30.917039548650678 36.0 30.0 39.0 4.0 40.0 47 30.62519107306634 36.0 29.0 39.0 4.0 40.0 48 30.35896723270615 35.0 29.0 39.0 4.0 40.0 49 30.034740557516468 35.0 29.0 39.0 4.0 40.0 50 29.554097996164643 35.0 28.0 39.0 4.0 40.0 51 28.755454267530084 35.0 26.0 39.0 4.0 40.0 52 28.60520830438287 35.0 26.0 39.0 4.0 40.0 53 28.074317000639226 34.0 23.0 39.0 4.0 40.0 54 27.501987159889943 34.0 17.0 38.0 4.0 40.0 55 27.174064089380508 34.0 4.0 38.0 4.0 40.0 56 16.153108585086574 20.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.2648261654250064 1 2 -0.2759519475373864 1 3 0.21330391044273966 1 4 -0.7316008251639161 1 5 -0.7462323486723861 1 6 -0.26740948164027145 1 7 -0.4088296753149834 1 8 -0.8070208787942192 1 9 -1.316663732679956 1 10 -1.3429507261546973 1 11 -0.9049123712045741 1 12 -0.09805484466458125 1 13 -0.3108191186061333 1 14 -1.0657882848287983 1 15 -0.6435489112148787 1 16 0.0993611635868561 1 17 -1.2037685539665404 1 18 -0.6236533896456109 1 19 -0.2747820786038062 1 20 -0.8207487547640184 1 21 -0.2461585521768228 1 22 -0.5410693055562774 1 23 -0.5472778217430587 1 24 -0.7931811613619928 1 25 -1.3408720460829215 1 26 -0.9935443689774459 1 27 -0.9060976176498556 1 28 -0.07999494310770672 1 29 -0.21968234922242402 1 30 0.9121944190230593 1 31 -0.9819350192795895 1 32 -1.0862010261539154 1 33 -0.2404499417224386 1 34 -0.875374482989578 1 35 -2.2249398524901736 1 36 0.7708352242959577 1 37 0.5491249521129618 1 38 0.018462079140029175 1 39 0.3065011064137053 1 40 0.5913727356860647 1 41 0.5738279941413964 1 42 0.8656908889393691 1 43 0.2294177218154232 1 44 0.6738742717446229 1 45 -1.3183020618086445 1 46 0.5207098292341996 1 47 1.5504610379016803 1 48 -1.1561966249925817 1 49 1.4425742434315225 1 50 -0.2128031660472125 1 51 0.968910193906531 1 52 -0.3152301431046922 1 53 0.46828190552259485 1 54 1.2017667344817795 1 55 2.1900267754572162 1 56 2.7550937142792833 2 1 -0.8052302058290408 2 2 -0.3668610384464799 2 3 -0.5796253824865545 2 4 -0.5194796130427051 2 5 -0.003808106248143872 2 6 -0.8305407947715793 2 7 -0.41893068541599376 2 8 -0.6731824949558387 2 9 -0.6196940357102534 2 10 -1.3126476958516662 2 11 -2.056427522719723 2 12 -1.0399740365837715 2 13 -0.9244554822425002 2 14 -0.5708387898793035 2 15 0.018067250401280432 2 16 -0.13548732126162832 2 17 -0.10275845295643649 2 18 -0.6867847027769258 2 19 0.5307734769517509 2 20 -0.21973865375392165 2 21 -0.29666360268187475 2 22 0.6356983712113973 2 23 0.05373227926703805 2 24 -0.42701954520038043 2 25 -0.4393568945677728 2 26 0.887768762335682 2 27 -0.5172087287609699 2 28 -0.5698939330066963 2 29 0.5277923982523234 2 30 -0.00699750016886469 2 31 0.3589740716294969 2 32 1.5829908930380086 2 33 0.274701573429077 2 34 1.8190699614548649 2 35 1.36596923841892 2 36 2.4223503758111065 2 37 1.2233673763553838 2 38 0.7911893518673025 2 39 2.7004405003530962 2 40 1.5560192003325284 2 41 1.1218077921211957 2 42 0.8884181616666424 2 43 1.5147712571689596 2 44 1.598116695987045 2 45 1.8811928876863035 2 46 2.702528011052383 2 47 2.010056997497646 2 48 2.1215811527851933 2 49 2.028432829290111 2 50 1.9639645107204657 2 51 1.9840617090580466 2 52 1.8918405639660136 2 53 3.331918269158958 2 54 3.6563121890272328 2 55 3.697602533032974 2 56 3.744992704178273 3 1 0.3462849456861079 3 2 0.696270274684835 3 3 0.29663724377607537 3 4 0.46284361928053386 3 5 0.5871009846609496 3 6 0.3714794072486214 3 7 0.39672588024057376 3 8 0.35964578787244506 3 9 0.15555848954226548 3 10 0.5181603849564169 3 11 -0.349356815649017 3 12 0.4297229331131973 3 13 0.6336253258383095 3 14 0.7675450485045374 3 15 0.41200664434067846 3 16 0.07158338580907753 3 17 -0.1759907761887618 3 18 0.09856883257661053 3 19 0.19744014361841522 3 20 0.6792512452359816 3 21 0.5593970033787272 3 22 0.8200418055548298 3 23 0.2582777338124913 3 24 0.5401521719713429 3 25 0.5757946205837428 3 26 1.700900075466997 3 27 0.9272357156834801 3 28 0.3366717235589647 3 29 0.4469843174442403 3 30 0.30108330791194504 3 31 0.018064980720410517 3 32 0.7471323071794203 3 33 -0.37933883061133145 3 34 -0.23648559410068515 3 35 -0.1138287413790593 3 36 0.9930574465181792 3 37 0.7713471743351832 3 38 1.157350968028922 3 39 0.8898344397470339 3 40 0.3691505134638362 3 41 1.7960502163636178 3 42 1.6712464444949262 3 43 0.9794177218154232 3 44 2.3127631606335086 3 45 1.7372534937469126 3 46 2.8818209403453103 3 47 2.7449054823461303 3 48 2.0382478194518647 3 49 3.025907576764858 3 50 3.537196833952791 3 51 1.941132416128756 3 52 2.5458809680064185 3 53 2.44050412774482 3 54 3.5351000678151117 3 55 2.356693442123884 3 56 1.6717603809459511 4 1 -0.5703817209805564 4 2 -0.24817416975960782 4 3 -0.3144738673350389 4 4 -1.5093786029416947 4 5 -1.2184545708946075 4 6 -0.6007428149736 4 7 -0.24216300864831908 4 8 -0.14035421212755494 4 9 -0.14999706601328455 4 10 -0.6485062817102545 4 11 -1.2938012600934599 4 12 -1.4591659557756884 4 13 -0.5608191186061333 4 14 -1.3713438403843554 4 15 -0.6157711334371001 4 16 -1.5673055030798082 4 17 -0.787101887299869 4 18 -1.484764500756718 4 19 -0.5525598563815848 4 20 -0.542970976986247 4 21 -0.7183807743990513 4 22 -1.6521804166673917 4 23 -0.7139444884097301 4 24 -1.904292272473107 4 25 -0.6186498238607001 4 26 -0.3268777023107816 4 27 1.0105690490168087 4 28 -0.30221716532992815 4 29 1.280317650777576 4 30 0.4399721968008379 4 31 -0.03749057483514662 4 32 1.4971323071794203 4 33 0.12066116938866855 4 34 1.0135144058993149 4 35 1.3306157030653836 4 36 -1.3402758868151565 4 37 -1.2564306034425954 4 38 -1.0648712541932994 4 39 -1.387943338030741 4 40 -1.241960597647271 4 41 -0.8428386725252714 4 42 -1.384309111060631 4 43 -1.0205822781845768 4 44 -1.6594590615887093 4 45 -0.3738576173641981 4 46 -0.5348457263213575 4 47 0.07823881567946245 4 48 -0.545085513881471 4 49 -0.835203534346256 4 50 -1.3794698327138804 4 51 0.8022435272398667 4 52 0.8792143013397542 4 53 0.6349485721892627 4 54 1.229544512259558 4 55 0.7455823310127734 4 56 -0.2726840634984953 5 1 -0.9965721971710337 5 2 -1.618809090394528 5 3 -0.7501881530493222 5 4 -0.8554103489734359 5 5 -1.0748037772438153 5 6 -0.60629837052916 5 7 -1.1167661832514924 5 8 -0.98400500577835 5 9 -1.4404732564894758 5 10 -0.9143792975832667 5 11 -1.2287218950140968 5 12 -1.2226580192677545 5 13 -1.0465334043204209 5 14 -0.9007089197494338 5 15 -1.4356124032783697 5 16 -1.1077816935559994 5 17 -0.27678442698240957 5 18 -1.2815898975821156 5 19 -0.8692265230482477 5 20 -1.0318598658751341 5 21 0.15701605099777538 5 22 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1.7792388104428518 6 49 1.5837153845726633 6 50 1.2841938309497856 6 51 3.7842255092218444 6 52 4.452037124162576 6 53 3.8511647884054803 6 54 4.309874842589885 6 55 2.9039907394211824 6 56 2.1004390596246303 7 1 0.12047849407320399 7 2 0.014370633107773756 7 3 0.5806874229875447 7 4 0.2468937984920032 7 5 0.3344128126179342 7 6 0.27112098430955456 7 7 0.46572229601117954 7 8 0.3551655011340955 7 9 0.6940889554920915 7 10 1.2054363706194948 7 11 0.8800338653545694 7 12 0.06950787934975722 7 13 0.8262776555874183 7 14 0.12417587287729503 7 15 -0.11487507608943304 7 16 -0.10225173963895173 7 17 0.35178700158901677 7 18 0.5976727752289435 7 19 -0.7272910391772811 7 20 0.7939465857377712 7 21 0.5459561431636786 7 22 0.5252389381713201 7 23 0.7770949381135708 7 24 0.14678299634410052 7 25 0.6241817173579349 7 26 1.4840541973307921 7 27 1.2032213787659174 7 28 1.5239477092220426 7 29 1.4828266113510509 7 30 -0.19174823330669 7 31 2.515376808677395 7 32 1.7032254971435776 7 33 2.006861886234546 7 34 2.7007903915623928 7 35 2.06000638406897 7 36 4.045028772683054 7 37 4.594823876844146 7 38 2.9485696060217492 7 39 2.9731677730803696 7 40 3.077035818123335 7 41 3.9976631195894257 7 42 4.744723147003889 7 43 3.8862277576577213 7 44 3.464196852389783 7 45 4.842092203424329 7 46 4.918559291599788 7 47 5.580926987722474 7 48 5.616204808699177 7 49 5.406731949524712 7 50 4.498666368002965 7 51 4.7807381508957825 7 52 5.976884552235813 7 53 5.3123679270279744 7 54 4.104992540933392 7 55 4.477661184059368 7 56 3.016742459798998 8 1 -2.341215054313892 8 2 -2.7447019475373864 8 3 -3.251973867335039 8 4 -2.5545174918305804 8 5 -3.683732348672386 8 6 -1.5312983705291572 8 7 -1.6623018975372048 8 8 -1.1438264343497764 8 9 -2.7645803993466203 8 10 -2.9367007261546973 8 11 -1.7590790378712384 8 12 -1.768193733553467 8 13 -1.2483191186061333 8 14 -1.9060660626065768 8 15 -1.6713266889926572 8 16 -1.338138836413144 8 17 -1.8044629984109832 8 18 -2.4396256118678323 8 19 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3.3729677794611987 120 46 4.025471733996103 120 47 2.0472864347270807 120 48 2.540628771832818 120 49 3.3790821799394593 120 50 3.4332285799845366 120 51 3.849862574858914 120 52 2.9879444600699117 120 53 3.6349485721892627 120 54 2.344623877338922 120 55 1.8924077278381688 120 56 -0.009985650800082624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 19.0 5 2.0 6 6.0 7 6.0 8 20.0 9 31.0 10 19.0 11 33.0 12 36.0 13 65.0 14 74.0 15 115.0 16 114.0 17 170.0 18 190.0 19 265.0 20 255.0 21 318.0 22 337.0 23 418.0 24 569.0 25 714.0 26 511.0 27 573.0 28 680.0 29 733.0 30 883.0 31 1104.0 32 1302.0 33 1584.0 34 2097.0 35 2697.0 36 3629.0 37 5228.0 38 6234.0 39 4950.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.39968316611545 0.45857535921736475 0.09449431644479031 0.047247158222395155 8 98.98532191704659 0.7505837873901924 0.13065717780495942 0.13343711775825642 9 94.41093910675077 3.062727550651733 1.6453128039798783 0.8810205386176038 10 48.99252383202246 31.808454462077208 8.307162113337595 10.891859592562742 11 32.61443539645924 27.622912092493262 19.551985770267642 20.210666740779857 12 35.24638003390678 17.053444873683333 23.990439398571468 23.709735693838415 13 25.074344793085242 17.40362969344932 28.79019482504655 28.731830688418885 14 22.2506322781468 19.94385925905339 30.39659820460799 27.40891025819182 15 29.82407381673661 19.129540590867403 27.0364914816153 24.00989411078069 16 29.26544565187182 19.832689475000695 24.788082599149554 26.113782273977932 17 28.74572691142547 20.930491092521052 23.22058864400656 27.10319335204691 18 28.881909896890022 22.86206609043662 22.72032461576943 25.535699396903922 19 28.259359106194935 20.583085517356384 24.401767599566437 26.755787776882244 20 26.299991662266198 21.800394652733388 24.56852227564548 27.331091409354936 21 29.245990939662597 18.387482282315666 26.277757705455656 26.088769072566077 22 30.238181262332898 18.912759511964648 25.02153914566021 25.827520080042245 23 30.50776798866068 21.013868430560574 24.465690225396738 24.01267335538201 24 25.383546414674818 25.708727070594772 23.068371317398555 25.839355197331855 25 28.5487950635128 20.821636045251132 22.01962364844206 28.609945242794005 26 25.83829171995774 19.86598454095535 26.241450258577547 28.054273480509366 27 27.10412901431948 19.430001390240513 26.795495620742386 26.670373974697625 28 28.769294952023362 19.60506188290919 24.041162564316508 27.584480600750936 29 26.863678103347528 22.046915435345188 26.774633386203632 24.314773075103655 30 27.653530149004318 19.66021445481131 29.363598384626094 23.322657011558277 31 26.060910028142324 19.89188887960099 28.008582016773943 26.03861907548274 32 28.246635646819534 19.857345852720737 29.957370928645066 21.938647571814663 33 29.155867681074604 20.469303012568627 29.61848228966363 20.75634701669314 34 23.80700189541755 21.85026201360241 33.60185081948936 20.74088527149069 35 22.50383382127422 23.93977415307403 34.5629443747386 18.993447650913147 36 25.287324257978128 21.072305288997992 30.938964516848916 22.70140593617496 37 24.14495341181722 23.302460525581655 31.52931986832562 21.02326619427551 38 28.54790544500572 20.306438558789875 32.71747927772041 18.428176718483993 39 24.863159070598748 20.702077747989275 31.182975871313673 23.2517873100983 40 25.147408131898842 20.70420567276792 31.309207768618137 22.839178426715105 41 21.912907373246128 22.866007043434514 32.22091788249762 23.000167700821734 42 21.541385961479328 19.51471780393034 31.957062589103515 26.98683364548682 43 22.869127516778523 20.04194630872483 34.96364653243848 22.125279642058164 44 24.735031740261192 19.992169803406135 32.66869878911602 22.604099667216644 45 24.160116366891383 20.4816917955747 31.847044672578257 23.511147164955663 46 25.895555804321056 20.007276390910107 31.618717116310307 22.478450688458523 47 23.602710573476703 21.172155017921146 34.16778673835125 21.057347670250895 48 21.63949123101922 22.984255056872303 31.89051381184513 23.48573990026335 49 22.80249782980034 23.844193665817258 31.922936910195737 21.430371594186663 50 21.53846153846154 25.39442342721031 31.55667647470926 21.510438559618887 51 22.46813279170752 25.871970864266704 32.26502311248074 19.394873231545034 52 24.626510076519885 22.563556352832357 31.33951845726939 21.470415113378365 53 24.051023268853378 22.354920100925145 32.67731987664704 20.916736753574433 54 24.77548715052245 23.42841005365716 31.781982490821804 20.01412030499859 55 25.442459260090317 22.758253162426726 29.82918688469414 21.970100692788826 56 24.274458121862995 22.367720045986037 30.028320668479935 23.329501163671033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.5 9 2.0 10 15.0 11 28.0 12 59.5 13 58.5 14 26.0 15 27.0 16 28.0 17 45.5 18 63.0 19 130.5 20 198.0 21 254.0 22 331.5 23 353.0 24 416.0 25 479.0 26 559.0 27 639.0 28 778.0 29 951.5 30 986.0 31 1001.5 32 1017.0 33 965.5 34 914.0 35 836.5 36 759.0 37 755.5 38 578.5 39 405.0 40 412.5 41 420.0 42 581.5 43 743.0 44 733.5 45 724.0 46 668.0 47 769.5 48 927.0 49 856.5 50 786.0 51 878.0 52 970.0 53 1075.5 54 1394.0 55 1607.0 56 1595.5 57 1584.0 58 1705.0 59 1826.0 60 1876.5 61 1927.0 62 2237.5 63 2569.0 64 2590.0 65 2360.5 66 2131.0 67 2024.0 68 1917.0 69 1733.5 70 1550.0 71 1734.5 72 1346.0 73 773.0 74 653.0 75 533.0 76 432.0 77 331.0 78 261.5 79 147.0 80 102.0 81 81.0 82 60.0 83 48.0 84 36.0 85 24.0 86 12.0 87 10.0 88 6.0 89 4.0 90 2.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.025013201411856256 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.002779244601317362 25 0.011116978405269448 26 0.04168866901976043 27 0.04446791362107779 28 0.07226035963425141 29 0.12228676245796392 30 0.21122258970011953 31 0.2556905033211973 32 0.25013201411856256 33 0.2723659709291015 34 0.291820683138323 35 0.322392373752814 36 0.36963953197520916 37 0.3751980211778439 38 0.41688669019760427 39 0.4808093160279036 40 0.544731941858203 41 0.5641866540674245 42 0.5780828770740113 43 0.614213056891137 44 0.6169923014924543 45 0.644784747505628 46 0.6920319057280231 47 0.7476167977543704 48 0.7976432005780829 49 0.7503960423556878 50 0.8226564019899392 51 0.7948639559767654 52 0.8448903588004781 53 0.8643450710096996 54 1.5869486673522135 55 0.9115922292320947 56 0.883799783218921 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 35981.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.654651065840305 #Duplication Level Percentage of deduplicated Percentage of total 1 70.99287757959458 32.41155054056308 2 12.38205393559384 11.305967038159029 3 5.825774639313326 7.9792112503821455 4 3.0985572533024897 5.658542008282149 5 1.9723625738114081 4.502376254134126 6 1.412309003469897 3.868708485033768 7 0.9618311316734645 3.0738445290570025 8 0.6087538808059901 2.2233956810538897 9 0.4565654106044926 1.8759901058892192 >10 2.112375966396786 16.789416636558183 >50 0.10348815973701832 3.11831244267808 >100 0.06696292688865892 5.7308023679164 >500 0.006087538808059901 1.4618826602929325 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 526 1.4618826602929325 No Hit TGTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG 458 1.2728940274033518 No Hit TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 283 0.7865262221728133 No Hit TGTGCGGGGATAGCTGTACATCCGATACTGCTAATTACACACATGTGATGTTTTTG 253 0.7031488841332926 No Hit TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 226 0.6281092798977238 No Hit TGTGCGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCT 131 0.3640810427725744 No Hit TGTGCGGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 130 0.36130179817125707 No Hit TGTGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC 129 0.3585225535699397 No Hit TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 121 0.3362885967594008 No Hit TGTGCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 118 0.32795086295544873 No Hit TGTGCGGGGTCTGAGTAGCCTTTGGGTGATAGATCTATACATAGTGATTTCATTGC 109 0.3029376615435924 No Hit TGTGCGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 104 0.28904143853700565 No Hit TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.2779244601317362 No Hit TGTGCGGGGATTGGCTTCATCCTTACCCTCAATGAAGGTGTGAAGGGGAAGAAGCT 88 0.24457352491592785 No Hit TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 78 0.21678107890275422 No Hit TGTGCGGGGGGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTA 77 0.21400183430143688 No Hit TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.2028848558961674 No Hit TGTGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 71 0.1973263666935327 No Hit TGTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 70 0.19454712209221534 No Hit TGTGCGGGGTGCTCTCGGTCGAGGAAGCGCGCTCCGGAGCTAGCAGCGCCTCCTCA 64 0.17787165448431116 No Hit TGTGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA 64 0.17787165448431116 No Hit TGTGCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 60 0.1667546760790417 No Hit TGTGCGGGAACGTACACTTTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAG 58 0.161196186876407 No Hit TGTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 57 0.15841694227508962 No Hit TGTGCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55 0.1528584530724549 No Hit TGTGCGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTC 53 0.14729996386982017 No Hit TGTGCGGGGGGGCGCCTCAGGAGCCTTCTTAAAGGGTTTGGAGAAGTGTTCGACCT 52 0.14452071926850282 No Hit TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 51 0.14174147466718545 No Hit TGTGCGGGGAACACCTTATATCCCAAATGTGTCACTACCAGCATGGGCACATCAAC 51 0.14174147466718545 No Hit TGTGCGGGGGACCTGCAGAGTCTTCAGGCTTGAGAGTGTGGGCCAGTGGGTAGGGG 50 0.1389622300658681 No Hit TGTGCGGGGCAGAGTGCAGTGCTTGTGCGGGGGCTCCTCCTCCTCCTCGCCCGGCC 49 0.13618298546455074 No Hit TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 49 0.13618298546455074 No Hit TGTGCGGGGACACAGCGCACAGGAGCCAGCTTGCGGCGCGCCCTGAAGAGGTTCTT 49 0.13618298546455074 No Hit TGTGCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 47 0.13062449626191602 No Hit TGTGCGGGGGTGTGTGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG 45 0.12506600705928128 No Hit TGTGCGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 44 0.12228676245796392 No Hit TGTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 43 0.11950751785664657 No Hit TGTGCGGGGGCCCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGC 42 0.11672827325532921 No Hit TGTGCGGGGATTGCAGAGCTGTGAAACTGCTTGGGAGAAGAGAAGGTTGCTTTGAA 42 0.11672827325532921 No Hit TGTGCGGGGGAGTATGGCATCGAGGAGAATGGAGACCAAACCTGTGATAACCTGTC 41 0.11394902865401184 No Hit TGTGCGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.11116978405269447 No Hit TGTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.11116978405269447 No Hit TGTGCGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 38 0.10561129485005977 No Hit TGTGCGGGGACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG 37 0.1028320502487424 No Hit TGTGCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 37 0.1028320502487424 No Hit TGTGCGGGGAGCCAGTCAGCGCTCATTTCCCTGCTGGAAGCTTGGCGGAGCCGGGG 36 0.10005280564742502 No Hit TGTGCGGGGCCTCCAAGACTTCATCAACATTAAGCTCAACTCAGCTTCTATCTTGC 36 0.10005280564742502 No Hit TGTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC 36 0.10005280564742502 No Hit TGTGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 36 0.10005280564742502 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.005558489202634724 0.0 0.0 0.0 0.0 12 0.011116978405269448 0.0 0.0 0.0 0.0 13 0.011116978405269448 0.0 0.0 0.0 0.0 14 0.01667546760790417 0.0 0.0 0.0 0.0 15 0.025013201411856256 0.0 0.0 0.0 0.0 16 0.03057169061449098 0.0 0.0 0.0 0.0 17 0.03335093521580834 0.0 0.0 0.0 0.0 18 0.03335093521580834 0.0 0.0 0.0 0.0 19 0.03335093521580834 0.0 0.0 0.0 0.0 20 0.036130179817125706 0.0 0.0 0.0 0.0 21 0.03890942441844307 0.0 0.0 0.0 0.0 22 0.05280564742502988 0.0 0.0 0.0 0.0 23 0.0583641366276646 0.0 0.0 0.0 0.0 24 0.07503960423556877 0.0 0.0 0.0 0.0 25 0.0805980934382035 0.0 0.0 0.0 0.0 26 0.0805980934382035 0.0 0.0 0.0 0.0 27 0.08893582724215558 0.0 0.0 0.0 0.0 28 0.1028320502487424 0.0 0.0 0.0 0.0 29 0.11116978405269448 0.0 0.0 0.0 0.0 30 0.11394902865401184 0.0 0.0 0.0 0.0 31 0.11394902865401184 0.0 0.0 0.0 0.0 32 0.11950751785664657 0.0 0.0 0.0 0.0 33 0.12506600705928128 0.0 0.0 0.0 0.0 34 0.12506600705928128 0.0 0.0 0.0 0.0 35 0.1389622300658681 0.0 0.0 0.0 0.0 36 0.14452071926850282 0.0 0.0 0.0 0.0 37 0.14452071926850282 0.0 0.0 0.0 0.0 38 0.1472999638698202 0.0 0.0 0.0 0.0 39 0.1472999638698202 0.0 0.0 0.0 0.0 40 0.15563769767377228 0.0 0.0 0.0 0.0 41 0.161196186876407 0.0 0.0 0.0 0.0 42 0.1667546760790417 0.0 0.0 0.0 0.0 43 0.1667546760790417 0.0 0.0 0.0 0.0 44 0.16953392068035908 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACCAA 25 1.8896944E-5 50.677513 49 TTCATTG 15 0.007930659 50.67751 49 CCAGCGC 15 0.007930659 50.67751 49 TTTCCAC 15 0.007930659 50.67751 50 AGCGACT 15 0.007930659 50.67751 50 CGTCTCG 15 0.007930659 50.67751 50 GTCGGAG 15 0.007930659 50.67751 50 ATGTTTT 40 2.2992026E-9 50.605934 48 TGATGTT 35 4.900903E-8 50.251057 46 ATGCTTT 15 0.008200618 50.251053 46 GCGCGAT 15 0.008246268 50.18067 41 GAACCTG 15 0.008246268 50.18067 42 AGGCTCA 20 4.056392E-4 50.18067 42 GTGATGT 35 4.955473E-8 50.18067 45 CTCACAA 20 4.056392E-4 50.18067 45 CATGTGA 35 4.955473E-8 50.18067 42 TGTGATG 35 4.955473E-8 50.18067 44 TCCTCTT 15 0.008292106 50.11049 37 AAACTGG 15 0.008292106 50.11049 39 CACATGT 35 5.01077E-8 50.11049 40 >>END_MODULE