FastQCFastQC Report
Fri 17 Jun 2016
SRR1526926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50941
Sequences flagged as poor quality0
Sequence length64
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13302.6108635480261477No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10161.9944641840560648No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8571.6823383914724876No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5891.1562395712687226No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.9540448754441412No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.500579101313284No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.43579827643744723No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.4318721658389117No Hit
GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1890.3710174515616105No Hit
GCTGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.321941069079916No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.3199780137806482No Hit
GCTGTGGGGGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.29642135018943483No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.28856912899236375No Hit
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.24341885710920477No Hit
GCTGTGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.2316405253135981No Hit
GCTGTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.22575135941579472No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.2198621935179914No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.20808386172238474No Hit
GCTGTGGGGGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.19434247462751025No Hit
GCTGTGGGGGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.18649025343043915No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.17667497693410023No Hit
GCTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.1707858110362969No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.15311831334288686No Hit
GCTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.1511552580436191No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.1393769262480124No Hit
GCTGTGGGGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1275985944524057No Hit
GCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1275985944524057No Hit
GCTGTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.10600498616046014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGGA201.9833868E-458.051185
GGGAGAC150.004645286658.051187
GGGACTC150.004645286658.051187
GGGATGT150.004645286658.051187
TGAGGGC150.004645286658.051185
GGAGGAG201.9833868E-458.051186
GGGGACG201.9833868E-458.051186
GGCCTAT150.0046634457.99418
GGCGGGT201.9930737E-457.99418
GACAGTG351.6136255E-857.937139
AGTAGTA150.00468164657.9371314
GCCTATC150.00468164657.937139
CAGTAGT150.00468164657.9371313
TAGTATC150.00468164657.9371316
AGAGAGC150.00468164657.9371328
CCGGGTA150.00468164657.9371329
GTAGTAT150.00468164657.9371315
GAGATCG150.00468164657.9371318
AGCAGTA258.606807E-657.93712611
GAACATG258.606807E-657.9371269
Produced by FastQC (version 0.11.5)