FastQCFastQC Report
Fri 17 Jun 2016
SRR1526925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62617
Sequences flagged as poor quality0
Sequence length64
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20903.337751728763754No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15542.481754156219557No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13442.1463819729466436No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8381.338294712298577No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7911.263235223661306No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.5509685868055001No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.49187920213360586No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.42799878627209864No Hit
GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.40404363032403345No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.38487950556558126No Hit
GCTGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2370.3784914639794305No Hit
GCTGTGGGGGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.3305811520833001No Hit
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.32579012089368703No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.3130140377213856No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.28905888177332034No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.27149176741140585No Hit
GCTGTGGGGGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.26829774661833045No Hit
GCTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.22837248670488844No Hit
GCTGTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.22198444511873772No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.22038743472220004No Hit
GCTGTGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.19483526837759713No Hit
GCTGTGGGGGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA1120.17886516441222033No Hit
GCTGTGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.1644920708433812No Hit
GCTGTGGGGGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.15331299806761742No Hit
GCTGTGGGGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.15011897727454207No Hit
GCTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG920.1469249564814667No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.14373093568839135No Hit
GCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.14373093568839135No Hit
GCTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.13734289410224063No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.1277608317230145No Hit
GCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTG790.12616382132647683No Hit
GCTGTGGGGGCTGCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.12456681092993915No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.11338773815417538No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC690.11019371736110002Illumina Single End Adapter 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCCG201.9566031E-458.23169758
CCATGCA150.004593364458.23169757
TCTCCCC201.9566031E-458.23169757
AGACACG150.004593364458.23169755
CATGCAT150.004593364458.23169758
TTGTCTC150.00460802958.18488740
AAACTGG150.00460802958.18488739
GTTTGAT150.00460802958.18488748
CGGAGAC150.00460802958.18488752
TGTCTCA150.00460802958.18488741
GTACTGA150.00460802958.18488743
ACGTACT150.00460802958.18488741
TCGGAGA150.00460802958.18488751
TACGTAG150.00460802958.18488740
TACGTAC150.00460802958.18488740
ATTAAGC150.00460802958.18488751
CGTACTG150.00460802958.18488742
GTCTCAA150.00460802958.18488742
TACTGAG150.00460802958.18488744
CAAAGAT150.00460802958.18488746
Produced by FastQC (version 0.11.5)