FastQCFastQC Report
Fri 17 Jun 2016
SRR1526924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62012
Sequences flagged as poor quality0
Sequence length64
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19693.1751918983422565No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14662.364058569309166No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11401.8383538669934851No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7821.2610462491130747No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7621.228794426885119No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2880.46442624008256467No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.4515255111913823No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.4370121911888022No Hit
GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.3934722311810617No Hit
GCTGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.3773463200670838No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.34509449783912793No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.33703154228213894No Hit
GCTGTGGGGGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.3096174933883764No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.27575308004902277No Hit
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.274140488937625No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.25478939560085145No Hit
GCTGTGGGGGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.2531768044894537No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.19351093336773528No Hit
GCTGTGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.1870605689221441No Hit
GCTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.1854479778107463No Hit
GCTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1080.17415984003096174No Hit
GCTGTGGGGGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA1020.164484293362575No Hit
GCTGTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.1548087466941882No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.14835838224859704No Hit
GCTGTGGGGGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.14352060891440366No Hit
GCTGTGGGGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.14190801780300585No Hit
GCTGTGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.1370702444688125No Hit
GCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.13384506224601692No Hit
GCTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.1290072889118235No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.12578210668902792No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC770.12416951557763013Illumina Single End Adapter 2 (96% over 31bp)
GCTGTGGGGGCTGCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.11288137779784557No Hit
GCTGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT650.1048184222408566No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.10159324001806103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATTCT150.00452418658.4542541
CGTGCGA150.00452418658.4542542
ACGTACT258.185001E-658.45424741
TACGTAC258.185001E-658.45424740
CGTACTG258.185001E-658.45424742
CTACGTA258.185001E-658.45424739
CTACAGC150.00465748858.02757657
TCTCCCC201.9907343E-458.02757657
TCTACGT258.590228E-657.98055338
CTCTACG258.590228E-657.98055337
AGGGAGG150.00467247857.98055351
GGATCTT150.00474796757.746578
GTGGGTT150.00474796757.746574
GGGACGC150.00474796757.746577
GCCACAC150.00474796757.7465719
TGGGTCA150.00474796757.746575
TGATGGC150.00476317357.70000514
GTATCCA150.00476317357.70000512
GCGTGAA150.00476317357.70000510
CCATCTC303.8387043E-757.70000510
Produced by FastQC (version 0.11.5)