FastQCFastQC Report
Fri 17 Jun 2016
SRR1526923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52160
Sequences flagged as poor quality0
Sequence length56
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16073.08090490797546No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA14382.7569018404907975No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11012.1108128834355826No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9381.7983128834355828No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA7311.401457055214724No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA2840.5444785276073619No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.5291411042944785No Hit
GCTGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.5272239263803681No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.5157208588957055No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1880.36042944785276076No Hit
GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.3585122699386503No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA1850.3546779141104294No Hit
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.3125No Hit
GCTGTGGGGGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.30866564417177916No Hit
GCTGTGGGGGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.2933282208588957No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.2741564417177914No Hit
GCTGTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.2703220858895706No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.2684049079754601No Hit
GCTGTGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.2147239263803681No Hit
GCTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.20897239263803682No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA1000.19171779141104295No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1000.19171779141104295No Hit
GCTGTGGGGGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAA820.15720858895705522No Hit
GCTGTGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAA800.15337423312883436No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA760.14570552147239263No Hit
GCTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC760.14570552147239263No Hit
GCTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.1418711656441718No Hit
GCTGTGGGGGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.1418711656441718No Hit
GCTGTGGGGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.13995398773006135No Hit
GCTGTGGGGGCTGCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA650.12461656441717792No Hit
GCTGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG630.12078220858895705No Hit
GCTGTGGGGAGTTCTATGGAGGTAAAATGTATTGGCTATAGAAGACAATAAGGGAG620.11886503067484663No Hit
GCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.11886503067484663No Hit
GCTGTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG590.11311349693251534No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA590.11311349693251534No Hit
GCTGTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG590.11311349693251534No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA570.10927914110429447No Hit
GCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACCCTGGAGCACTC550.10544478527607362No Hit
GCTGTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10161042944785276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGAT150.0083076750.11872536
TCCGACT150.00833945450.07039630
CTCCGAC150.00833945450.07039629
GCCGGGT150.0083713350.0221628
GGGCATC150.00853206549.782367
ATCTCAT402.7011993E-949.7823612
GGACTGG150.00853206549.782368
GAAGCTA150.00853206549.782369
GAAGTGG402.7011993E-949.782369
CATCTCA402.7011993E-949.7823611
GGATCTA150.00853206549.782368
GGACATC150.00853206549.782368
CCATCTC402.7011993E-949.7823610
GGAAGTA150.00853206549.782368
AAGCGTG150.00853206549.7823610
GGAAGGT150.00853206549.782368
GGGACTA204.2371897E-449.782367
GGGACGC355.340371E-849.782367
GGGATGT150.00853206549.782367
GCCATCT402.7011993E-949.782369
Produced by FastQC (version 0.11.5)