##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526923_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52160 Sequences flagged as poor quality 0 Sequence length 56 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.15561733128834 39.0 38.0 40.0 35.0 40.0 2 37.55308665644172 39.0 38.0 40.0 33.0 40.0 3 37.81434049079755 39.0 38.0 40.0 35.0 40.0 4 37.79279141104294 39.0 38.0 40.0 35.0 40.0 5 37.16903757668712 39.0 37.0 40.0 33.0 40.0 6 37.87689800613497 39.0 38.0 40.0 35.0 40.0 7 38.0767254601227 39.0 38.0 40.0 35.0 40.0 8 38.0052722392638 39.0 38.0 40.0 35.0 40.0 9 37.13289877300613 39.0 35.0 40.0 31.0 40.0 10 36.21104294478528 39.0 35.0 40.0 30.0 40.0 11 36.369363496932515 39.0 35.0 40.0 31.0 40.0 12 36.5034509202454 39.0 35.0 40.0 31.0 40.0 13 36.227204754601225 39.0 35.0 39.0 30.0 40.0 14 36.66527990797546 38.0 36.0 40.0 31.0 40.0 15 36.77611196319018 39.0 36.0 40.0 32.0 40.0 16 36.408742331288344 38.0 36.0 40.0 31.0 40.0 17 36.420437116564415 38.0 36.0 40.0 31.0 40.0 18 36.2865222392638 38.0 36.0 39.0 31.0 40.0 19 36.30162960122699 38.0 35.0 40.0 31.0 40.0 20 36.37852760736196 38.0 36.0 40.0 31.0 40.0 21 36.14503450920245 38.0 35.0 40.0 30.0 40.0 22 35.77335122699387 38.0 35.0 40.0 29.0 40.0 23 35.805176380368096 38.0 35.0 39.0 29.0 40.0 24 35.786848159509205 38.0 35.0 39.0 29.0 40.0 25 35.58071319018405 38.0 35.0 39.0 29.0 40.0 26 34.94476610429448 38.0 35.0 39.0 27.0 40.0 27 35.23125 38.0 35.0 39.0 28.0 40.0 28 34.932726226993864 38.0 34.0 39.0 27.0 40.0 29 34.81418711656442 38.0 34.0 39.0 27.0 40.0 30 34.25446702453988 38.0 33.0 39.0 25.0 40.0 31 33.858454754601226 38.0 33.0 39.0 22.0 40.0 32 33.7410467791411 38.0 33.0 39.0 22.0 40.0 33 33.897315950920245 38.0 33.0 39.0 23.0 40.0 34 33.83661809815951 38.0 33.0 39.0 22.0 40.0 35 33.50088190184049 38.0 33.0 39.0 20.0 40.0 36 33.55092024539877 38.0 34.0 39.0 13.0 40.0 37 33.41821319018405 38.0 34.0 39.0 13.0 40.0 38 33.21575920245399 38.0 33.0 39.0 9.0 40.0 39 33.071549079754604 38.0 33.0 39.0 4.0 40.0 40 32.84760352760736 38.0 33.0 39.0 4.0 40.0 41 32.63935966257669 38.0 33.0 39.0 4.0 40.0 42 32.470264570552146 38.0 33.0 39.0 4.0 40.0 43 32.3253259202454 38.0 32.0 39.0 4.0 40.0 44 32.06531825153374 38.0 32.0 39.0 4.0 40.0 45 31.829064417177914 37.0 32.0 39.0 4.0 40.0 46 31.29532208588957 37.0 32.0 39.0 4.0 40.0 47 31.10795628834356 37.0 31.0 39.0 4.0 40.0 48 30.829812116564415 37.0 31.0 39.0 4.0 40.0 49 30.55293328220859 36.0 30.0 39.0 4.0 40.0 50 30.238324386503066 36.0 29.0 39.0 4.0 40.0 51 29.50101610429448 36.0 28.0 39.0 4.0 40.0 52 29.247105061349693 35.0 27.0 39.0 4.0 40.0 53 28.890164877300613 35.0 24.0 39.0 4.0 40.0 54 28.310889570552145 35.0 15.0 39.0 4.0 40.0 55 27.8611004601227 35.0 4.0 39.0 4.0 40.0 56 16.319574386503067 20.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.16539967046757198 1 2 0.18239433625436874 1 3 0.12790385314351482 1 4 0.15610850963138034 1 5 -1.0010925422022368 1 6 0.19086725284006434 1 7 0.05412387781613148 1 8 -0.1670700139746515 1 9 -0.8337767830105776 1 10 -0.4844711201531098 1 11 0.09772875788095092 1 12 0.2458456919428258 1 13 0.5192060282949313 1 14 -0.26655321433089796 1 15 -1.3423239443329251 1 16 0.3751525201275214 1 17 -0.5217033485408464 1 18 0.34251490550644803 1 19 -0.6380549547818646 1 20 0.22592022619720353 1 21 -0.12405237704835059 1 22 -0.030272268014535086 1 23 -0.43172384392245533 1 24 -0.5193145844746923 1 25 -0.7461448772843084 1 26 0.8642237082143112 1 27 -0.39027463555994046 1 28 0.33392215447467066 1 29 0.4059066428903009 1 30 0.49926537393383796 1 31 1.004159768804648 1 32 0.8308887408019174 1 33 1.5443731638848561 1 34 -0.31561848884594923 1 35 1.2752626312843347 1 36 1.4161967596897327 1 37 0.9855761056305923 1 38 1.1375675355269905 1 39 1.1926455637320856 1 40 0.8213675660274475 1 41 0.7557815777166184 1 42 -0.23845263435502773 1 43 0.03564988004917069 1 44 0.8086444073582655 1 45 -0.19575752140223202 1 46 -1.3495616079399682 1 47 -1.1537356842353113 1 48 -0.9168527844709047 1 49 -0.5815138165157272 1 50 -0.8876322559393444 1 51 -1.223363683721665 1 52 -1.4568743777588686 1 53 -1.3483707047705167 1 54 -1.3615177359857071 1 55 -0.9884911455893253 1 56 0.5859925645369017 2 1 0.02119453243098235 2 2 0.08547404639929113 2 3 0.06449805604206915 2 4 0.05375343716760739 2 5 0.049632095478926885 2 6 0.1645991368980333 2 7 -0.05819496276357938 2 8 0.024958971532598184 2 9 0.022201477858985186 2 10 -0.20729720710963306 2 11 0.033417163678052475 2 12 -0.5793354674774633 2 13 0.3018147239471034 2 14 0.029642437843016012 2 15 0.0353934469714261 2 16 -0.22176776972755619 2 17 -0.12405842100461939 2 18 -0.12034741333413024 2 19 -0.2893230707238956 2 20 -0.7369420926433747 2 21 -0.14397991328023352 2 22 -0.3744751665652615 2 23 -0.03136152508187706 2 24 -0.0700392221558559 2 25 0.17867396329540242 2 26 1.3325208096635848 2 27 0.7030224658893331 2 28 0.6391757776630769 2 29 0.7944935994120428 2 30 0.96937406958601 2 31 2.3076017977901557 2 32 2.5410336683381445 2 33 2.321547076928333 2 34 1.5213380328931834 2 35 2.060588718240858 2 36 1.3600373393998737 2 37 1.3895616128769746 2 38 1.816915361613944 2 39 1.1645658535871632 2 40 1.046911044288315 2 41 0.9931004182963292 2 42 1.4218734526014956 2 43 1.5628237930926474 2 44 1.2325574508365236 2 45 1.7806917539600846 2 46 1.3651123051035086 2 47 1.3725324317067198 2 48 -0.013773074325975188 2 49 0.8596093718900697 2 50 0.1431648455099328 2 51 0.8717450119305106 2 52 0.5739227236904085 2 53 0.37989016479470195 2 54 0.3513445828548747 2 55 0.8077045065845887 2 56 1.1765722746818312 3 1 -0.05947922643427006 3 2 0.3350307839879463 3 3 0.06361153121937235 3 4 -0.034455782690542947 3 5 0.39714982597537585 3 6 -0.21616327444948524 3 7 0.1137908528392586 3 8 0.19694478713543617 3 9 0.1990631799866449 3 10 0.4336602396988809 3 11 -0.4883026944779729 3 12 -0.21807660222923175 3 13 0.29117642607476313 3 14 -0.3599852217314492 3 15 -0.5005108083477197 3 16 -0.5147642236282621 3 17 -0.5420548749053253 3 18 -0.1172445764547021 3 19 0.305978347715822 3 20 0.08530968040626874 3 21 -0.825717501932715 3 22 0.2726879540021159 3 23 -0.12090053217407615 3 24 0.05052815373067432 3 25 0.406954105139377 3 26 0.4225030791671358 3 27 0.3382175013503286 3 28 0.3089452812091764 3 29 0.2745467909014039 3 30 0.4627251334157947 3 31 0.9250663367972436 3 32 1.331813810182119 3 33 0.856121545013437 3 34 1.377721011616586 3 35 0.9635142501557539 3 36 0.5045408854991678 3 37 1.1918665774159791 3 38 1.4100004679969231 3 39 0.6570303925942511 3 40 0.6386663634372525 3 41 1.0471784324807274 3 42 0.6944798355802178 3 43 0.13463230373094603 3 44 0.4293659614748222 3 45 0.0049825341019307245 3 46 -0.28249407787521363 3 47 -0.8743647314138485 3 48 0.19323047177331532 3 49 -0.856259422436171 3 50 -0.935735863709926 3 51 -0.5896911582822568 3 52 -0.877318411061367 3 53 -1.657343877758489 3 54 -2.571971019982005 3 55 -2.113838046606901 3 56 -0.26137098772951717 4 1 0.05775915828132128 4 2 -0.011039559042885116 4 3 0.10616472270873345 4 4 0.15877044397033302 4 5 0.2469110070435505 4 6 0.12165696002728765 4 7 -0.01907931650507777 4 8 0.023258291260489727 4 9 0.1178647431651072 4 10 -0.10780741119126702 4 11 -0.8539127682947338 4 12 -1.2115633586339243 4 13 -0.361450582175344 4 14 -0.9435718478712687 4 15 -0.3893514509877605 4 16 -0.8182813751697324 4 17 -0.3557761080794464 4 18 -1.531486869116442 4 19 -1.0644081047374954 4 20 -1.0154284872011985 4 21 -0.34721120579724385 4 22 -0.056447955680901885 4 23 -0.4964975795036466 4 24 -0.1652773173939508 4 25 0.14338484764914483 4 26 0.28702761238467644 4 27 0.05293743187573341 4 28 1.0783764579351853 4 29 -0.5027002781389811 4 30 -0.05486062429154259 4 31 0.22554397466090137 4 32 -1.482775855471374 4 33 -0.19120802511248058 4 34 0.026440073709508738 4 35 0.27614994273065463 4 36 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0.513534891214988 6 46 1.1163377952995859 6 47 0.6237579219027971 6 48 1.1982367295955925 6 49 0.6426976071841857 6 50 1.1260079827648326 6 51 1.5788528550677654 6 52 1.4881384099649182 6 53 1.6396940863633311 6 54 1.4530602691293844 6 55 1.9829496046238049 6 56 -0.024408117475033464 7 1 -0.16713880090235023 7 2 -0.5236926202673757 7 3 -0.4338352772912657 7 4 -0.42041322949905435 7 5 -0.6037012378544091 7 6 -0.37956752976862873 7 7 -0.5015282960969145 7 8 -0.4583743618007361 7 9 -1.2102985221410165 7 10 -0.13229720710963022 7 11 -0.9257495029886087 7 12 -0.15850213414412906 7 13 -0.21818527605289972 7 14 -0.0778575621569857 7 15 -0.39710655302857134 7 16 -0.2109344363942185 7 17 -0.17822508767128653 7 18 -0.18618074666746764 7 19 -0.12848973739055936 7 20 -0.32277542597670816 7 21 -1.022313246613571 7 22 -0.4111418332319232 7 23 -0.48302819174853795 7 24 -0.17670588882251792 7 25 -0.6104927033712642 7 26 -0.41664585700308265 7 27 -1.131144200777328 7 28 -0.3399908890035874 7 29 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120 4 -0.8004132294990569 120 5 -0.6837012378544074 120 6 -0.21956752976863214 120 7 -0.4015282960969131 120 8 -0.39837436180074093 120 9 -1.090298522141012 120 10 -0.5322972071096359 120 11 -1.265749502988612 120 12 -2.2185021341441242 120 13 -0.9181852760528955 120 14 -1.3778575621569829 120 15 -1.2371065530285748 120 16 -1.9709344363942236 120 17 -1.7582250876712848 120 18 -1.7461807466674628 120 19 -2.76848973739056 120 20 -1.8827754259767104 120 21 -2.602313246613569 120 22 -2.611141833231926 120 23 -1.9230281917485428 120 24 -2.896705888822517 120 25 -2.1904927033712625 120 26 -3.416645857003079 120 27 -2.1711442007773343 120 28 -1.3799908890035866 120 29 -1.8680064005879586 120 30 -2.37812593041399 120 31 -3.21323153554318 120 32 -2.0256329983285184 120 33 -2.1634529230716666 120 34 -2.548661967106817 120 35 -2.1769112817591427 120 36 -2.031629327266792 120 37 -2.618771720456362 120 38 -2.0380846383860565 120 39 -3.3012674797461727 120 40 -4.165588955711684 120 41 -3.468566248370337 120 42 -2.5906265473985037 120 43 -3.052176206907351 120 44 -2.8374425491634767 120 45 -1.6009749127065795 120 46 -2.6973876948964914 120 47 -2.183300901626616 120 48 -1.8829397409926436 120 49 -2.0145572947765977 120 50 -2.2285018211567333 120 51 -1.08075498806949 120 52 -1.9777439429762609 120 53 -1.4301098352052968 120 54 -1.1728220838117949 120 55 -1.559795493415411 120 56 -0.8800943919848372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 118.0 5 15.0 6 22.0 7 40.0 8 142.0 9 99.0 10 83.0 11 125.0 12 115.0 13 146.0 14 191.0 15 208.0 16 234.0 17 296.0 18 381.0 19 428.0 20 439.0 21 509.0 22 607.0 23 558.0 24 725.0 25 729.0 26 777.0 27 839.0 28 989.0 29 1156.0 30 1432.0 31 1733.0 32 1977.0 33 2378.0 34 2912.0 35 3598.0 36 4555.0 37 7370.0 38 8698.0 39 7536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 98.77684049079754 0.7112730061349692 0.31058282208588955 0.2013036809815951 8 97.55873046313165 1.3481637740914756 0.7690094927605715 0.3240962700163007 9 93.9167944785276 3.5678680981595092 1.3803680981595092 1.1349693251533743 10 43.96664110429448 39.36541411042945 6.7101226993865035 9.95782208588957 11 21.242331288343557 42.39647239263803 15.724693251533742 20.636503067484664 12 43.96280674846626 20.500383435582823 26.5260736196319 9.010736196319018 13 32.4079754601227 18.61196319018405 26.543328220858896 22.436733128834355 14 27.70897239263804 28.38381901840491 17.81441717791411 26.092791411042942 15 29.33282208588957 25.0920245398773 24.07400306748466 21.501150306748464 16 30.243481595092025 32.27185582822086 22.302530674846626 15.18213190184049 17 34.670245398773005 23.08473926380368 27.71088957055215 14.534125766871163 18 39.024156441717786 23.539110429447852 23.397239263803684 14.039493865030675 19 36.57592024539878 23.6579754601227 22.82975460122699 16.936349693251536 20 30.21146877815909 27.690331486416536 18.363082114304337 23.735117621120036 21 30.709355828220858 23.20168711656442 29.281058282208587 16.807898773006137 22 29.8715490797546 29.468941717791413 17.045628834355828 23.613880368098158 23 22.19516871165644 35.53680981595092 19.86196319018405 22.406058282208587 24 23.471441992445886 36.691144045861535 26.684816994842496 13.152596966850089 25 30.463461870338055 29.383904431362772 22.122298709516595 18.030334988782574 26 29.078667203775105 29.79609061786653 26.268439124513243 14.856803053845121 27 32.235415987262364 30.507011452358572 19.04697960827946 18.2105929520996 28 32.5069557708913 36.44248297035402 18.63379065528159 12.416770603473088 29 34.95257478591452 33.00756499366384 19.928190161668137 12.111670058753504 30 25.73844527721726 41.78341500912847 21.3048909387912 11.173248774863072 31 22.713552381135486 44.28892776805798 22.190606194605195 10.806913656201335 32 15.513562352217459 45.94859570541533 27.68988254291701 10.8479593994502 33 16.924053310767928 46.78731465276843 23.52443410196742 12.764197934496222 34 15.277350361594092 48.55362363440529 24.55570087705801 11.613325126942607 35 13.812611849827775 52.32743856678277 24.015240441049126 9.844709142340331 36 15.052645762352986 48.25508652383977 24.82916594483263 11.86310176897461 37 14.15125209323812 47.823994764498686 27.465208938848573 10.559544203414625 38 15.2314565200647 47.35423245782947 27.33959793576215 10.07471308634368 39 13.910665100063563 47.11944064564593 27.073983473621354 11.895910780669144 40 14.68722891566265 46.87614457831326 28.21783132530121 10.218795180722893 41 14.49892979039318 47.276268343007 27.16982587400451 11.054975992595306 42 13.110138275509614 47.096600003857056 27.44296376294525 12.35029795768808 43 13.654517741033011 47.62584653379382 28.040286326188042 10.679349398985124 44 13.87147184226265 47.083904076553544 28.437481913066964 10.607142168116837 45 13.263848677861418 47.95985331017178 28.204979733642155 10.571318278324648 46 13.930674905860771 47.78603842811625 27.30134208747707 10.981944578545912 47 14.112147727492225 47.959282223638716 27.640957292692818 10.287612756176237 48 15.278019365686788 47.672058908795734 26.854911965365957 10.195009760151525 49 14.541028266707883 47.84473597774214 26.904573294433604 10.70966246111637 50 13.172089785975292 48.37886433501537 27.533205730526074 10.915840148483266 51 13.010204081632654 48.74574829931973 28.05735930735931 10.186688311688313 52 13.574949260655261 47.62153281144293 27.439837634096843 11.363680293804968 53 13.533732843611057 47.42509182292673 26.879953605258073 12.161221728204136 54 13.741260784046428 47.82176868098697 26.689906327289727 11.747064207676877 55 14.253389289651306 47.52548010907614 26.08931092501982 12.131819676252732 56 14.25339366515837 47.944463781567855 25.830529450438956 11.971613102834821 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 3.5 7 7.0 8 16.0 9 25.0 10 163.5 11 302.0 12 1122.5 13 1219.0 14 495.0 15 381.5 16 268.0 17 235.0 18 202.0 19 269.5 20 337.0 21 412.5 22 551.0 23 614.0 24 678.0 25 742.0 26 1135.5 27 1529.0 28 2215.0 29 2531.5 30 2162.0 31 2291.0 32 2420.0 33 2643.0 34 2866.0 35 3351.5 36 3837.0 37 4387.0 38 3385.5 39 1834.0 40 1543.5 41 1253.0 42 1280.5 43 1308.0 44 1263.0 45 1218.0 46 1226.0 47 1243.5 48 1253.0 49 1257.5 50 1262.0 51 1281.5 52 1301.0 53 1331.5 54 1542.5 55 1723.0 56 1728.0 57 1733.0 58 1798.5 59 1864.0 60 1885.5 61 1907.0 62 1845.0 63 1569.5 64 1356.0 65 1238.0 66 1120.0 67 1001.0 68 882.0 69 742.0 70 602.0 71 515.5 72 328.5 73 228.0 74 196.5 75 165.0 76 145.0 77 125.0 78 93.5 79 50.5 80 39.0 81 29.5 82 20.0 83 18.0 84 16.0 85 10.5 86 5.0 87 3.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.028757668711656442 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0019171779141104297 21 0.0 22 0.0 23 0.0 24 0.005751533742331288 25 0.017254601226993866 26 0.05559815950920246 27 0.05943251533742332 28 0.08627300613496933 29 0.1495398773006135 30 0.23964723926380369 31 0.28182515337423314 32 0.2703220858895706 33 0.3125 34 0.3220858895705522 35 0.3700153374233129 36 0.4006901840490798 37 0.39685582822085885 38 0.4371165644171779 39 0.46587423312883436 40 0.5463957055214723 41 0.5770705521472392 42 0.5885736196319018 43 0.6345858895705522 44 0.6269171779141105 45 0.6710122699386504 46 0.718941717791411 47 0.745782208588957 48 0.80329754601227 49 0.7726226993865031 50 0.8378067484662577 51 0.7975460122699387 52 0.8148006134969324 53 0.8243865030674846 54 1.5548312883435584 55 0.8684815950920246 56 0.8550613496932514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 52160.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.29754601226993 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81294964028777 42.00536809815951 2 11.982014388489208 12.77223926380368 3 4.1079136690647475 6.56825153374233 4 1.8345323741007193 3.9110429447852764 5 0.8237410071942446 2.195168711656442 6 0.6402877697841727 2.0475460122699385 7 0.39208633093525175 1.4628067484662577 8 0.18705035971223022 0.7975460122699387 9 0.19784172661870503 0.9490030674846627 >10 0.8812949640287769 8.253450920245399 >50 0.0683453237410072 2.5479294478527605 >100 0.0539568345323741 5.341257668711656 >500 0.007194244604316546 3.199769938650307 >1k 0.01079136690647482 7.94861963190184 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1607 3.08090490797546 No Hit GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 1438 2.7569018404907975 No Hit GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1101 2.1108128834355826 No Hit GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 938 1.7983128834355828 No Hit GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 731 1.401457055214724 No Hit GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 284 0.5444785276073619 No Hit GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 276 0.5291411042944785 No Hit GCTGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 275 0.5272239263803681 No Hit GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 269 0.5157208588957055 No Hit GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 188 0.36042944785276076 No Hit GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 187 0.3585122699386503 No Hit GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 185 0.3546779141104294 No Hit GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 163 0.3125 No Hit GCTGTGGGGGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 161 0.30866564417177916 No Hit GCTGTGGGGGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 153 0.2933282208588957 No Hit GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 143 0.2741564417177914 No Hit GCTGTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 141 0.2703220858895706 No Hit GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 140 0.2684049079754601 No Hit GCTGTGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.2147239263803681 No Hit GCTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.20897239263803682 No Hit GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.19171779141104295 No Hit GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.19171779141104295 No Hit GCTGTGGGGGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAA 82 0.15720858895705522 No Hit GCTGTGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAA 80 0.15337423312883436 No Hit GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.14570552147239263 No Hit GCTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 76 0.14570552147239263 No Hit GCTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74 0.1418711656441718 No Hit GCTGTGGGGGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 74 0.1418711656441718 No Hit GCTGTGGGGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.13995398773006135 No Hit GCTGTGGGGGCTGCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA 65 0.12461656441717792 No Hit GCTGTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 63 0.12078220858895705 No Hit GCTGTGGGGAGTTCTATGGAGGTAAAATGTATTGGCTATAGAAGACAATAAGGGAG 62 0.11886503067484663 No Hit GCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 62 0.11886503067484663 No Hit GCTGTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 59 0.11311349693251534 No Hit GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.11311349693251534 No Hit GCTGTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 59 0.11311349693251534 No Hit GCTGTGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.10927914110429447 No Hit GCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACCCTGGAGCACTC 55 0.10544478527607362 No Hit GCTGTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.10161042944785276 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0019171779141104294 0.0 0.0 0.0 0.0 8 0.0019171779141104294 0.0 0.0 0.0 0.0 9 0.003834355828220859 0.0 0.0 0.0 0.0 10 0.023006134969325152 0.0 0.0 0.0 0.0 11 0.04409509202453988 0.0 0.0 0.0 0.0 12 0.04792944785276074 0.0 0.0 0.0 0.0 13 0.04792944785276074 0.0 0.0 0.0 0.0 14 0.09010736196319018 0.0 0.0 0.0 0.0 15 0.10352760736196319 0.0 0.0 0.0 0.0 16 0.10927914110429447 0.0 0.0 0.0 0.0 17 0.11311349693251534 0.0 0.0 0.0 0.0 18 0.12269938650306748 0.0 0.0 0.0 0.0 19 0.1303680981595092 0.0 0.0 0.0 0.0 20 0.15720858895705522 0.0 0.0 0.0 0.0 21 0.17254601226993865 0.0 0.0 0.0 0.0 22 0.19938650306748465 0.0 0.0 0.0 0.0 23 0.2338957055214724 0.0 0.0 0.0 0.0 24 0.24923312883435583 0.0 0.0 0.0 0.0 25 0.2741564417177914 0.0 0.0 0.0 0.0 26 0.30099693251533743 0.0 0.0 0.0 0.0 27 0.3182515337423313 0.0 0.0 0.0 0.0 28 0.40644171779141103 0.0 0.0 0.0 0.0 29 0.4217791411042945 0.0 0.0 0.0 0.0 30 0.4562883435582822 0.0 0.0 0.0 0.0 31 0.4677914110429448 0.0 0.0 0.0 0.0 32 0.4812116564417178 0.0 0.0 0.0 0.0 33 0.5061349693251533 0.0 0.0 0.0 0.0 34 0.5195552147239264 0.0 0.0 0.0 0.0 35 0.6077453987730062 0.0 0.0 0.0 0.0 36 0.6345858895705522 0.0 0.0 0.0 0.0 37 0.6499233128834356 0.0 0.0 0.0 0.0 38 0.6767638036809815 0.0 0.0 0.0 0.0 39 0.6940184049079755 0.0 0.0 0.0 0.0 40 0.7151073619631901 0.0 0.0 0.0 0.0 41 0.7381134969325154 0.0 0.0 0.0 0.0 42 0.7611196319018405 0.0 0.0 0.0 0.0 43 0.7841257668711656 0.0 0.0 0.0 0.0 44 0.8243865030674846 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAAGAT 15 0.00830767 50.118725 36 TCCGACT 15 0.008339454 50.070396 30 CTCCGAC 15 0.008339454 50.070396 29 GCCGGGT 15 0.00837133 50.02216 28 GGGCATC 15 0.008532065 49.78236 7 ATCTCAT 40 2.7011993E-9 49.78236 12 GGACTGG 15 0.008532065 49.78236 8 GAAGCTA 15 0.008532065 49.78236 9 GAAGTGG 40 2.7011993E-9 49.78236 9 CATCTCA 40 2.7011993E-9 49.78236 11 GGATCTA 15 0.008532065 49.78236 8 GGACATC 15 0.008532065 49.78236 8 CCATCTC 40 2.7011993E-9 49.78236 10 GGAAGTA 15 0.008532065 49.78236 8 AAGCGTG 15 0.008532065 49.78236 10 GGAAGGT 15 0.008532065 49.78236 8 GGGACTA 20 4.2371897E-4 49.78236 7 GGGACGC 35 5.340371E-8 49.78236 7 GGGATGT 15 0.008532065 49.78236 7 GCCATCT 40 2.7011993E-9 49.78236 9 >>END_MODULE