FastQCFastQC Report
Fri 17 Jun 2016
SRR1526922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304521
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT45561.496120136213923No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6340.20819582229140188No Hit
TCGGGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT4770.15663944358517146No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4270.14022021469783694No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4150.13627959976487666No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3910.12839836989895606No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTGTGGTTGTC3710.12183067834402225No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3150.10344114199020757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACTC201.9995077E-458.0497751
ATAGGGC258.686398E-657.96379529
CGATACT150.004707759257.9256726
CTTAAAT150.004716962657.8971113
ACGCATT150.004716962657.8971117
ATGCAAG407.2941475E-1057.88759623
CGCATTT150.00473540957.8400718
ACTGGTA150.00473540957.8400710
TCGGGTG303900.057.477431
CGGGTGG302000.057.43382
GGGTGGG298250.056.9674873
GGTGGGG199900.056.3340644
GTGGGGG99950.055.8399055
GGTGGGA54600.055.606274
TGGGTAC12650.055.5808266
GGTGGGT27850.055.3948554
GTGGGGA52100.055.318975
ACCTGGT11900.055.2211911
GTGGGAT12600.054.8790475
GTTGATC11750.054.44791816
Produced by FastQC (version 0.11.5)