FastQCFastQC Report
Fri 17 Jun 2016
SRR1526921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447601
Sequences flagged as poor quality0
Sequence length64
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT116762.6085732605601866No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC20620.4606781486189709No Hit
TCGGGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG17220.3847176391473656No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC17020.38024937388433No Hit
TCGGGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT15650.3496417568325361No Hit
TCGGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG11510.2571486658876991No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC11400.2546911199930295No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC10600.2368180589408871No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT10560.23592440588827995No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC9090.20308265620496826No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC8290.18520959515282584No Hit
TCGGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC8240.18409252883706692No Hit
TCGGGTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG7750.1731452789426297No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC7670.17135797283741547No Hit
TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC7280.16264485557449604No Hit
TCGGGTGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG6960.15549563115363907No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6730.15035712610114813No Hit
TCGGGTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG6600.14745275368017496No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC6430.1436547282065947No Hit
TCGGGTGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG6320.14119718231192513No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA6280.14030352925931802No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTGTGGTTGTC5600.12511142736499695No Hit
TCGGGTGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC5320.1188558559967471No Hit
TCGGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG5260.11751537641783642No Hit
TCGGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA5220.1166217233652293No Hit
TCGGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4630.10344034083927427No Hit
TCGGGTGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCAC4630.10344034083927427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGG201.976554E-458.1915353
CCGTTAT150.004653475258.100140
GCGTATG202.0030545E-458.03497332
CTAACCG150.004684567558.00246428
CGATACT453.092282E-1157.95701226
CTACTTA150.00473882757.83414
TCGGGTG447850.057.375571
CGGGTGG445600.057.320562
GGGTGGG442100.056.9624753
GGTGGGG302150.056.7617574
GTGGGGG144600.056.370835
GTGGGGA76700.055.8920175
GGTGGGA77050.055.6381344
GTGGGGC52950.055.377535
TGGGTAC16550.055.2686926
GTGGGAT15750.055.1354375
GTGGGGT32400.054.943995
TGGGGGT25000.054.6502466
TGGGGCT26850.054.5501986
GTGGGAA14300.054.248645
Produced by FastQC (version 0.11.5)