FastQCFastQC Report
Fri 17 Jun 2016
SRR1526920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445500
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT108092.426262626262626No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC18550.41638608305274977No Hit
TCGGGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG16170.362962962962963No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC15930.3575757575757576No Hit
TCGGGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT14520.3259259259259259No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC10950.2457912457912458No Hit
TCGGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG10910.24489337822671156No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT10370.23277216610549942No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC9070.20359147025813693No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC8180.18361391694725027No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC7740.17373737373737375No Hit
TCGGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC7550.16947250280583614No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC7180.1611672278338945No Hit
TCGGGTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG7040.1580246913580247No Hit
TCGGGTGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG6970.15645342312008978No Hit
TCGGGTGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG6410.14388327721661054No Hit
TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC6370.14298540965207632No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC6350.14253647586980922No Hit
TCGGGTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG5930.13310886644219977No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA5790.12996632996632998No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT5670.1272727272727273No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTGTGGTTGTC5480.12300785634118967No Hit
TCGGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4890.10976430976430977No Hit
TCGGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA4620.1037037037037037No Hit
TCGGGTGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC4620.1037037037037037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAGC150.004567059758.37573623
ATCTCTA150.00468933957.9874948
CGTTAAG150.004718709357.8961232
CCGATAA150.004733445657.8505427
TTATCCG150.00476937657.7401518
CGTACTT258.90039E-657.73367312
CGGGTGG442050.057.2880062
TCGGGTG446200.057.2418981
GGGTGGG437600.056.7867773
GGTGGGG304050.056.6581424
GTGGGGG148850.056.3221365
TGGGTAC16550.055.9154366
GGTGGGA71900.055.786274
GTGGGGA76200.055.712275
GTGGGGC54100.055.5924535
GTAGCAT14950.055.16663729
TAGCATA14800.055.14537430
CCAGTAG14950.055.10048326
GCCAGTA15000.054.88565425
GCATATG14750.054.7559932
Produced by FastQC (version 0.11.5)