FastQCFastQC Report
Fri 17 Jun 2016
SRR1526919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407746
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG123933.0393921706160207No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC36280.8897696114738097No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC25160.6170508110441304No Hit
TCGGGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC14100.34580351493331635No Hit
TCGGGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC12870.31563767639658025No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT12590.3087706562418761No Hit
TCGGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC9480.2324976823806978No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC9400.2305356766222109No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC8000.1962005758486901No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC6730.16505373443271054No Hit
TCGGGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC6550.16063922147611503No Hit
TCGGGTGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT6540.16039397075630418No Hit
TCGGGTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT6430.1576962128383847No Hit
TCGGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC6360.15597945779970865No Hit
TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG6240.15303644916197828No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC6010.14739568260632843No Hit
TCGGGTGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC5540.1358688987752179No Hit
TCGGGTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC5500.13488789589597444No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTG5010.12287061062524218No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT4980.1221348584658096No Hit
TCGGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG4750.11649409191015975No Hit
TCGGGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4520.11085332535450991No Hit
TCGGGTGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGG4390.10766506599696869No Hit
TCGGGTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC4110.10079804584226454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGG150.00822220250.31229847
ATGACAC150.00823026450.29982445
AGTACGT150.00831121150.1754438
ATGCTAG150.0083315450.14444436
TTACACA150.00838048150.070233
CGACATT150.0084255350.0023429
GCGCTTA150.0084255350.0023429
CGAAACT150.00849550749.8978225
CAATAAT150.00849550749.8978225
ATACATC204.2436217E-449.84879323
TACGTTT150.00854930849.8181922
GTATATC204.259077E-449.8120821
CTACGTT150.00855345749.81207721
GTCGTAC150.00858254449.76931410
GGCGAAT150.00858254449.7693149
CGCTTAG204.277165E-449.76931414
TCGGGTG407950.049.3850171
CGGGTGG405550.049.315162
GGGTGGG401600.048.95743
GGTGGGG278000.048.826464
Produced by FastQC (version 0.11.5)