##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526919_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 407746 Sequences flagged as poor quality 0 Sequence length 56 %GC 59 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.536664982611725 38.0 38.0 38.0 35.0 40.0 2 37.38905102686476 38.0 38.0 38.0 35.0 40.0 3 37.25803073482021 38.0 38.0 38.0 35.0 40.0 4 37.1766148533646 38.0 38.0 38.0 34.0 40.0 5 37.208823630397355 38.0 38.0 38.0 34.0 40.0 6 35.396899049898714 36.0 34.0 39.0 30.0 39.0 7 38.31996389909404 39.0 38.0 40.0 37.0 40.0 8 37.51991190594144 39.0 38.0 40.0 35.0 40.0 9 37.522013704610224 39.0 37.0 40.0 35.0 40.0 10 36.90509287644759 38.0 35.0 39.0 32.0 40.0 11 36.63889284015048 38.0 35.0 39.0 31.0 40.0 12 36.82790021238712 38.0 35.0 39.0 32.0 40.0 13 36.80057682969299 38.0 35.0 39.0 32.0 40.0 14 37.11315622961354 38.0 36.0 39.0 33.0 40.0 15 37.13327169365242 38.0 36.0 39.0 33.0 40.0 16 37.506388781251076 39.0 38.0 39.0 33.0 40.0 17 37.32906024829183 39.0 37.0 39.0 33.0 40.0 18 37.24712934032461 39.0 37.0 39.0 33.0 40.0 19 37.164761886076136 39.0 36.0 39.0 33.0 40.0 20 37.068756039298975 38.0 36.0 39.0 33.0 40.0 21 36.894547095495724 38.0 36.0 39.0 33.0 40.0 22 36.77668450456902 38.0 36.0 39.0 33.0 40.0 23 36.661063505221385 38.0 36.0 39.0 32.0 40.0 24 36.54205068841877 38.0 36.0 39.0 31.0 40.0 25 36.32623005498521 38.0 35.0 39.0 31.0 40.0 26 35.86453331240527 38.0 35.0 39.0 30.0 40.0 27 35.61873323098203 38.0 35.0 39.0 29.0 40.0 28 35.31424710481525 38.0 35.0 39.0 29.0 40.0 29 35.161247443261246 38.0 35.0 39.0 29.0 40.0 30 34.80076322024005 38.0 34.0 39.0 28.0 40.0 31 34.02846134603405 37.0 33.0 39.0 26.0 40.0 32 33.854988154390234 37.0 33.0 39.0 25.0 40.0 33 33.634578879989014 37.0 33.0 38.0 24.0 40.0 34 33.82218832312273 37.0 33.0 39.0 25.0 40.0 35 33.534526396334975 37.0 33.0 39.0 23.0 40.0 36 33.32902346068386 37.0 33.0 39.0 22.0 40.0 37 33.078906966591944 37.0 33.0 39.0 20.0 40.0 38 32.856646049256156 36.0 32.0 39.0 18.0 40.0 39 32.65012532311783 36.0 32.0 39.0 17.0 40.0 40 32.3019968313607 36.0 31.0 39.0 14.0 40.0 41 31.84489363476282 36.0 31.0 39.0 4.0 40.0 42 31.57562305945368 36.0 30.0 39.0 4.0 40.0 43 31.381529187288166 36.0 30.0 39.0 4.0 40.0 44 31.155891167540577 36.0 30.0 39.0 4.0 40.0 45 30.732061136099436 35.0 30.0 38.0 4.0 39.0 46 29.992110284343685 35.0 29.0 38.0 4.0 39.0 47 29.594777140670907 35.0 28.0 38.0 4.0 39.0 48 29.344601786406244 35.0 27.0 38.0 4.0 39.0 49 29.067841254114082 34.0 27.0 38.0 4.0 39.0 50 28.63960161473074 34.0 26.0 38.0 4.0 39.0 51 27.545440053366555 33.0 23.0 38.0 4.0 39.0 52 27.296108361578042 33.0 20.0 38.0 4.0 39.0 53 26.956610242651063 33.0 17.0 38.0 4.0 39.0 54 26.46957664820746 33.0 4.0 37.0 4.0 39.0 55 26.119395407925524 33.0 4.0 37.0 4.0 39.0 56 15.679143388285844 19.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5804817711569541 1 2 -0.8985419239406269 1 3 -1.0200650773507576 1 4 -0.8466591651906654 1 5 -1.4180552438405343 1 6 -0.7909175427627488 1 7 -0.6607230566084894 1 8 -1.3520645460077603 1 9 -1.3411620682671455 1 10 -1.1466936442509734 1 11 -1.2916781002762576 1 12 -1.166613139090778 1 13 -0.6592230422910674 1 14 -1.0793281240339567 1 15 -1.0841208605915185 1 16 -0.38101349313406985 1 17 -0.7879414850930218 1 18 -0.56525655961741 1 19 -0.6268206639868268 1 20 -0.6976907291490235 1 21 -0.6153661320441373 1 22 -0.8700374591236368 1 23 -0.531127315608984 1 24 -0.5852258835306685 1 25 -0.8415455554884659 1 26 -0.6143830571277604 1 27 -1.1972870290410569 1 28 -0.6468640132081873 1 29 -0.8274417879344114 1 30 -1.167465001092232 1 31 -0.9733586831655927 1 32 -0.8465629180844445 1 33 -0.8771908950173284 1 34 -1.369805507301919 1 35 -1.6028416891458477 1 36 -0.04629830293139747 1 37 -0.15226673460657736 1 38 0.3933055078461365 1 39 0.3402137563502947 1 40 0.08732097048260101 1 41 0.8920789725045459 1 42 0.6933835912125623 1 43 0.8495136904960319 1 44 0.710639285229572 1 45 0.741671070821436 1 46 0.9793074437737772 1 47 1.3151005060366892 1 48 0.741204788670121 1 49 1.03505386561622 1 50 1.0013833142267998 1 51 1.426239632678378 1 52 1.4701807776191558 1 53 1.2027972499479382 1 54 1.2761200059153417 1 55 1.1850776631680873 1 56 1.0359728753268946 2 1 -0.03228123901860158 2 2 -0.08991568111748904 2 3 -0.0950265673381594 2 4 -0.03626846360825198 2 5 -0.015310530214989626 2 6 -0.3284052895769207 2 7 0.014350462506477868 2 8 -0.08439895295356337 2 9 -0.08689795959888613 2 10 -0.23697511743400668 2 11 -0.2855164982597387 2 12 -0.2195188945872104 2 13 -0.1567163905616198 2 14 -0.15535389073329497 2 15 0.13902715789328823 2 16 0.29844316559720596 2 17 -0.020415928448294096 2 18 -0.11702802019716074 2 19 -0.1274928387522678 2 20 0.25654537223734053 2 21 -0.33292669779122974 2 22 0.07992753177399692 2 23 0.15621339297524628 2 24 0.21223945745799 2 25 -0.3073066533739137 2 26 0.32856610965560407 2 27 0.40282500008651567 2 28 0.32208991481309823 2 29 0.10786139089208291 2 30 0.4939689717407063 2 31 0.3886634425871023 2 32 0.866880577224336 2 33 0.5393894158634964 2 34 0.38134979307617556 2 35 0.5187799324757734 2 36 0.38729643169960326 2 37 0.7501558952771958 2 38 0.9006714229840753 2 39 0.41747184345293675 2 40 -0.11468155017277581 2 41 0.9127203392599021 2 42 0.8080874142065362 2 43 1.1736139565652053 2 44 1.2765964340882796 2 45 1.181945542183989 2 46 1.4359241641210687 2 47 1.5258132913608726 2 48 1.1488507766269898 2 49 1.561982867156619 2 50 1.3889760883480697 2 51 1.753770790779484 2 52 2.3949672220947207 2 53 2.1354537406355156 2 54 2.2136357600114067 2 55 1.9706539129930434 2 56 1.556390183827105 3 1 0.017152504339911445 3 2 -0.09017936290630502 3 3 -0.12606191388194787 3 4 0.009678088093153292 3 5 -0.056800859685388616 3 6 -0.5202140148073084 3 7 0.12304009585695042 3 8 -0.06161684639975107 3 9 -0.010634594228221772 3 10 -0.246012810745718 3 11 -0.38857652541896215 3 12 0.07262075128814871 3 13 0.22138351846816562 3 14 -0.15184033089730775 3 15 0.13408971639768197 3 16 0.13174353870693523 3 17 -0.025999390326497007 3 18 0.24698468926506223 3 19 0.3938587940972127 3 20 0.07548167990125165 3 21 0.20214298012146514 3 22 0.2811694729993235 3 23 -0.04620193221031599 3 24 0.17859366120490705 3 25 0.10708246184184134 3 26 0.12408747447253887 3 27 0.46393984668963384 3 28 0.32313804992364936 3 29 0.6785412943845444 3 30 0.5273840464285726 3 31 0.3590256095240463 3 32 0.46274868359357413 3 33 0.28031546630582227 3 34 0.32560746292021037 3 35 0.6918079222976559 3 36 0.7292191993036639 3 37 0.6272669975989089 3 38 0.94664434286436 3 39 0.6460180339102948 3 40 0.40500888740715624 3 41 0.8833725561645416 3 42 1.1261667560567936 3 43 0.9101628893131704 3 44 1.092249903481413 3 45 1.1342850588552693 3 46 1.1976349315669133 3 47 1.4508683349342881 3 48 1.7286540700125315 3 49 1.44505317790561 3 50 1.5712065725996744 3 51 1.7215356920965732 3 52 1.8406092213300447 3 53 1.3620091335873 3 54 1.7861812121597573 3 55 2.040094512078067 3 56 0.9455251757188918 4 1 -0.07159130174017037 4 2 -0.2483285526746215 4 3 -0.2765373390861825 4 4 -0.1946677000413075 4 5 -0.3334179749927344 4 6 -0.40649364518095865 4 7 -0.03839019743352168 4 8 -0.10910579778585117 4 9 -0.19848781506657076 4 10 -0.30302365847573043 4 11 -0.4009241884697161 4 12 0.12677235166313494 4 13 -0.046776385107570206 4 14 -0.06410764039242167 4 15 -0.14343807254031304 4 16 -0.2838584433474409 4 17 -0.4417821678810725 4 18 -0.5438483092617901 4 19 -0.31408951178198663 4 20 -0.15963952004518234 4 21 -0.5245410964822597 4 22 -0.05936889582349636 4 23 -0.5470590368038657 4 24 0.005599152031209087 4 25 -0.17013730513284742 4 26 0.18952874941562214 4 27 -0.14458432633835372 4 28 -0.062297868115727795 4 29 0.5012210854653034 4 30 0.16134011981814922 4 31 0.7288461532497692 4 32 1.3751298272925112 4 33 0.49649331550898523 4 34 1.009710851161806 4 35 0.9740430903705075 4 36 0.900358880567893 4 37 1.1068371060762132 4 38 1.2170608421277862 4 39 1.0731440550700668 4 40 0.6922996333560008 4 41 1.496630892845939 4 42 1.4757448998896585 4 43 1.0684326094149483 4 44 1.5558028698668416 4 45 1.56329269244306 4 46 1.2971595063626822 4 47 1.5294675046142139 4 48 1.6585589849716875 4 49 1.5761633961994335 4 50 1.129690568849842 4 51 1.583849874499144 4 52 1.56157480322371 4 53 1.6722140351541945 4 54 1.956845468219754 4 55 1.7857889007214318 4 56 0.8351193902628857 5 1 -0.3799142745264703 5 2 -0.41143119116060234 5 3 -0.46548608891350085 5 4 -0.42820133728401544 5 5 -0.34094663917286994 5 6 -0.9129931616887603 5 7 -0.1125993423430458 5 8 -0.0996846070136499 5 9 -0.09696826816782789 5 10 -0.026341796340069834 5 11 -0.04460425477721941 5 12 -0.156547167607485 5 13 0.318736807322324 5 14 0.04603188164101368 5 15 -0.13292557081355483 5 16 -0.058675407016451686 5 17 0.2409157185673294 5 18 -0.5168349096209539 5 19 -0.04854869122666372 5 20 0.09786151600952309 5 21 0.21735004870354402 5 22 0.2327432743657596 5 23 0.4920762028701233 5 24 0.25352704262382986 5 25 0.22314906038725724 5 26 0.593583176822726 5 27 0.2531847025330336 5 28 0.6955208913814417 5 29 0.5782828343256412 5 30 0.7861847116125986 5 31 1.0104251006181926 5 32 0.5215934272372564 5 33 0.7745151417280738 5 34 0.897485405658287 5 35 0.5529006673839092 5 36 1.2516090532436763 5 37 0.886067662782942 5 38 0.6343284205225928 5 39 0.798389254683272 5 40 1.4116807633463253 5 41 1.440904963850226 5 42 0.8800687015197148 5 43 0.587067780018625 5 44 1.1447032704746611 5 45 0.9722165769574929 5 46 0.8030926462991381 5 47 0.8862433023763359 5 48 0.7400343268697398 5 49 0.9657199647862562 5 50 1.0807335305848902 5 51 1.7621479819725536 5 52 2.202733803085568 5 53 2.001527476237218 5 54 1.7539237939553516 5 55 2.1695850051557457 5 56 1.295928894338033 6 1 -0.46510382636724756 6 2 -0.5187760618218178 6 3 -0.4371340179491128 6 4 -0.5025342916540225 6 5 -0.4611973163943688 6 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0.8408049928778141 7 30 1.0674437296280175 7 31 0.947597875487304 7 32 1.1751436051927584 7 33 0.9725473248779579 7 34 1.0764234441626357 7 35 1.4219212937323178 7 36 1.4465699579996922 7 37 1.658600677307959 7 38 1.6645945979833954 7 39 1.8077954741937887 7 40 1.8210127774728306 7 41 1.628926291027259 7 42 1.4995531115182068 7 43 1.6317833947552671 7 44 1.3744994805033777 7 45 1.415648713385469 7 46 1.2409318954505864 7 47 1.1940307451763523 7 48 1.7111354523180609 7 49 1.2459071272548208 7 50 2.0660642255045687 7 51 2.300769136003691 7 52 2.1101694573984133 7 53 2.6458706898800166 7 54 2.2693420237446915 7 55 2.6037277268443297 7 56 2.0291948931562445 8 1 -0.5710530242282132 8 2 -0.6432747249234225 8 3 -0.8427155687512524 8 4 -1.5204153076968083 8 5 -1.0342253912606836 8 6 0.18253147443626716 8 7 -0.468324407959841 8 8 -0.9628139317571467 8 9 -1.116131355736428 8 10 0.03606126975393664 8 11 0.26890236655420807 8 12 0.2672627822851439 8 13 0.44670447613645337 8 14 0.009845708889876903 8 15 0.06921144899079223 8 16 -0.051621601242175075 8 17 -0.22025106740260014 8 18 0.16871149935064977 8 19 -0.07201415293031488 8 20 -0.2790175104758035 8 21 0.036553401125395624 8 22 0.02229976446358961 8 23 -0.2018736301052968 8 24 -0.00830635036113847 8 25 -0.11496386015677018 8 26 0.2706292278845197 8 27 0.044620219116190185 8 28 0.3285813184871458 8 29 0.2822019467093213 8 30 1.0083795935023616 8 31 0.8513341915272861 8 32 1.2800341335126078 8 33 1.2949681958917623 8 34 1.7035206597742487 8 35 1.8199162961121402 8 36 2.1613337609506686 8 37 2.3743610295211894 8 38 2.031235483276113 8 39 2.0677014713380117 8 40 2.3361692505808804 8 41 1.9781201272956999 8 42 2.253226957305923 8 43 1.8843417003240432 8 44 2.174014592354883 8 45 2.4485199651703304 8 46 2.032117640333972 8 47 2.2224100883462725 8 48 1.908050611765944 8 49 2.2642614823238354 8 50 2.5651271717206576 8 51 3.356337912776656 8 52 3.7579264778648565 8 53 3.563930542331228 8 54 3.1522103007556375 8 55 3.0234683265587528 8 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4 146.0 5 39.0 6 39.0 7 46.0 8 88.0 9 153.0 10 134.0 11 266.0 12 390.0 13 598.0 14 958.0 15 1296.0 16 1608.0 17 2196.0 18 2837.0 19 2985.0 20 3702.0 21 4225.0 22 4231.0 23 5068.0 24 6861.0 25 7232.0 26 6964.0 27 8411.0 28 9226.0 29 10403.0 30 12208.0 31 14399.0 32 17433.0 33 22393.0 34 28879.0 35 39403.0 36 54072.0 37 65868.0 38 51467.0 39 21522.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.35621686049649 0.4591093474859349 0.07504672026212397 0.10962707175545561 8 99.19849069935182 0.5232994607537672 0.14229427437280118 0.13591556552160663 9 98.03897524439235 0.9540253000642558 0.4282077567897662 0.5787916987536359 10 67.84174461552045 16.407763656786333 8.746621671334605 7.003870056358616 11 42.17797354235235 27.183344533116205 13.64378804451791 16.994893880013535 12 33.737179518621886 14.437419373825863 23.325795961211146 28.499605146341107 13 27.613269044944648 15.219523919302702 25.468551500198654 31.698655535554 14 24.88583578992804 16.303532100866715 32.8751232384867 25.935508870718536 15 30.084170047039088 17.78067718628754 27.81780814526691 24.317344621406463 16 36.08153114929392 17.35345043237702 22.52578811318811 24.039230305140947 17 32.15506712512201 17.810843024824276 26.12288042065403 23.911209429399673 18 30.891044915216824 18.49852604317394 26.55010717456456 24.060321867044678 19 35.221927376356845 20.206697306656597 20.071318909320997 24.500056407665557 20 31.406884204588653 23.932604936909097 21.117119768482752 23.543391090019497 21 30.28699239232267 20.09412722626341 26.58787578541543 23.03100459599849 22 28.76300441941797 17.970991744860772 25.977937245245812 27.28806659047544 23 30.439197143305602 18.96827421126982 22.71694985088683 27.87557879453775 24 31.60827634922348 18.638218760117336 24.441768289691844 25.31173660096734 25 33.2104380077711 18.799305310255505 20.14428549001138 27.84597119196201 26 26.646560376538954 17.90645912525368 23.67454312995551 31.772437368251854 27 25.494815632860913 17.30437450150316 23.62844346278913 33.5723664028468 28 26.970597838227967 23.592184627016994 22.040697089242446 27.39652044551259 29 33.35404809919507 20.27967390209694 20.44031568320655 25.925962315501437 30 28.907162260162 17.841611662180267 27.512813894317638 25.738412183340092 31 31.27112099285268 21.042288716286343 22.8822438549286 24.804346435932374 32 38.12947163119696 18.54886114055648 20.109151456869434 23.212515771377134 33 33.8188876615405 18.34470339035173 21.808614017521286 26.027794930586477 34 29.17761636204368 22.36198462613557 27.340036023267487 21.12036298855326 35 27.725382746320243 23.05069557789217 29.110630242067508 20.11329143372008 36 28.513769420386577 28.51795577729566 21.666859567426204 21.30141523489156 37 29.72694068650269 23.301537574902284 24.725081213410867 22.24644052518416 38 33.50014167621441 23.510120609577314 20.331891931847583 22.657845782360695 39 31.222361901944833 20.517636619093395 20.614508614952303 27.645492864009462 40 29.413520480530853 19.782675728011643 24.402481591573444 26.40132219988406 41 27.35630255418262 20.71449015040859 25.096719434684772 26.832487860724015 42 28.393142270493847 19.920188940467465 23.105721273324498 28.58094751571419 43 24.89556793268946 17.242154016314114 28.4820564476309 29.380221603365523 44 26.165658560671524 17.301320824589556 23.90964078508826 32.62337982965066 45 27.832862064281922 18.622248015113538 26.55562003778384 26.9892698828207 46 26.722645065822793 20.119120847630555 22.8303429603334 30.32789112621326 47 25.34480329963351 22.778394221193075 25.187877949146305 26.68892453002711 48 27.260185235549798 21.498400328340644 25.80712954126263 25.434284894846932 49 28.530642613663048 21.39013375322271 24.013288806602876 26.065934826511366 50 29.788102268848952 18.374362550884637 23.485826495130407 28.351708685135996 51 25.702594785704573 22.994325837835163 25.974311745144824 25.32876763131544 52 26.29571832393144 19.272364851329662 27.55724605602558 26.874670768713322 53 26.065601745027188 18.17316229296969 29.561344284269282 26.199891677733838 54 26.618122372490237 21.36023703969518 25.71501907631273 26.30662151150186 55 26.194505891493584 21.105700471616412 23.983154435817113 28.71663920107289 56 29.184602444213546 19.081193409529465 21.229528474593042 30.504675671663943 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.0 7 4.0 8 4.0 9 4.0 10 4.0 11 4.0 12 40.0 13 190.5 14 305.0 15 389.5 16 474.0 17 518.5 18 563.0 19 546.0 20 529.0 21 596.0 22 669.5 23 676.0 24 767.0 25 858.0 26 1043.0 27 1228.0 28 1574.0 29 2250.5 30 2581.0 31 2923.0 32 3265.0 33 3499.5 34 3734.0 35 3539.5 36 3345.0 37 3257.0 38 3572.0 39 3975.0 40 3998.5 41 4022.0 42 4544.0 43 5066.0 44 5258.5 45 5451.0 46 6873.0 47 15482.5 48 22670.0 49 16251.0 50 9832.0 51 10235.5 52 10639.0 53 12515.0 54 15388.0 55 16385.0 56 17430.0 57 18475.0 58 20796.5 59 23118.0 60 27767.5 61 32417.0 62 36179.5 63 42129.5 64 44317.0 65 37929.5 66 31542.0 67 27963.5 68 24385.0 69 22099.5 70 19814.0 71 17499.0 72 13518.0 73 11852.0 74 10123.0 75 8394.0 76 7011.5 77 5629.0 78 4568.5 79 2846.0 80 2184.0 81 1768.0 82 1352.0 83 1078.5 84 805.0 85 602.0 86 399.0 87 299.5 88 131.5 89 63.0 90 44.5 91 26.0 92 20.0 93 14.0 94 7.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03433510077352077 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 2.4525071981086263E-4 21 0.0 22 0.0 23 4.905014396217253E-4 24 0.00735752159432588 25 0.020110559024490736 26 0.06008642635366134 27 0.06646294506874378 28 0.09687403432529075 29 0.1542627027610326 30 0.235685941738239 31 0.2844908349806007 32 0.28326458138154637 33 0.3112231634399847 34 0.3291264659861777 35 0.3769503563492959 36 0.4083424484850863 37 0.42109548591525114 38 0.4637691111623413 39 0.5042354799311336 40 0.5790369494734467 41 0.6008642635366135 42 0.6226915775997803 43 0.6616864420497074 44 0.6611959406100857 45 0.6893997733883349 46 0.7227538712826123 47 0.7590509778146199 48 0.8063843667381163 49 0.7835760497957062 50 0.833852447356933 51 0.8046676116994402 52 0.8326261937578787 53 0.8331166951975003 54 1.5850554021376053 55 0.8838835941983489 56 0.8647540380531017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 407746.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.78441016448409 #Duplication Level Percentage of deduplicated Percentage of total 1 70.07850115149786 25.0771782891747 2 11.781922898405469 8.43218323045737 3 5.5675541826969726 5.976949274598524 4 3.3008650731054403 4.724780386944994 5 2.0944606538797963 3.7474519555904093 6 1.4290602252395828 3.0682846349833945 7 0.9556058084724968 2.3937053144160276 8 0.7562587421069924 2.1649818414426734 9 0.5796360618700878 1.8667741125676947 >10 3.0698655099618426 19.96969119861164 >50 0.22101698969747013 5.429441995412907 >100 0.15233170982225633 9.538452263556287 >500 0.008840679587898805 2.1363933161996287 >1k 0.0034002613799610786 2.457862227317035 >5k 0.0 0.0 >10k+ 6.800522759922157E-4 3.015869958726734 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCGGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 12393 3.0393921706160207 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 3628 0.8897696114738097 No Hit TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 2516 0.6170508110441304 No Hit TCGGGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 1410 0.34580351493331635 No Hit TCGGGTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 1287 0.31563767639658025 No Hit TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 1259 0.3087706562418761 No Hit TCGGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 948 0.2324976823806978 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 940 0.2305356766222109 No Hit TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 800 0.1962005758486901 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 673 0.16505373443271054 No Hit TCGGGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 655 0.16063922147611503 No Hit TCGGGTGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT 654 0.16039397075630418 No Hit TCGGGTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 643 0.1576962128383847 No Hit TCGGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 636 0.15597945779970865 No Hit TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 624 0.15303644916197828 No Hit TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 601 0.14739568260632843 No Hit TCGGGTGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC 554 0.1358688987752179 No Hit TCGGGTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 550 0.13488789589597444 No Hit TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTG 501 0.12287061062524218 No Hit TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT 498 0.1221348584658096 No Hit TCGGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 475 0.11649409191015975 No Hit TCGGGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 452 0.11085332535450991 No Hit TCGGGTGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGG 439 0.10766506599696869 No Hit TCGGGTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 411 0.10079804584226454 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0014715043188651758 0.0 0.0 0.0 0.0 9 0.0014715043188651758 0.0 0.0 0.0 0.0 10 0.0024525071981086263 0.0 0.0 0.0 0.0 11 0.006621769434893291 0.0 0.0 0.0 0.0 12 0.007112270874515016 0.0 0.0 0.0 0.0 13 0.008829025913191055 0.0 0.0 0.0 0.0 14 0.010055279512245368 0.0 0.0 0.0 0.0 15 0.01030053023205623 0.0 0.0 0.0 0.0 16 0.010545780951867093 0.0 0.0 0.0 0.0 17 0.012017285270732269 0.0 0.0 0.0 0.0 18 0.01299828814997572 0.0 0.0 0.0 0.0 19 0.01397929102921917 0.0 0.0 0.0 0.0 20 0.01569604606789521 0.0 0.0 0.0 0.0 21 0.017412801106571247 0.0 0.0 0.0 0.0 22 0.019374806865058148 0.0 0.0 0.0 0.0 23 0.02060106046411246 0.0 0.0 0.0 0.0 24 0.02158206334335591 0.0 0.0 0.0 0.0 25 0.0223178155027885 0.0 0.0 0.0 0.0 26 0.023544069101842813 0.0 0.0 0.0 0.0 27 0.024034570541464538 0.0 0.0 0.0 0.0 28 0.0262418270197623 0.0 0.0 0.0 0.0 29 0.027713331338627478 0.0 0.0 0.0 0.0 30 0.02967533709711438 0.0 0.0 0.0 0.0 31 0.03065633997635783 0.0 0.0 0.0 0.0 32 0.032618345734844734 0.0 0.0 0.0 0.0 33 0.034580351493331635 0.0 0.0 0.0 0.0 34 0.036542357251818536 0.0 0.0 0.0 0.0 35 0.03801386157068371 0.0 0.0 0.0 0.0 36 0.03997586732917061 0.0 0.0 0.0 0.0 37 0.04120212092822492 0.0 0.0 0.0 0.0 38 0.043899878846144415 0.0 0.0 0.0 0.0 39 0.045126132445198724 0.0 0.0 0.0 0.0 40 0.048069141082929076 0.0 0.0 0.0 0.0 41 0.0505216482810377 0.0 0.0 0.0 2.4525071981086263E-4 42 0.05297415547914633 0.0 0.0 0.0 2.4525071981086263E-4 43 0.054445659798011505 0.0 0.0 0.0 2.4525071981086263E-4 44 0.05567191339706582 0.0 0.0 0.0 2.4525071981086263E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGGG 15 0.008222202 50.312298 47 ATGACAC 15 0.008230264 50.299824 45 AGTACGT 15 0.008311211 50.17544 38 ATGCTAG 15 0.00833154 50.144444 36 TTACACA 15 0.008380481 50.0702 33 CGACATT 15 0.00842553 50.00234 29 GCGCTTA 15 0.00842553 50.00234 29 CGAAACT 15 0.008495507 49.89782 25 CAATAAT 15 0.008495507 49.89782 25 ATACATC 20 4.2436217E-4 49.848793 23 TACGTTT 15 0.008549308 49.81819 22 GTATATC 20 4.259077E-4 49.81208 21 CTACGTT 15 0.008553457 49.812077 21 GTCGTAC 15 0.008582544 49.769314 10 GGCGAAT 15 0.008582544 49.769314 9 CGCTTAG 20 4.277165E-4 49.769314 14 TCGGGTG 40795 0.0 49.385017 1 CGGGTGG 40555 0.0 49.31516 2 GGGTGGG 40160 0.0 48.9574 3 GGTGGGG 27800 0.0 48.82646 4 >>END_MODULE