FastQCFastQC Report
Fri 17 Jun 2016
SRR1526918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156941
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.31476797012890195No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3940.25104975755220116No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3410.2172791048865497No Hit
TGAAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.21281883000618065No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC2740.17458790246016018No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2270.1446403425491108No Hit
TGAAGCGGGACAGTGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.11469278263806144No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA1690.10768377925462434No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1580.10067477587118726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC150.00449803458.5826358
CGATTCA150.00465532158.07647751
AGTATAG150.00465532158.07647751
ATTCACG150.00465532158.07647753
GTATGGA303.7066457E-758.07647753
ATATTCG150.00465532158.07647755
TAGTAAA150.00465532158.07647755
CGGGTAT258.583835E-658.0579036
CGGGATA850.058.0579036
TCGATCC150.00466122458.057945
TTATACG303.7148675E-758.057945
AGCGGAT150.00466122458.05794
AAATTGT150.00466122458.057949
TATACGT303.7148675E-758.057946
CGTAGAG150.00467304758.0207839
AACCTAC150.00467304758.0207839
GCACTAA150.00467304758.0207839
TCACGTC407.1122486E-1058.0207839
CGTCACG351.6285412E-858.0022437
TGGAGTA150.004678966558.00223531
Produced by FastQC (version 0.11.5)