FastQCFastQC Report
Fri 17 Jun 2016
SRR1526917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235418
Sequences flagged as poor quality0
Sequence length64
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11950.5076077445225089No Hit
TGAAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG11150.47362563610259195No Hit
TGAAGCGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCA11000.4672539907738576No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC10430.4430417385246668No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9720.4128826173019905No Hit
TGAAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT7650.3249539117654555No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC7340.3117858447527377No Hit
TGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6790.28842314521404483No Hit
TGAAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG5910.25104282595213623No Hit
TGAAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA5820.24721983875489553No Hit
TGAAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC5670.24084819342616112No Hit
TGAAGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA5380.22852967912394126No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA5330.22640579734769642No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC5240.22258281015045578No Hit
TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT4740.2013439923880077No Hit
TGAAGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC4590.1949723470592733No Hit
TGAAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4110.17458308200732314No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4020.1707600948100825No Hit
TGAAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT3850.16353889677085015No Hit
TGAAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC3800.16141501499460534No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA3690.15674247508686676No Hit
TGAAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3610.15334426424487507No Hit
TGAAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC3310.14060097358740622No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG3300.14017619723215727No Hit
TGAAGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG3270.13890186816641037No Hit
TGAAGCGGGAGCTCGTATAAGTGCACCTGACACACCAGCGTCATCACAGAACTCCACAGAACAG3230.13720276274541454No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3110.132105446482427No Hit
TGAAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA3020.12828245928518633No Hit
TGAAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG2820.11978693218020711No Hit
TGAAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2680.11384006320672166No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2670.1134152868514727No Hit
TGAAGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2630.11171618143047686No Hit
TGAAGCGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC2520.10704364152273828No Hit
TGAAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC2510.10661886516748931No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG2490.10576931245699141No Hit
TGAAGCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT2470.10491975974649347No Hit
TGAAGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC2430.10322065432549762No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2430.10322065432549762No Hit
TGAAGCGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC2370.10067199619400385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGGA258.497529E-658.17221558
CGTGACT150.00462794558.1722158
AACAGGT150.00463185758.159855
ATATTAG453.092282E-1158.11021451
TAACCTA150.004647530658.11020748
TATCTCA201.9883145E-458.11020748
ATACGTT150.004655382658.08544544
AGGTTAG201.99251E-458.0854445
AACTTAT150.00465931258.0730741
ATAATAC303.738205E-758.02362437
CGAGACT150.00467505558.02362436
GTCTAAC258.649178E-657.99893634
ACTAGCC150.00468294157.9989333
ACTGCAT150.004702757.93729825
TGCCGTA150.004702757.93729825
GATACAT202.0199515E-457.92498822
AGGTATA258.736837E-657.90038318
CGTATAA303.7935752E-757.90038314
ACCTATT150.00471458557.90038317
CCTCATA150.00471458557.90038313
Produced by FastQC (version 0.11.5)