FastQCFastQC Report
Fri 17 Jun 2016
SRR1526916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236535
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG10580.44729109856892213No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT10510.4443317056672374No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9970.42150210328281223No Hit
TGAAGCGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCA9930.4198110216247067No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC9360.39571310799670245No Hit
TGAAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT6920.29255712685226287No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC6860.2900205043651045No Hit
TGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.25281670788678207No Hit
TGAAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC5790.24478407001078065No Hit
TGAAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG5780.24436129959625424No Hit
TGAAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA5610.2371742025493056No Hit
TGAAGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA5050.2134990593358277No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA5000.2113852072631957No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4660.1970110131692984No Hit
TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT4420.18686452322066502No Hit
TGAAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.18517344156255947No Hit
TGAAGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC4090.1729130995412941No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3900.16488046166529266No Hit
TGAAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC3830.16192106876360793No Hit
TGAAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT3600.15219734922950093No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA3380.1428964001099203No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG3360.14205085928086753No Hit
TGAAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3190.13486376223391885No Hit
TGAAGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG3130.13232713974676052No Hit
TGAAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA3070.12979051725960217No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2770.11710740482381043No Hit
TGAAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG2760.11668463440928405No Hit
TGAAGCGGGAGCTCGTATAAGTGCACCTGACACACCAGCGTCATCACAGAACTCCACAGAACAG2740.11583909358023126No Hit
TGAAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2740.11583909358023126No Hit
TGAAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2680.1133024710930729No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2570.1086519965332826No Hit
TGAAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC2530.10696091487517702No Hit
TGAAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC2440.10315598114443952No Hit
TGAAGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2370.10019658824275478No Hit
TGAAGCGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC2370.10019658824275478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATT150.00452870358.49153545
AGGCGTA150.004540242758.4539643
GTCGTGT150.004540242758.4539642
TTGATGC150.00454409458.4414539
ATAAGCT150.00454409458.4414539
AACTAGC201.9332119E-458.4414539
TTATGTC150.00457886758.32905625
ATACTAT150.00458274358.31659724
GTACTAT150.00458274358.31659723
AATACTA150.00458274358.31659723
TATCCTA303.6102938E-758.31659724
GCAATAT150.004668631658.043852
CGTATTC150.004680435658.00679846
TATTCAA150.004680435658.00679848
GTTATTA150.004680435658.00679848
ATACCGT202.0143544E-457.95753537
CGGTATT258.7187655E-657.92064736
CAAACAT150.004708065757.92064336
AATACCG202.0207066E-457.92064336
GGGTACG150.004723908457.8715258
Produced by FastQC (version 0.11.5)