FastQCFastQC Report
Fri 17 Jun 2016
SRR1526915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213933
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC21971.0269570379511344No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG12570.5875671355050414No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC11540.5394212206625438No Hit
TGAAGCGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAG9110.4258342565195645No Hit
TGAAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC9080.42443194832026854No Hit
TGAAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG7380.34496781702682616No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC6020.2813965119920723No Hit
TGAAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT5730.2678408660655439No Hit
TGAAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT5730.2678408660655439No Hit
TGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5300.2477411152089673No Hit
TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4910.22951110861811874No Hit
TGAAGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4550.21268341022656628No Hit
TGAAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT4460.20847648562867813No Hit
TGAAGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG4300.20099750856576593No Hit
TGAAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA4270.19959520036646988No Hit
TGAAGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4230.19772545610074183No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC3950.18463724624064543No Hit
TGAAGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC3850.17996288557632528No Hit
TGAAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3580.16734211178266092No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG3560.1664072396497969No Hit
TGAAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA3420.1598631347197487No Hit
TGAAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG3080.14397030846106024No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2920.13649133139814804No Hit
TGAAGCGGGGGTCAAGAGCCAAGAAGCCAGCCAGACCCTCTTGGACTCTGTGTACA2810.13134953466739588No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2700.12620773793664372No Hit
TGAAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2690.12574030187021168No Hit
TGAAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT2600.12153337727232358No Hit
TGAAGCGGGAGCTCGTATAAGTGCACCTGACACACCAGCGTCATCACAGAACTCCA2560.11966363300659554No Hit
TGAAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT2540.11872876087373149No Hit
TGAAGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA2390.11171721987725128No Hit
TGAAGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC2380.11124978381081926No Hit
TGAAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG2320.10844516741222718No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC2260.10564055101363512No Hit
TGAAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2200.10283593461504303No Hit
TGAAGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2170.10143362641574698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGC150.00797604350.69219650
ACCCTAT150.00798354950.68014549
AAACGAT150.00798354950.68014549
CTAATCG150.00798354950.68014549
CTCTTAT150.00799105850.66809548
CTTGTAC251.9244237E-550.6680948
CTAACCT150.00823416450.2855147
CTACCCT150.00823416450.2855147
CGATTCT150.00825723450.24994343
ACGATTC150.00826493550.23809442
GTTTAGG150.0082726450.22625441
GACGATT150.0082726450.22625441
CACGTCT204.112715E-450.15532740
CTCAATT355.1733878E-850.10816239
TCACGTC204.131861E-450.10816239
CGTGATG150.00834999450.1081639
TCTCAAT355.1828465E-850.09638238
ATCAAAT204.1366587E-450.0963838
CGTCACG204.1414605E-450.08460637
GTACATT204.146267E-450.07283836
Produced by FastQC (version 0.11.5)