FastQCFastQC Report
Fri 17 Jun 2016
SRR1526914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154119
Sequences flagged as poor quality0
Sequence length64
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT95786.21467826809154No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.3211803865843926No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT4050.2627839526599575No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3900.25305121367255173No Hit
AAAGTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT3170.20568521726717667No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT2750.17843354810244033No Hit
AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.13431179802620052No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT2010.1304187024312382No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.12328136050714059No Hit
AAAGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1570.10186933473484774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT150.00450599358.55599656
CCATGAA258.159042E-658.55599257
ATTAGGC150.004654316758.0791551
AAGACTA351.6112608E-858.0791548
TCCTAAA150.004654316758.0791550
CATAGAC150.004654316758.0791547
AGATTGG150.004654316758.0791549
ATTGGTC150.004654316758.0791551
TTACGCC650.058.0791552
GATTAAC150.004654316758.0791550
TATGTTA150.004666337358.0413446
GGTAGAC150.004666337358.0413445
GCATAGA150.004666337358.0413446
ATAGCAT150.004672356458.02245343
TGACTGA150.004672356458.02245344
CAGCGTA258.63091E-658.0035836
CCGATAG150.004678381658.0035840
CGTCTCA303.7385507E-758.0035841
TATGAAA150.004678381658.0035838
GCCGATA150.004678381658.0035839
Produced by FastQC (version 0.11.5)