FastQCFastQC Report
Fri 17 Jun 2016
SRR1526913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214696
Sequences flagged as poor quality0
Sequence length64
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2277410.60755673137832No Hit
AAAGTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG9080.42292357566046873No Hit
AAAGTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT8830.411279204083914No Hit
AAAGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG7540.35119424674889144No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.3479338227074561No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5820.27108097030219475No Hit
AAAGTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC5790.2696836457130082No Hit
AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4080.19003614412937364No Hit
AAAGTTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG3000.13973245891865707No Hit
AAAGTTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG2990.1392666840555949No Hit
AAAGTTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG2930.13647203487722176No Hit
AAAGTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT2700.12575921302679138No Hit
AAAGTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC2650.12343033871148042No Hit
AAAGTTGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT2530.11784104035473414No Hit
AAAGTTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT2470.115046391176361No Hit
AAAGTTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCGAAGGTCC2450.1141148414502366No Hit
AAAGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA2400.11178596713492567No Hit
AAAGTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2380.11085441740880128No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.10945709281961472No Hit
AAAGTTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGT2190.10200469501061968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT501.8189894E-1258.2333558
TACCATT201.9694927E-458.219757
GTAGAAT201.9694927E-458.219757
CTACACT150.00461245358.21969656
TACACTA150.00461245358.21969657
AACCTAC150.00461673758.2060553
CTATGAA150.004638203458.1379148
TGATACA150.004638203458.1379150
ACTGAAT150.004638203458.1379146
CAGATTA150.004638203458.1379148
GAACCCC150.004638203458.1379150
TAAATAC150.004638203458.1379152
CAATCCC150.004638203458.1379148
GTAACTA201.9832322E-458.1379148
ATATTAG150.004638203458.1379151
TCGGACG150.00464250558.12430240
CTCATAT150.00464250558.12430245
CAACTTA150.00464250558.12430244
CGATCAA150.00464250558.12430240
GCCGATA150.004651118558.097139
Produced by FastQC (version 0.11.5)