FastQCFastQC Report
Fri 17 Jun 2016
SRR1526912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212872
Sequences flagged as poor quality0
Sequence length64
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT209429.837836822127851No Hit
AAAGTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT8640.40587771054906235No Hit
AAAGTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG8240.3870870758014206No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.3368221278514788No Hit
AAAGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG6630.31145477094216245No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5080.23864106129505053No Hit
AAAGTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC4690.22032019241609982No Hit
AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3580.1681761809913939No Hit
AAAGTTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG3110.1460971851629148No Hit
AAAGTTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG2900.13623210192040286No Hit
AAAGTTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG2760.12965537975872823No Hit
AAAGTTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT2540.11932053064752526No Hit
AAAGTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT2470.11603216956668795No Hit
AAAGTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC2420.11368334022323273No Hit
AAAGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA2400.11274380848585064No Hit
AAAGTTGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT2330.10945544740501333No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.10710661806155812No Hit
AAAGTTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCGAAGGTCC2260.10616708632417603No Hit
AAAGTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2250.105697320455485No Hit
AAAGTTGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA2190.10287872524333871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGGC150.004559204458.39045726
ATAAGAT150.004559204458.39045726
GCCTTCA258.319819E-658.376641
CACATGA150.00456349258.376642
CAACTAG150.00456349258.376642
AATATAA150.00468038958.0049557
CACTCTC258.640964E-658.0049556
CTAGTAC150.00468038958.0049558
ATATAAG150.00468038958.0049558
CGCTAGT150.00468038958.0049556
ACCAGTA202.0057708E-458.0049557
ACTACAC351.6316335E-858.0049556
TACTACA351.6316335E-858.0049555
GTCACAA258.665156E-657.9776153
ACAAATG150.00468913657.97760853
CTTACAA150.00468913657.97760850
AGGTCGA150.00468913657.97760852
CGGTTAC150.00471105657.90936737
TAGACTG202.0269072E-457.88212236
AAGTTAG258.762485E-657.8685042
Produced by FastQC (version 0.11.5)