FastQCFastQC Report
Fri 17 Jun 2016
SRR1526911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191644
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2385912.44964621903112No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8250.4304856922209931No Hit
AAAGTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC7570.3950032351652022No Hit
AAAGTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC7560.3944814343261464No Hit
AAAGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6240.3256037235707875No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5540.2890776648368851No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA5190.27081463546993384Illumina Single End Adapter 1 (95% over 21bp)
AAAGTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG4230.22072175492058194No Hit
AAAGTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2860.14923503996994428No Hit
AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2820.14714783661372127No Hit
AAAGTTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC2800.14610423493560978No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2710.14140802738410804No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2430.12679760389054706No Hit
AAAGTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC2390.12471040053432406No Hit
AAAGTTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT2260.11792698962659932No Hit
AAAGTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG2220.11583978627037633No Hit
AAAGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA2210.11531798543132057No Hit
AAAGTTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC2200.11479618459226482No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG2170.11323078207509758No Hit
AAAGTTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC2170.11323078207509758No Hit
AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.10644737116737285No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2040.10644737116737285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATAAG150.00794098550.7466149
CACGAGC150.00794098550.7466149
ATAAGTT150.00794098550.7466150
GATTCCA150.00794098550.7466150
GTGACCT150.00794098550.7466150
AGATTAG251.906091E-550.74660549
ATGACGA203.8804745E-450.74660549
AGGATGT203.8804745E-450.74660550
CCCCGCT150.00820325150.3313146
CGTGTTG150.00820325150.3313145
TCAAAGT150.00820325150.3313145
CGGATCA204.0411338E-450.33130647
TGTCATT150.0082203950.30475244
CGTACTG301.0113799E-650.2252442
TAGTCGG204.08344E-450.22523543
CTCAATT150.00829784550.18557741
ACGTACT301.0169097E-650.18557741
TATATAA150.008332442550.13279338
ATATAAT150.008332442550.13279339
TAAATCT150.008332442550.13279338
Produced by FastQC (version 0.11.5)