##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526911_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 191644 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.44181398843689 39.0 38.0 40.0 35.0 40.0 2 38.34263530295757 39.0 38.0 40.0 35.0 40.0 3 38.201482957984595 39.0 38.0 40.0 35.0 40.0 4 38.19356202124773 39.0 38.0 40.0 35.0 40.0 5 37.99169293064223 39.0 38.0 40.0 35.0 40.0 6 38.56372231846549 39.0 38.0 40.0 36.0 40.0 7 38.494964621903115 39.0 38.0 40.0 36.0 40.0 8 38.51783515267893 39.0 38.0 40.0 36.0 40.0 9 38.53885850848448 39.0 38.0 40.0 36.0 40.0 10 37.99396798230052 39.0 38.0 40.0 35.0 40.0 11 38.02817724530901 39.0 38.0 40.0 35.0 40.0 12 37.969730333326375 39.0 38.0 40.0 35.0 40.0 13 37.77218697167665 39.0 38.0 40.0 34.0 40.0 14 37.98266577612657 39.0 38.0 40.0 35.0 40.0 15 37.90846569681284 39.0 38.0 40.0 35.0 40.0 16 37.8066414810795 39.0 38.0 40.0 34.0 40.0 17 37.72970194736073 39.0 38.0 40.0 33.0 40.0 18 37.69939053661998 39.0 38.0 40.0 33.0 40.0 19 37.57126755859824 39.0 38.0 40.0 33.0 40.0 20 37.5019358811129 39.0 38.0 40.0 33.0 40.0 21 37.49587255536307 39.0 38.0 40.0 33.0 40.0 22 37.40585669261756 39.0 38.0 40.0 33.0 40.0 23 37.33969234622529 39.0 37.0 40.0 33.0 40.0 24 37.12985535680741 39.0 37.0 40.0 33.0 40.0 25 36.94828431884118 39.0 36.0 40.0 33.0 40.0 26 36.64046356786542 39.0 36.0 40.0 32.0 40.0 27 36.59501471478366 39.0 36.0 40.0 31.0 40.0 28 36.12500782701259 38.0 35.0 40.0 30.0 40.0 29 36.16567698440859 38.0 35.0 40.0 30.0 40.0 30 35.79677422721296 38.0 35.0 40.0 29.0 40.0 31 35.294394815386866 38.0 35.0 40.0 28.0 40.0 32 35.12465300244203 38.0 35.0 40.0 28.0 40.0 33 34.952881384233265 38.0 34.0 40.0 27.0 40.0 34 35.16591179478617 38.0 35.0 40.0 28.0 40.0 35 34.9158387426687 38.0 34.0 40.0 27.0 40.0 36 34.827529168666906 38.0 35.0 40.0 27.0 40.0 37 34.52199912337459 38.0 34.0 40.0 25.0 40.0 38 34.523272317421885 38.0 34.0 40.0 25.0 40.0 39 34.36788524555948 38.0 34.0 40.0 24.0 40.0 40 34.03466322973847 38.0 33.0 40.0 23.0 40.0 41 33.72842353530505 38.0 33.0 40.0 21.0 40.0 42 33.49785539855148 38.0 33.0 39.0 19.0 40.0 43 33.27416981486506 38.0 33.0 39.0 18.0 40.0 44 33.19397424391058 38.0 33.0 39.0 17.0 40.0 45 32.82047442132287 37.0 32.0 39.0 11.0 40.0 46 32.388125900106445 37.0 32.0 39.0 4.0 40.0 47 32.003621297823045 36.0 31.0 39.0 4.0 40.0 48 31.821554549059716 36.0 31.0 39.0 4.0 40.0 49 31.50081922731732 36.0 31.0 39.0 4.0 40.0 50 31.227572999937383 36.0 31.0 39.0 4.0 40.0 51 30.260629083091565 36.0 29.0 39.0 4.0 40.0 52 30.0930057815533 36.0 29.0 39.0 4.0 40.0 53 29.81383189664169 35.0 29.0 39.0 4.0 40.0 54 29.352685187117782 35.0 28.0 39.0 4.0 40.0 55 28.984872993675772 35.0 27.0 39.0 4.0 40.0 56 17.525938719709462 21.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.9484352885156895 1 2 -1.2850718458461685 1 3 -0.9821196663918386 1 4 -1.6284328090464655 1 5 -1.7602178442682188 1 6 -1.398848196420552 1 7 -0.8765437265029448 1 8 -1.4696782844796985 1 9 -1.3001250635787756 1 10 -0.8413595257214084 1 11 -0.7987790417196976 1 12 -1.1969190287070361 1 13 -0.5613379077809455 1 14 -0.9918025573470786 1 15 -0.9574720664810741 1 16 -0.7776746072424032 1 17 -0.4969006651868071 1 18 -1.1241418375057322 1 19 -1.165342544644787 1 20 -1.1421054102944908 1 21 -0.3394487891919056 1 22 -0.2729642401042369 1 23 -0.6752648268284602 1 24 -0.7425237362751389 1 25 -0.7384014224617488 1 26 -1.166138927846447 1 27 -1.6554953183790317 1 28 -1.0495312930005625 1 29 -1.2082072669293638 1 30 -1.3853738248516763 1 31 -1.1015422429318278 1 32 -1.1609133073101603 1 33 -1.243811427507218 1 34 -1.5416642905833484 1 35 -1.622965443803075 1 36 -0.7952314091994381 1 37 -0.8305232496559682 1 38 -0.7078902966242993 1 39 -0.2674860247344881 1 40 -0.5907895084405581 1 41 -0.40233082883162297 1 42 -0.3397909896488187 1 43 -0.4729631270631529 1 44 -0.15666153333323507 1 45 -0.9771501168229797 1 46 -0.5436947080028816 1 47 -0.17566108825939963 1 48 -0.6144704305551585 1 49 -0.4635248126579228 1 50 -0.4495593477348443 1 51 0.5491451233570501 1 52 0.5248582311415362 1 53 0.6450002885254484 1 54 0.23884464774457825 1 55 0.11152433694019592 1 56 0.6577357018378471 2 1 -0.16145585733234924 2 2 -0.0508793623520134 2 3 -0.239388584624443 2 4 -0.39116135145378195 2 5 0.056567325868911666 2 6 -0.1177727774261399 2 7 0.04311726892620982 2 8 0.14481511318408735 2 9 0.07896965455225313 2 10 -0.11170614837250525 2 11 -0.7606886405008026 2 12 -0.2348189779198293 2 13 -0.16557863911664583 2 14 -0.09417051062285253 2 15 -0.05492000959940668 2 16 -0.15749939139679725 2 17 -0.3393968561466849 2 18 0.022601433190054365 2 19 -0.21463787222224084 2 20 0.2735560930066825 2 21 -0.24549246618530418 2 22 -0.10590608875837404 2 23 0.03531160790998911 2 24 -0.17326269005878459 2 25 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2.0242480172070962 3 51 1.9311393320206562 3 52 1.4405913616805996 3 53 1.620460231782051 3 54 1.6369551511228586 3 55 1.5328586842448857 3 56 0.31461364503595135 4 1 -0.41168056200620384 4 2 -0.5384885046221726 4 3 -0.4991792882710797 4 4 -0.7856464567439687 4 5 -0.7969252267761888 4 6 -0.22758916160692877 4 7 -0.3384633679510358 4 8 -0.09622880052931748 4 9 -0.11661971370660495 4 10 -0.7889339347104567 4 11 -0.3328509414766643 4 12 -0.10904856189517886 4 13 -0.0447517259168535 4 14 0.1041795150576803 4 15 -0.6021079911094205 4 16 0.06470143038029619 4 17 -0.2455987051451416 4 18 -0.3884476166301809 4 19 -0.09820875049651079 4 20 -0.2620435474658436 4 21 -0.5973157841103145 4 22 -0.034161096462540286 4 23 -0.2720201845912982 4 24 -0.3086633063916082 4 25 -0.20499233872598666 4 26 -0.22882238585231818 4 27 -0.2665485645994039 4 28 -0.5007511901062642 4 29 -0.09584417000041157 4 30 0.17805135584460174 4 31 -0.12840207312467555 4 32 -0.3207466564248307 4 33 -0.3557010844210211 4 34 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-0.0317973378997749 120 52 -0.018366842241665182 120 53 0.08915014807157462 120 54 -0.005071648060742007 120 55 0.0253898168505593 120 56 0.2514610435037241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 157.0 5 20.0 6 29.0 7 44.0 8 82.0 9 134.0 10 111.0 11 157.0 12 185.0 13 219.0 14 395.0 15 462.0 16 595.0 17 803.0 18 949.0 19 1002.0 20 1159.0 21 1358.0 22 1494.0 23 1476.0 24 1941.0 25 2239.0 26 2335.0 27 2694.0 28 3253.0 29 3538.0 30 4155.0 31 4945.0 32 6184.0 33 7509.0 34 10046.0 35 13452.0 36 18721.0 37 27664.0 38 35759.0 39 36378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.75527540648285 0.16175826010728225 0.05583268977896517 0.02713364363089896 8 99.52447070614274 0.3439888086190337 0.0642042844615192 0.06733620077671525 9 98.46903633821043 0.7179979545407109 0.2791634488948258 0.5338022583540315 10 45.91221222683726 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25.181064891152342 21.73822295506251 18.12579574627956 34.95491640750558 24 24.066063088684217 21.4663292196102 33.86229029143946 20.60531740026613 25 40.790613680727354 17.960521508575248 19.895823547218654 21.35304126347874 26 24.249986947214534 17.68078107868219 21.75794914634783 36.31128282775544 27 23.063226763159406 16.389667757953603 21.677448999305547 38.86965647958145 28 25.388094939618068 33.318185616982156 20.894968868831224 20.39875057456855 29 41.31630114360979 18.48016976437875 20.367544061382787 19.835985030628674 30 25.29202215863617 18.545564872598305 36.285551376545115 19.876861592220415 31 25.74003516998828 33.32827415843242 22.400456372466923 18.531234299112377 32 39.78500813808046 19.56227070762049 22.79761562092769 17.85510553337136 33 24.751101851948786 19.80663937772846 22.547397954376525 32.89486081594623 34 22.11948753658396 35.78762192472212 24.523165042748914 17.569725495945004 35 21.545262650652894 23.561300865812203 39.37219449085738 15.521241992677522 36 23.09868896783727 37.04949644208298 22.407541316900893 17.444273273178858 37 21.924878692922793 23.302486926083905 37.77182741382744 17.000806967165868 38 37.79574204845111 22.58727437627458 22.730394392631155 16.886589182643156 39 22.81682752832564 21.48709609735627 22.263428451531684 33.4326479227864 40 21.44379468550851 21.735661266784952 38.06076704217367 18.759777005532865 41 21.362638747414074 21.488653428124376 37.63270921062304 19.515998613838512 42 33.77922030177462 21.148907340591467 24.29952680311123 20.77234555452268 43 19.669503993274486 19.97740647330811 39.58858764186633 20.76450189155107 44 20.53388953393682 20.16244700245352 25.469294259190185 33.83436920441948 45 20.237238073715137 20.677657891555818 39.307731522601735 19.77737251212731 46 19.33782703794061 21.14021620257424 25.146168082779507 34.375788676705646 47 19.74266987890966 35.47021135576994 26.07808275383207 18.70903601148833 48 20.82006641790652 34.79572020272724 24.61567609955318 19.76853727981306 49 20.885180177099173 35.251995391001934 23.83630690876183 20.026517523137066 50 33.95213174825469 21.94452926744517 24.350051069296295 19.753287915003842 51 19.68219212691261 35.593113285506 25.8572864744801 18.867408113101288 52 20.915968642894374 22.19162792656667 38.069716385614484 18.82268704492448 53 20.500292214459087 21.36008719047644 38.39487387392131 19.744746721143162 54 21.75692244001083 35.14057184104794 23.791326316683385 19.311179402257853 55 19.957332490518333 35.709544879898864 23.18636746734092 21.146755162241888 56 33.627908446476155 22.440198443210903 22.713531846764763 21.218361263548175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.5 2 3.0 3 6.0 4 11.5 5 14.0 6 101.0 7 188.0 8 193.5 9 199.0 10 211.5 11 224.0 12 235.0 13 410.5 14 575.0 15 669.0 16 763.0 17 848.0 18 933.0 19 1275.0 20 1617.0 21 1943.5 22 2566.0 23 2862.0 24 3303.5 25 3745.0 26 4077.0 27 4409.0 28 4406.0 29 3733.5 30 3064.0 31 3115.0 32 3166.0 33 2893.5 34 2621.0 35 2532.0 36 2443.0 37 2409.5 38 2672.0 39 2968.0 40 3882.0 41 4796.0 42 17234.5 43 29673.0 44 18055.0 45 6437.0 46 6135.5 47 6801.5 48 7769.0 49 7844.5 50 7920.0 51 8273.0 52 8626.0 53 8974.0 54 10484.5 55 11647.0 56 12198.0 57 12749.0 58 11992.0 59 11235.0 60 10180.0 61 9125.0 62 8067.0 63 6456.0 64 5903.0 65 4896.0 66 3889.0 67 3617.5 68 3346.0 69 3050.0 70 2754.0 71 2335.0 72 1508.0 73 1100.0 74 872.5 75 645.0 76 551.0 77 457.0 78 339.5 79 162.0 80 102.0 81 75.0 82 48.0 83 31.5 84 15.0 85 9.0 86 3.0 87 2.5 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03548245705579094 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0015654025171672474 21 0.0 22 0.0 23 0.0 24 0.004696207551501743 25 0.024002838596564463 26 0.059485295652355406 27 0.06626870656008015 28 0.10227296445492684 29 0.16697626849783975 30 0.24889900022959238 31 0.29847007993988856 32 0.2958610757446098 33 0.314124105111561 34 0.3365615411909582 35 0.3783056083154182 36 0.41848427292271084 37 0.4205714762789339 38 0.46701175095489555 39 0.5238880424119722 40 0.5979837615578886 41 0.6209429984763416 42 0.6454676379119618 43 0.6908643109098119 44 0.6809500949677527 45 0.7153889503454322 46 0.7576548183089479 47 0.8025296904677423 48 0.85314437185615 49 0.8249671265471394 50 0.8901922314291081 51 0.8635803886372649 52 0.8886268289119408 53 0.89488843898061 54 1.6880257143453488 55 0.9413287136565717 56 0.921500281772453 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 191644.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.943728997516224 #Duplication Level Percentage of deduplicated Percentage of total 1 69.9335114104872 25.13671181983261 2 12.58492538180129 9.04698294754858 3 5.853318622611927 6.3117029492183425 4 3.1995819058126704 4.600196197115485 5 2.0077231287381685 3.608252802070506 6 1.3384820858254458 2.8866022416564046 7 0.8971604436443877 2.257310429755171 8 0.7113408048313106 2.045459289098537 9 0.5226177341617793 1.6906347185406276 >10 2.6174438185935776 16.807205025985684 >50 0.22356425294698334 5.4789088100853665 >100 0.10016839904767436 5.574920164471624 >500 0.008710295569362987 2.1054663855899483 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0014517159282271645 12.44964621903112 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 23859 12.44964621903112 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 825 0.4304856922209931 No Hit AAAGTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 757 0.3950032351652022 No Hit AAAGTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 756 0.3944814343261464 No Hit AAAGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 624 0.3256037235707875 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 554 0.2890776648368851 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA 519 0.27081463546993384 Illumina Single End Adapter 1 (95% over 21bp) AAAGTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 423 0.22072175492058194 No Hit AAAGTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 286 0.14923503996994428 No Hit AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 282 0.14714783661372127 No Hit AAAGTTGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 280 0.14610423493560978 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 271 0.14140802738410804 No Hit AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 243 0.12679760389054706 No Hit AAAGTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 239 0.12471040053432406 No Hit AAAGTTGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 226 0.11792698962659932 No Hit AAAGTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 222 0.11583978627037633 No Hit AAAGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 221 0.11531798543132057 No Hit AAAGTTGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC 220 0.11479618459226482 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG 217 0.11323078207509758 No Hit AAAGTTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 217 0.11323078207509758 No Hit AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 204 0.10644737116737285 No Hit AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 204 0.10644737116737285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.218008390557492E-4 0.0 0.0 0.0 0.0 8 5.218008390557492E-4 0.0 0.0 0.0 0.0 9 0.0010436016781114985 0.0 0.0 0.0 0.0 10 0.010957817620170732 0.0 0.0 0.0 0.0 11 0.022437436079397216 0.0 0.0 0.0 0.0 12 0.022959236918452964 0.0 0.0 0.0 0.0 13 0.025046440274675962 0.0 0.0 0.0 0.0 14 0.028177245309010456 0.0 0.0 0.0 0.0 15 0.0318298511824007 0.0 0.0 0.0 0.0 16 0.036004257894846695 0.0 0.0 0.0 0.0 17 0.03756966041201394 0.0 0.0 0.0 0.0 18 0.040700465446348436 0.0 0.0 0.0 0.0 19 0.04644027467596168 0.0 0.0 0.0 0.0 20 0.05583268977896517 0.0 0.0 0.0 0.0 21 0.06105069816952265 0.0 0.0 0.0 0.0 22 0.0667905073991359 0.0 0.0 0.0 0.0 23 0.07461751998497214 0.0 0.0 0.0 0.0 24 0.07983552837552962 0.0 0.0 0.0 0.0 25 0.08661893928325437 0.0 0.0 0.0 0.0 26 0.09288054935192336 0.0 0.0 0.0 0.0 27 0.09548955354720211 0.0 0.0 0.0 0.0 28 0.10905637536265159 0.0 0.0 0.0 0.0 29 0.11688338794848782 0.0 0.0 0.0 0.0 30 0.12366679885621257 0.0 0.0 0.0 0.0 31 0.12888480724677004 0.0 0.0 0.0 0.0 32 0.13097201060299304 0.0 0.0 0.0 0.0 33 0.13201561228110456 0.0 0.0 0.0 0.0 34 0.13879902318882928 0.0 0.0 0.0 0.0 35 0.14819143829183276 0.0 0.0 0.0 0.0 36 0.15549665003861327 0.0 0.0 0.0 0.0 37 0.160192857590115 0.0 0.0 0.0 0.0 38 0.164367264302561 0.0 0.0 0.0 0.0 39 0.1695852726931185 0.0 0.0 0.0 0.0 40 0.17584688276178748 0.0 0.0 0.0 0.0 41 0.18471749702573523 0.0 0.0 0.0 0.0 42 0.19254450961157146 0.0 0.0 0.0 0.0 43 0.1972407171630732 0.0 0.0 0.0 0.0 44 0.20976393730041118 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAATAAG 15 0.007940985 50.74661 49 CACGAGC 15 0.007940985 50.74661 49 ATAAGTT 15 0.007940985 50.74661 50 GATTCCA 15 0.007940985 50.74661 50 GTGACCT 15 0.007940985 50.74661 50 AGATTAG 25 1.906091E-5 50.746605 49 ATGACGA 20 3.8804745E-4 50.746605 49 AGGATGT 20 3.8804745E-4 50.746605 50 CCCCGCT 15 0.008203251 50.33131 46 CGTGTTG 15 0.008203251 50.33131 45 TCAAAGT 15 0.008203251 50.33131 45 CGGATCA 20 4.0411338E-4 50.331306 47 TGTCATT 15 0.00822039 50.304752 44 CGTACTG 30 1.0113799E-6 50.22524 42 TAGTCGG 20 4.08344E-4 50.225235 43 CTCAATT 15 0.008297845 50.185577 41 ACGTACT 30 1.0169097E-6 50.185577 41 TATATAA 15 0.0083324425 50.132793 38 ATATAAT 15 0.0083324425 50.132793 39 TAAATCT 15 0.0083324425 50.132793 38 >>END_MODULE