##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526907_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 344049 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.52296329883244 39.0 38.0 40.0 36.0 40.0 2 38.446302706881866 39.0 38.0 40.0 35.0 40.0 3 38.156817778862894 39.0 38.0 40.0 35.0 40.0 4 37.98347328432869 39.0 38.0 40.0 35.0 40.0 5 38.29657984763798 39.0 38.0 40.0 35.0 40.0 6 38.42259968783517 39.0 38.0 40.0 36.0 40.0 7 38.26901400672578 39.0 38.0 40.0 35.0 40.0 8 38.30438978168807 39.0 38.0 40.0 35.0 40.0 9 38.28702016282564 39.0 38.0 40.0 35.0 40.0 10 37.477388395257655 39.0 38.0 40.0 33.0 40.0 11 37.18203511709088 39.0 37.0 40.0 33.0 40.0 12 37.030187560492834 39.0 36.0 40.0 32.0 40.0 13 36.754282093539004 39.0 36.0 40.0 31.0 40.0 14 37.25030736900848 39.0 37.0 40.0 33.0 40.0 15 37.13247822257876 39.0 36.0 40.0 32.0 40.0 16 37.001624768564945 39.0 36.0 40.0 32.0 40.0 17 36.89143116242163 39.0 36.0 40.0 31.0 40.0 18 36.7753052617505 39.0 36.0 40.0 31.0 40.0 19 36.803902932431136 39.0 36.0 40.0 31.0 40.0 20 36.748320733383906 39.0 36.0 40.0 31.0 40.0 21 36.904452563442995 39.0 36.0 40.0 32.0 40.0 22 36.79956052771553 39.0 36.0 40.0 32.0 40.0 23 36.71483131763208 39.0 36.0 40.0 31.0 40.0 24 36.575310493563414 39.0 36.0 40.0 31.0 40.0 25 36.44003906420306 39.0 36.0 40.0 31.0 40.0 26 36.254460847146774 39.0 36.0 40.0 30.0 40.0 27 36.14847013070813 39.0 36.0 40.0 30.0 40.0 28 35.77803743071481 38.0 35.0 40.0 29.0 40.0 29 35.798107246351535 39.0 35.0 40.0 29.0 40.0 30 35.35161851945508 38.0 35.0 40.0 29.0 40.0 31 34.77129420518589 38.0 34.0 40.0 27.0 40.0 32 34.46685791849417 38.0 34.0 40.0 25.0 40.0 33 34.335292356611994 38.0 33.0 40.0 25.0 40.0 34 34.419300739138905 38.0 34.0 40.0 25.0 40.0 35 34.193152719525415 38.0 33.0 40.0 24.0 40.0 36 34.023836720932195 38.0 34.0 40.0 22.0 40.0 37 33.79139889957535 38.0 33.0 40.0 21.0 40.0 38 33.74937000252871 38.0 33.0 40.0 20.0 40.0 39 33.54237041816718 38.0 33.0 40.0 18.0 40.0 40 33.23935253408671 38.0 33.0 40.0 16.0 40.0 41 33.026475879889205 38.0 33.0 40.0 4.0 40.0 42 32.85816264543713 38.0 33.0 39.0 4.0 40.0 43 32.622077087856674 37.0 32.0 39.0 4.0 40.0 44 32.56214085784293 37.0 32.0 39.0 4.0 40.0 45 32.251016570314114 37.0 32.0 39.0 4.0 40.0 46 31.89362271071853 37.0 32.0 39.0 4.0 40.0 47 31.582867556656176 36.0 31.0 39.0 4.0 40.0 48 31.30667433999227 36.0 31.0 39.0 4.0 40.0 49 31.065665065150604 36.0 31.0 39.0 4.0 40.0 50 30.78562065287212 36.0 31.0 39.0 4.0 40.0 51 29.872305979671502 35.0 29.0 39.0 4.0 40.0 52 29.626021293478544 35.0 29.0 39.0 4.0 40.0 53 29.29637929480975 35.0 28.0 39.0 4.0 40.0 54 28.75884249045921 35.0 26.0 39.0 4.0 40.0 55 28.369703152748592 35.0 23.0 39.0 4.0 40.0 56 16.62674793416054 20.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.7142744994176908 1 2 -1.024162060209271 1 3 -0.734375001491216 1 4 -0.9588155704121633 1 5 -1.1109262251058283 1 6 -1.023054708665292 1 7 -0.6033517381018427 1 8 -0.6527339033332282 1 9 -0.6662555979247813 1 10 -0.8206982955923223 1 11 -0.8879495179487336 1 12 -1.4202273112168982 1 13 -1.1162803182223513 1 14 -1.3370133252042251 1 15 -1.4539510613605415 1 16 -0.5697688504080176 1 17 -0.9047067563399267 1 18 -1.0632037931035896 1 19 -1.1242227934655062 1 20 -1.139433080546219 1 21 -0.8854948426724008 1 22 -1.4983151707300948 1 23 -0.9496268906183474 1 24 -1.1314827483787795 1 25 -1.4114098824430599 1 26 -1.1130584472171208 1 27 -1.6961149405416123 1 28 -0.9082439310449928 1 29 -0.928639455604511 1 30 -0.984627222534165 1 31 -1.060944916245525 1 32 -1.0305282608267063 1 33 -1.1371256890264974 1 34 -1.9095209310821488 1 35 -2.162369463156516 1 36 -0.6330019196001544 1 37 -0.30844163677100767 1 38 -0.2083425204279692 1 39 -0.7214390965383402 1 40 -0.7357506118947015 1 41 -0.5540814868228878 1 42 -0.7024794399437582 1 43 -0.5810313504445759 1 44 -0.8751560488570043 1 45 -0.38808969487012845 1 46 -0.8059728998241269 1 47 0.14289476884120944 1 48 -0.3551298408260024 1 49 -0.42855973462678776 1 50 -0.6873096888983561 1 51 -0.06779740502045684 1 52 -0.21691344706298565 1 53 -0.28162136148701933 1 54 -0.27397827485172144 1 55 -0.5271130097363574 1 56 -0.08077552707332103 2 1 -0.3344792762535178 2 2 -0.17803501340866035 2 3 -0.31659541723851703 2 4 -0.26768407194121835 2 5 -0.3275774077017317 2 6 -0.026867661090484773 2 7 -0.07492879070897374 2 8 -0.02591936379078419 2 9 -0.20963035114740336 2 10 -0.39941544032967613 2 11 -0.5184318467529749 2 12 -1.1659691917572985 2 13 -0.5573827840606214 2 14 -0.4421308105911308 2 15 -0.3363785765542886 2 16 -0.4114248717760418 2 17 -0.8349026298350708 2 18 -0.6543952923487453 2 19 -0.5762028190142203 2 20 -0.6229231900960173 2 21 -0.7195346367342594 2 22 -0.7942157629957975 2 23 -0.5879673853344087 2 24 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0.612870069860044 3 48 0.572278309837781 3 49 0.4282247667625292 3 50 0.4485347075202917 3 51 0.5702467444083794 3 52 0.2642921719552298 3 53 0.2113548657436084 3 54 0.6670529076119998 3 55 0.4713047117826292 3 56 -0.3791700932974642 4 1 -0.04682230442711699 4 2 -0.19164311134235135 4 3 -0.2212550753629401 4 4 -0.5059280324182396 4 5 -0.29688482616003853 4 6 -0.15077199541840258 4 7 -0.2918496796970089 4 8 -0.12176164370490028 4 9 -0.23737637049990923 4 10 -0.16585623487900136 4 11 -0.5710609796085393 4 12 -0.31112223638330505 4 13 -0.16660543076130807 4 14 -0.6204768318026623 4 15 -0.7272860619376686 4 16 -1.0079782305780824 4 17 -1.0725052250406293 4 18 -0.8955207489729347 4 19 -0.7115030058467084 4 20 -0.7302914545987704 4 21 -0.6631967394828351 4 22 -0.5999657420817144 4 23 -0.5007695938617758 4 24 -0.5455010624105583 4 25 -0.6655651669646261 4 26 -0.5596649648578449 4 27 -0.6559952837373331 4 28 -0.9026169832553776 4 29 -0.7587537259288553 4 30 -0.5100206279437316 4 31 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120 56 0.12002456846047593 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 792.0 5 186.0 6 133.0 7 172.0 8 468.0 9 658.0 10 401.0 11 588.0 12 636.0 13 688.0 14 942.0 15 1181.0 16 1433.0 17 1816.0 18 2086.0 19 2475.0 20 2520.0 21 2999.0 22 3311.0 23 3197.0 24 3947.0 25 4541.0 26 4598.0 27 5075.0 28 6134.0 29 6594.0 30 7591.0 31 9434.0 32 11496.0 33 13808.0 34 17998.0 35 23015.0 36 31013.0 37 46810.0 38 59487.0 39 65826.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.55267999616333 0.29734136707271347 0.08225572520193344 0.06772291156201588 8 99.20160028842407 0.5358523920008839 0.15467904098994587 0.10786827858509382 9 97.80757973428203 1.273655787402376 0.3929672808233711 0.5257971974922176 10 48.67388075535752 29.47051146784324 12.435728631677465 9.41987914512177 11 32.61948152734058 41.32870608547039 15.400131957947853 10.651680429241184 12 42.8238419527451 14.491249792907407 20.814186351362743 21.870721902984748 13 26.726716252626808 15.913721591982538 27.17868675682824 30.180875398562414 14 31.160677694165656 20.457551104639165 27.81260808780145 20.56916311339373 15 32.926123895142844 22.563937113608816 19.417292304293866 25.092646686954474 16 37.06100003197219 25.081892404860934 21.577159067458414 16.27994849570846 17 36.399757011355945 17.675098605140548 28.67789181192214 17.247252571581374 18 28.25149905972696 17.974765222395646 35.02001168438217 18.753724033495228 19 39.43159259291554 16.681635464715782 22.54736970605931 21.33940223630936 20 19.688179418911535 29.1663275627536 25.685086791224375 25.46040622711049 21 21.371374426317182 22.08406360721874 40.12131992826603 16.423242038198048 22 22.37442922374429 24.72671043950135 25.09700653104645 27.80185380570791 23 25.60129516435159 19.81810730448279 28.47879226505527 26.10180526611035 24 28.779224403303306 20.259055459146154 29.551274784241567 21.410445353308972 25 29.92575106547512 15.665246033176542 28.007605137537865 26.401397763810476 26 27.34721062872466 14.85357291397027 32.08395017203619 25.71526628526887 27 28.80822053271751 14.786721035884847 27.325185706557463 29.07987272484018 28 38.65639375361884 25.37948773156971 20.31936034822382 15.644758166587621 29 49.93987812582795 17.46096445385173 18.039764869609776 14.559392550710545 30 40.91335563351554 20.717316052634647 23.810883576731666 14.558444737118148 31 38.17006838414048 30.04733838198343 16.738665314141468 15.043927919734626 32 40.19087429639152 26.757381512477664 18.43942481190953 14.612319379221287 33 29.053524099986294 30.48135826478932 17.19582350488531 23.269294130339073 34 22.653092173182696 42.62317073882029 18.88170826309865 15.842028824898366 35 20.678195563310215 39.709628194542105 26.27672269667642 13.335453545471257 36 20.79392877991827 47.116462346760066 17.07880910683012 15.010799766491537 37 19.101730338128153 40.40111853166449 26.229742667662993 14.267408462544367 38 28.09962970317848 40.33285829429836 17.201864333524128 14.36564766899903 39 19.61051253885526 39.37147965503541 17.66103021945918 23.356977586650153 40 19.18293239150011 39.66283462172688 26.239956962587858 14.914276024185154 41 18.41557347806507 40.190545258885386 25.59552704069995 15.79835422234959 42 24.70774045421974 39.27494479460669 19.692312191983152 16.32500255919042 43 18.15849069853178 38.266807890594364 27.284404313961858 16.290297096911992 44 18.15548553020794 38.425482984718286 19.52712409408364 23.891907390990134 45 18.124959757432435 38.580769028149305 27.1837812209156 16.110489993502654 46 17.49016920964252 38.58266794327861 20.270313794729 23.656849052349873 47 18.81874932257658 46.150916755280974 19.802148420907702 15.228185501234742 48 18.92876614354639 46.12613510455566 19.296924005885835 15.648174746012112 49 20.011954082260495 46.26640023908165 17.97536521479259 15.74628046386527 50 26.089824783618326 39.76438439706331 18.34406445711069 15.80172636220768 51 19.258872804330814 47.26056104788339 18.50180108622059 14.978765061565202 52 19.352531062911556 39.95226954222152 25.387736673292636 15.307462721574282 53 19.011082444001406 39.70740002345491 25.46294124545561 15.818576287088074 54 19.123004511731388 47.029259774679346 17.912785011715137 15.934950701874126 55 18.540507402181788 47.4320427792428 17.59481157949389 16.432638239081527 56 25.555219932490886 40.15941769724128 17.111595320697614 17.173767049570223 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 13.5 4 42.0 5 57.0 6 73.0 7 89.0 8 474.0 9 859.0 10 1004.0 11 1149.0 12 1044.5 13 791.0 14 642.0 15 693.0 16 744.0 17 744.0 18 744.0 19 814.5 20 885.0 21 1065.5 22 1638.0 23 2030.0 24 2677.5 25 3325.0 26 4079.5 27 4834.0 28 6987.5 29 12478.5 30 15816.0 31 17905.0 32 19994.0 33 19674.5 34 19355.0 35 16519.5 36 13684.0 37 12099.0 38 9665.0 39 8816.0 40 8173.0 41 7530.0 42 8263.5 43 8997.0 44 20891.0 45 32785.0 46 21533.5 47 10213.5 48 10145.0 49 11428.5 50 12712.0 51 13267.0 52 13822.0 53 15537.5 54 17669.5 55 18086.0 56 18508.0 57 18930.0 58 18655.0 59 18380.0 60 16823.5 61 15267.0 62 13720.0 63 10606.5 64 9040.0 65 8074.5 66 7109.0 67 6000.0 68 4891.0 69 4307.0 70 3723.0 71 3267.0 72 2526.0 73 2241.0 74 1749.5 75 1258.0 76 987.5 77 717.0 78 605.5 79 392.0 80 290.0 81 201.0 82 112.0 83 97.0 84 82.0 85 49.0 86 16.0 87 11.5 88 6.0 89 5.0 90 2.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03226284628061701 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0014532813639917572 21 0.0 22 0.0 23 0.0 24 0.008719688183950542 25 0.02063659536868295 26 0.06568831765242741 27 0.06597897392522577 28 0.1052175707530032 29 0.1685806382230438 30 0.24792980069699372 31 0.28687774125197285 32 0.289493647707158 33 0.31449008716781623 34 0.33454536999090245 35 0.38250365500263045 36 0.42116093928481113 37 0.423486189467198 38 0.4711538182061276 39 0.50952044621551 40 0.5874163273254682 41 0.605437016238966 42 0.6231670488796655 43 0.6612430206162494 44 0.659208426706661 45 0.6891460228048911 46 0.7324538074518455 47 0.7795401236451784 48 0.8402872846600339 49 0.7972701562858779 50 0.8676089743030789 51 0.8330208778400751 52 0.8623771613927087 53 0.8629584739383053 54 1.627093815125171 55 0.9114980714956299 56 0.8888268822173586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 344049.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.28851462212655 #Duplication Level Percentage of deduplicated Percentage of total 1 73.78236088128278 28.25017003458014 2 12.372396804254345 9.474413919008887 3 5.007870015371911 5.752317129278296 4 2.6077417420848708 3.993862312901854 5 1.4781532308673084 2.829814579690327 6 0.9589657475417824 2.2030424428123236 7 0.664876649401278 1.781999751875794 8 0.46131395080851606 1.4130420760738267 9 0.34256132115786275 1.1804547738715037 >10 2.012967617627054 14.424786545245855 >50 0.1777034776315004 4.674494278569747 >100 0.10964682662369174 8.187965202722356 >500 0.014367515212759606 3.969483837670563 >1k 0.008318035123176614 5.311464697451967 >5k 0.0 0.0 >10k+ 7.561850111978742E-4 6.552688418246548 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 22632 6.578132765972288 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 2850 0.8283703774753015 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 2243 0.6519420198867022 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 2069 0.6013678284197891 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 1952 0.5673610445023819 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1523 0.44266950347188916 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 1471 0.42755537728637494 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAA 1470 0.42726472101357654 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 1365 0.3967458123697497 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 1254 0.36448296608913267 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAA 1125 0.32698830689814534 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 1023 0.29734136707271347 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAA 934 0.27147295879366024 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAA 933 0.27118230252086184 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAA 876 0.2546148949713558 No Hit ATGCTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 866 0.2517083322433723 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 812 0.23601289351226135 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 809 0.23514092469386627 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAA 779 0.22642123650991575 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA 769 0.2235146737819322 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAA 719 0.20898186014201464 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAA 699 0.20316873468604765 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAA 680 0.19764626550287895 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAA 675 0.1961929841388872 No Hit ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 671 0.1950303590476938 No Hit ATGCTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 652 0.1895078898645251 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAA 636 0.1848573894997515 No Hit ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 630 0.18311345186296138 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 536 0.15579176221991636 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAA 520 0.15114126185514273 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 514 0.14939732421835264 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 488 0.14184026112559547 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAA 482 0.14009632348880538 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAA 482 0.14009632348880538 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAA 445 0.1293420413952664 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAA 435 0.12643547866728286 No Hit ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA 427 0.12411022848489604 Illumina Single End Adapter 1 (95% over 21bp) ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAA 426 0.1238195722120977 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAA 418 0.12149432202971089 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAA 417 0.12120366575691253 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAA 417 0.12120366575691253 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 415 0.12062235321131581 No Hit ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 400 0.11626250911934055 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAA 379 0.11015872739057518 No Hit ATGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 362 0.1052175707530032 No Hit ATGCTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 355 0.10318297684341474 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAA 353 0.10260166429781806 No Hit ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 345 0.10027641411543124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.001453281363991757 0.0 0.0 0.0 0.0 8 0.002325250182386811 0.0 0.0 0.0 0.0 9 0.004359844091975271 0.0 0.0 0.0 0.0 10 0.022380533005473057 0.0 0.0 0.0 0.0 11 0.04388909719255106 0.0 0.0 0.0 0.0 12 0.05464337928609006 0.0 0.0 0.0 0.0 13 0.06452569256123401 0.0 0.0 0.0 0.0 14 0.07847719365555487 0.0 0.0 0.0 0.0 15 0.08777819438510212 0.0 0.0 0.0 0.0 16 0.09795116393304443 0.0 0.0 0.0 0.0 17 0.10638019584419661 0.0 0.0 0.0 0.0 18 0.11393725893695375 0.0 0.0 0.0 0.0 19 0.12062235321131583 0.0 0.0 0.0 0.0 20 0.1374804170336202 0.0 0.0 0.0 0.0 21 0.15055994930954603 0.0 0.0 0.0 0.0 22 0.16887129449584215 0.0 0.0 0.0 0.0 23 0.18979854613732347 0.0 0.0 0.0 0.0 24 0.21334170423398993 0.0 0.0 0.0 0.0 25 0.2284558304195042 0.0 0.0 0.0 0.0 26 0.24415126915061516 0.0 0.0 0.0 0.0 27 0.25984670788172615 0.0 0.0 0.0 0.0 28 0.28774971007036787 0.0 0.0 0.0 0.0 29 0.29821333589110854 0.0 0.0 0.0 0.0 30 0.31710599362300135 0.0 0.0 0.0 0.0 31 0.32698830689814534 0.0 0.0 0.0 0.0 32 0.3438463707204497 0.0 0.0 0.0 0.0 33 0.35430999654119033 0.0 0.0 0.0 0.0 34 0.36913346645390627 0.0 0.0 0.0 0.0 35 0.3990710625521365 0.0 0.0 0.0 0.0 36 0.41302256364645734 0.0 0.0 0.0 0.0 37 0.4252301271039881 0.0 0.0 0.0 0.0 38 0.43627506547032546 0.0 0.0 0.0 0.0 39 0.44877328520065457 0.0 0.0 0.0 0.0 40 0.47057250566053094 0.0 0.0 0.0 0.0 41 0.492953038666004 0.0 0.0 0.0 0.0 42 0.5147522591258803 0.0 0.0 0.0 0.0 43 0.5443991989513122 0.0 0.0 0.0 0.0 44 0.5792779516871144 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCATAC 15 0.008294968 50.198593 40 CACCATA 15 0.008304584 50.183884 39 GCCTATT 15 0.008362455 50.095802 35 GCCCCTA 15 0.008367291 50.088478 34 TAGCCTA 15 0.008401202 50.037254 33 ACATACC 15 0.008401202 50.037254 33 GACATAC 15 0.008410909 50.02264 32 AATTTAG 15 0.008459573 49.949688 29 CCATCGG 25 2.105894E-5 49.913292 27 AGACGTG 15 0.0084937615 49.898746 24 CACCACG 15 0.0084937615 49.898746 24 GCGGCAT 15 0.008547694 49.81891 21 TAGGCAG 15 0.008557527 49.80442 6 CGTCGCA 15 0.008572293 49.782703 16 CGGTATT 15 0.008572293 49.782703 15 TGGGTAC 2860 0.0 48.49836 6 GATTAAG 2555 0.0 48.443264 50 TAGCATA 2640 0.0 48.362617 30 CATATGC 2640 0.0 48.331436 33 GCATATG 2645 0.0 48.225983 32 >>END_MODULE