FastQCFastQC Report
Fri 17 Jun 2016
SRR1526906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27507
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1640.5962118733413313No Hit
GTCGGTGGGAGAGTCACTCACTCCATCACCGGGAAATATGCAAGCATAGTTTACAAGATAATAG870.3162831279310721No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.2581161158977715No Hit
GTCGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.19631366561238955No Hit
GTCGGTGGGATTTATTTATTTATATTTCTTTCATATTTTCTTAGTTGAGCATCACAAGCTCATA450.163594721343658No Hit
GTCGGTGGGAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCCTTGTGGGGT390.14178209183117024No Hit
GTCGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.13451121532700766No Hit
GTCGGTGGGAGTCTTATTTATGCTCCCCCTCCCTCTTTTCCTCATGCCAAAGTGTTCTTAGTGG370.13451121532700766No Hit
GTCGGTGGGATGAAGGAAATCCTAAGGTTCAATGTTCAAGAGAACGGGTTTTTATCTGCATTGC300.10906314756243865No Hit
GTCGGTGGGATTTTCAGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATA300.10906314756243865No Hit
GTCGGTGGGTGTCTTTGATCCACTTGGACTTGAGTTTTGTGCAGGGTGATAAATATGGATCTAT290.10542770931035736No Hit
GTCGGTGGGTATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGG280.10179227105827607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCAA150.00450881458.41651557
AATCAAA150.00450881458.41651558
TCGGTAG150.00460815258.0967182
GGGACTA150.00460815258.0967187
GGGATTC150.00460815258.0967187
TGGGTGG150.00460815258.0967186
TGGGCAT150.00460815258.0967186
GGTGGAT150.00460815258.0967184
TGGGATC550.058.0967186
CGGTGGC201.9595766E-458.0967143
CGGTGAG258.363844E-658.0967143
TGGGATT406.657501E-1058.0967146
GCCCTAC150.004641623757.99089426
CTACACA150.004641623757.99089429
GGAGAGT150.004641623757.9908948
GTTTGCG150.004641623757.99089451
TATATAA150.004641623757.99089452
CCTGCCA303.631285E-757.99089422
CCTGTAC150.004641623757.99089440
GGACTAA150.004641623757.9908948
Produced by FastQC (version 0.11.5)