FastQCFastQC Report
Fri 17 Jun 2016
SRR1526905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42190
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG10572.505333017302678No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5551.3154776013273286No Hit
GTCGGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC3570.8461720786916331No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1720.40767954491585684No Hit
GTCGGTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1490.3531642569329225No Hit
GTCGGTGGGAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCCTTGTGGGGT1230.2915382792130837No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.27494666982697324No Hit
GTCGGTGGGAGACTCAGCACCTTGCCCATGCCAGGCATTGCACTACTGCTGAGCTAACTCACGA1140.2702062100023702No Hit
GTCGGTGGGAGAGTCACTCACTCCATCACCGGGAAATATGCAAGCATAGTTTACAAGATAATAG1070.2536146006162598No Hit
GTCGGTGGGGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTC950.22517184166864188No Hit
GTCGGTGGGGGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTGAGCGCTAGT820.19435885280872245No Hit
GTCGGTGGGGGCAGGCTCTGTGCGCCTACCAAGCAGAGCCAAACACCTGTGCCGCCGCACAGGA820.19435885280872245No Hit
GTCGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.19435885280872245No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTAT660.15643517421189854No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT650.15406494429959708No Hit
GTCGGTGGGATTCCTGGAGTTCCTGTCCAGCCCGTGGTGCTACGCTACCCAAACAAATTGGACA630.14932448447499408No Hit
GTCGGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC610.14458402465039108No Hit
GTCGGTGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCGGACAGCG610.14458402465039108No Hit
GTCGGTGGGTGTCTTTGATCCACTTGGACTTGAGTTTTGTGCAGGGTGATAAATATGGATCTAT600.1422137947380896No Hit
GTCGGTGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC580.13747333491348662No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC560.13273287508888362No Hit
GTCGGTGGGGATGCTCTGTCCTCTCCACACAGGCCTCTTGCTCCTAACAGCTCAGCCCTGGCCT560.13273287508888362No Hit
GTCGGTGGGAACCTTCTCTAACCCACGATATCAAGACCCAGAAACTGAAGAAAAATCTTGTTTT550.13036264517658214No Hit
GTCGGTGGGGTGCCAGCCTTGAGTGGGAGACATACAGGGCAGTCAGATCCTGCTCCAAATTCTC540.12799241526428062No Hit
GTCGGTGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCCTGGCTC530.12562218535197914No Hit
GTCGGTGGGATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGGCTGGCT530.12562218535197914No Hit
GTCGGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG530.12562218535197914No Hit
GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGGGAGCAAAGAG500.11851149561507467No Hit
GTCGGTGGGATCGGTTGGCTGCAAAGTCCTTGATTCAGACTAAGGATTTTGGATCCCAGGAGGC490.11614126570277317No Hit
GTCGGTGGGGAGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCA480.11377103579047168No Hit
GTCGGTGGGGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACATAA460.10903057596586868No Hit
GTCGGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT450.10666034605356721No Hit
GTCGGTGAGGGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCCCGTGCTCTG440.10429011614126571No Hit
GTCGGTGGGGAAACACTAGAATTTATTTATATGTATTGATGTTGTAGGTCTAGATGGAAAAAAA440.10429011614126571No Hit
GTCGGTGGGACCCACTGCTGGTGGTTGTTTCCTGCCTTACCCGGGACCCCTTCAGCAGCAGTTT430.10191988622896421No Hit
GTCGGTGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC430.10191988622896421No Hit
GTCGGTGGGATCCTTAATCATCAGGGAAATGCAAATCAAAACAACCCTGAAATTCCACCTCACA430.10191988622896421No Hit
GTCGGTGGGAGTGAAGGGGCCAGGCAGGGCATTTGTGCCAAGTATAAGAATCAGCAGACACCCA430.10191988622896421No Hit
GTCGGTGGGGAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCCC430.10191988622896421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTAAC201.968403E-458.1166752
TAACTCA201.968403E-458.1166755
TAGACTG201.968403E-458.1166744
CCGTGCT150.00461902358.11666555
TAATTGC150.00461902358.11666552
CAAAGCA150.00461902358.11666547
AAAGCAG150.00461902358.11666548
CGCACAG150.00461902358.11666556
CGCTGTT150.00461902358.11666555
TGCACTA201.9800407E-458.04756539
GTAGACT201.9800407E-458.04756543
TTGCATT258.484532E-658.04756539
ATTAGTA201.9800407E-458.04756539
AGTAGAC201.9800407E-458.04756542
CACTACT201.9800407E-458.04756541
CTGCCCG201.9800407E-458.04756551
GCACTAC201.9800407E-458.04756540
TTAGTAG201.9800407E-458.04756540
TTGCCTT201.9800407E-458.04756551
AGGACCA150.004640869358.0475640