FastQCFastQC Report
Fri 17 Jun 2016
SRR1526904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42223
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG10082.3873244440234No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5361.2694502995997443No Hit
GTCGGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC3410.8076167017975984No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1750.414466049309618No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.3031523103521777No Hit
GTCGGTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1250.29604717807829856No Hit
GTCGGTGGGAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCCTTGTGGGGT1090.2581531392842763No Hit
GTCGGTGGGAGAGTCACTCACTCCATCACCGGGAAATATGCAAGCATAGTTTACAAGATAATAG1050.2486796295857708No Hit
GTCGGTGGGGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTC1010.23920611988726523No Hit
GTCGGTGGGAGACTCAGCACCTTGCCCATGCCAGGCATTGCACTACTGCTGAGCTAACTCACGA1000.23683774246263883No Hit
GTCGGTGGGATTCCTGGAGTTCCTGTCCAGCCCGTGGTGCTACGCTACCCAAACAAATTGGACA730.17289155199772638No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT720.17052317457309998No Hit
GTCGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.1681547971484736No Hit
GTCGGTGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC690.1634180422992208No Hit
GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGGGAGCAAAGAG690.1634180422992208No Hit
GTCGGTGGGGGCAGGCTCTGTGCGCCTACCAAGCAGAGCCAAACACCTGTGCCGCCGCACAGGA630.1492077777514625No Hit
GTCGGTGGGGAGCCCTTTCTCCTCTACTCGCAAAGATGACGCTTTGCCGTCCTGGCCCTGCGGC600.1421026454775833No Hit
GTCGGTGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCGGACAGCG600.1421026454775833No Hit
GTCGGTGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCCTGGCTC590.1397342680529569No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTAT590.1397342680529569No Hit
GTCGGTGGGTGTCTTTGATCCACTTGGACTTGAGTTTTGTGCAGGGTGATAAATATGGATCTAT580.13736589062833054No Hit
GTCGGTGGGATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGGCTGGCT580.13736589062833054No Hit
GTCGGTGGGCTGTCGGTGGGGGAGAAAGTGTGCAAATACCAACGCCTCTCTTTGTCTGCCACCT570.13499751320370415No Hit
GTCGGTGGGGGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTGAGCGCTAGT530.1255240035051986No Hit
GTCGGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC500.11841887123131942No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC490.11605049380669302No Hit
GTCGGTGGGAGATCCGTAAAAGAATGAGCTGTCAACAAATGGTGCTAGAAACATTGATTATCCA480.11368211638206666No Hit
GTCGGTGGGGATGCTCTGTCCTCTCCACACAGGCCTCTTGCTCCTAACAGCTCAGCCCTGGCCT480.11368211638206666No Hit
GTCGGTGGGACCCACTGCTGGTGGTTGTTTCCTGCCTTACCCGGGACCCCTTCAGCAGCAGTTT470.11131373895744026No Hit
GTCGGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG470.11131373895744026No Hit
GTCGGTGGGAACCTTCTCTAACCCACGATATCAAGACCCAGAAACTGAAGAAAAATCTTGTTTT460.10894536153281387No Hit
GTCGGTGGGAGTGAAGGGGCCAGGCAGGGCATTTGTGCCAAGTATAAGAATCAGCAGACACCCA450.10657698410818747No Hit
GTCGGTGGGGAGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCA440.10420860668356108No Hit
GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG440.10420860668356108No Hit
GTCGGTGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC430.10184022925893471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGC150.004515127358.4508443
AAGTATA258.142226E-658.4508442
TTGTCGG201.9132372E-458.4508440
TATACCC258.142226E-658.4508445
GCTAGAA150.004515127358.4508444
ATGGTGC150.004515127358.4508439
GAATGCC201.9132372E-458.4508445
TTAAGCA150.004515127358.4508445
TGCTAGA150.004515127358.4508443
GAAAGTA258.142226E-658.4508440
GGAAAGT258.142226E-658.4508439
TCGGGAA201.9132372E-458.4508443
GTGCTAG150.004515127358.4508442
ACAGCCT258.200437E-658.3808422
TACCTCG201.9246314E-458.3808425
TACTTAC201.9246314E-458.3808421
CGCTGAC150.004536637658.38083623
TCGGTTC150.004536637658.38083626
ACTTAGC150.004536637658.38083620
GCTGACC150.004536637658.38083624
Produced by FastQC (version 0.11.5)