FastQCFastQC Report
Fri 17 Jun 2016
SRR1526903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38940
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA8342.14175654853621No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5101.3097072419106317No Hit
GTCGGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG3150.8089368258859784No Hit
GTCGGTGGGGGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTGA1300.3338469440164355No Hit
GTCGGTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC1270.3261427837699024No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1230.31587057010785824No Hit
GTCGGTGGGAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCCT1170.30046224961479195No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1150.2953261427837699No Hit
GTCGGTGGGGAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT1100.2824858757062147No Hit
GTCGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.26707755521314847No Hit
GTCGGTGGGGCACCCGCTTCACCGACGACTACCAGCTTTTCGAGGAGCTCGGCAAG990.2542372881355932No Hit
GTCGGTGGGGGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTTTG950.24396507447354907No Hit
GTCGGTGGGAGAGTCACTCACTCCATCACCGGGAAATATGCAAGCATAGTTTACAA940.241397021058038No Hit
GTCGGTGGGAGACTCAGCACCTTGCCCATGCCAGGCATTGCACTACTGCTGAGCTA910.23369286081150487No Hit
GTCGGTGGGGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGT760.19517205957883924No Hit
GTCGGTGGGAACCTTCTCTAACCCACGATATCAAGACCCAGAAACTGAAGAAAAAT720.18489984591679506No Hit
GTCGGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC680.1746276322547509No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC670.17205957883923986No Hit
GTCGGTGGGATTCCTGGAGTTCCTGTCCAGCCCGTGGTGCTACGCTACCCAAACAA660.1694915254237288No Hit
GTCGGTGGGGGCAGGCTCTGTGCGCCTACCAAGCAGAGCCAAACACCTGTGCCGCC660.1694915254237288No Hit
GTCGGTGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCT600.15408320493066258No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC550.14124293785310735No Hit
GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGGGA550.14124293785310735No Hit
GTCGGTGGGATCGGTTGGCTGCAAAGTCCTTGATTCAGACTAAGGATTTTGGATCC510.13097072419106318No Hit
GTCGGTGGGCTCTAGTTTGTTGTTGATGGGTTGTTTTCTGAAACAGGGTCTCATCA510.13097072419106318No Hit
GTCGGTGGGACCCACTGCTGGTGGTTGTTTCCTGCCTTACCCGGGACCCCTTCAGC510.13097072419106318No Hit
GTCGGTGGGATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGT500.12840267077555212No Hit
GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG500.12840267077555212No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC490.1258346173600411No Hit
GTCGGTGGGGCCTGGGAAGAACGTGCAGCTGACAGAGAACGAGATCCGTGGTCTGT490.1258346173600411No Hit
GTCGGTGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT480.12326656394453005No Hit
GTCGGTGGGGATGCTCTGTCCTCTCCACACAGGCCTCTTGCTCCTAACAGCTCAGC450.11556240369799693No Hit
GTCGGTGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG450.11556240369799693No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC450.11556240369799693No Hit
GTCGGTGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGC450.11556240369799693No Hit
GTCGGTGGGGTCTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC440.11299435028248588No Hit
GTCGGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA430.11042629686697483No Hit
GTCGGTGGGGAGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA430.11042629686697483No Hit
GTCGGTGGGGAACTACCAGCCTCCCACTGTGGTACCCGGTGGTGACCTGGCCAAGG420.10785824345146379No Hit
GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGG420.10785824345146379No Hit
GTCGGTGGGGAGCCCTTTCTCCTCTACTCGCAAAGATGACGCTTTGCCGTCCTGGC410.10529019003595276No Hit
GTCGGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT400.1027221366204417No Hit
GTCGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA390.10015408320493066No Hit
GTCGGTGGGATGAAGGAAATCCTAAGGTTCAATGTTCAAGAGAACGGGTTTTTATC390.10015408320493066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTGA150.00802570850.5332550
TTCCTCA150.00802570850.5332550
TCTTGTG150.00802570850.5332549
CTTGTGC150.00802570850.5332550
GCATATT150.00802570850.5332549
GCCCTGT150.00802570850.5332548
TGCGCCC150.00823491250.2059642
TATAAGC150.00823491250.2059634
ACGAGAT150.00823491250.2059639
TCAGACT252.0006148E-550.2059635
GAATCTC150.00823491250.2059639
AGAGAAC150.00823491250.2059634
ATATGCT1000.050.2059634
CAGACTA150.00823491250.2059636
GCCGCAT150.00823491250.2059640
GCGTAGA309.929936E-750.2059638
CAGCGTA309.929936E-750.2059636
AAGCCTG150.00823491250.2059637
TTCAGAC204.050458E-450.2059634
GCCTGCG150.00823491250.2059639