##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526903_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38940 Sequences flagged as poor quality 0 Sequence length 56 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.572573189522345 38.0 38.0 38.0 35.0 39.0 2 37.308834103749355 38.0 38.0 38.0 35.0 39.0 3 37.36705187467899 38.0 38.0 38.0 35.0 39.0 4 37.3552645095018 38.0 38.0 38.0 35.0 39.0 5 37.26700051361068 38.0 38.0 38.0 35.0 39.0 6 35.786774524910115 37.0 35.0 39.0 31.0 39.0 7 38.07455059065229 38.0 38.0 39.0 36.0 39.0 8 38.02131484334874 38.0 38.0 39.0 36.0 39.0 9 37.10511042629687 38.0 35.0 39.0 32.0 39.0 10 36.047457627118646 38.0 35.0 39.0 31.0 39.0 11 35.577606574216745 38.0 35.0 39.0 30.0 39.0 12 35.825115562403695 38.0 35.0 39.0 30.0 39.0 13 35.81276322547509 38.0 35.0 39.0 31.0 39.0 14 36.186902927580896 38.0 35.0 39.0 31.0 39.0 15 36.267205957883924 38.0 35.0 39.0 31.0 39.0 16 36.358936825885976 38.0 35.0 39.0 32.0 39.0 17 36.21715459681561 38.0 35.0 39.0 31.0 39.0 18 36.13138161273754 38.0 35.0 39.0 31.0 39.0 19 36.079635336414995 38.0 35.0 39.0 31.0 39.0 20 35.743656908063684 38.0 35.0 39.0 30.0 39.0 21 35.80215716486903 38.0 35.0 39.0 30.0 39.0 22 35.52835130970724 38.0 35.0 39.0 30.0 39.0 23 35.41972265023112 38.0 35.0 39.0 29.0 39.0 24 35.01101694915254 38.0 34.0 39.0 29.0 39.0 25 34.90991268618387 37.0 33.0 39.0 29.0 39.0 26 34.200025680534154 37.0 33.0 38.0 27.0 39.0 27 33.82799178222907 36.0 33.0 38.0 26.0 39.0 28 33.51908063687725 36.0 33.0 38.0 25.0 39.0 29 33.39013867488444 36.0 32.0 38.0 25.0 39.0 30 33.03076527991782 36.0 32.0 38.0 23.0 39.0 31 32.266897791474065 35.0 31.0 38.0 20.0 39.0 32 31.94809964047252 35.0 30.0 38.0 18.0 39.0 33 31.527966101694915 35.0 30.0 38.0 17.0 39.0 34 31.815100154083204 35.0 30.0 38.0 18.0 39.0 35 31.297123780174626 35.0 30.0 38.0 14.0 39.0 36 31.250410888546483 36.0 30.0 38.0 4.0 39.0 37 30.848921417565485 35.0 30.0 38.0 4.0 39.0 38 30.367103235747305 35.0 29.0 38.0 4.0 39.0 39 30.345557267591165 35.0 29.0 38.0 4.0 39.0 40 29.99827940421161 35.0 29.0 38.0 4.0 39.0 41 29.395608628659478 35.0 28.0 38.0 4.0 39.0 42 29.264586543400103 35.0 27.0 38.0 4.0 39.0 43 28.92668207498716 34.0 27.0 38.0 4.0 39.0 44 28.60683102208526 34.0 26.0 38.0 4.0 39.0 45 28.254827940421162 34.0 25.0 38.0 4.0 39.0 46 27.598741653826398 33.0 23.0 38.0 4.0 39.0 47 27.237313816127376 33.0 21.0 37.0 4.0 39.0 48 26.843502824858756 33.0 18.0 37.0 4.0 39.0 49 26.510066769388803 33.0 16.0 37.0 4.0 39.0 50 25.967411402157165 33.0 4.0 36.0 4.0 39.0 51 25.05179763739086 32.0 4.0 36.0 4.0 39.0 52 24.729635336414997 32.0 4.0 36.0 4.0 39.0 53 24.32914740626605 32.0 4.0 36.0 4.0 39.0 54 23.384591679506933 31.0 4.0 36.0 4.0 39.0 55 23.325885978428353 31.0 4.0 36.0 4.0 39.0 56 13.332331792501284 16.0 4.0 21.0 4.0 24.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.16380090586715568 1 2 -0.2977180438503737 1 3 -0.12722752126340708 1 4 -0.37244877425515455 1 5 -0.025139451718381167 1 6 0.16223432886842204 1 7 -0.2583097146586937 1 8 -0.08883818982339164 1 9 -0.0991328598182335 1 10 -0.32514380313082114 1 11 -0.7681286454186775 1 12 -0.7093320976571817 1 13 0.6358030809385866 1 14 -0.2083525345303201 1 15 -0.09884294547133265 1 16 0.16501281882941754 1 17 0.822805286363355 1 18 0.2025720339897248 1 19 0.4690475660597997 1 20 1.010579868536901 1 21 0.3683686762339349 1 22 0.19086749933298108 1 23 0.09362021863830705 1 24 1.1051643182110524 1 25 -0.23257662848159555 1 26 1.4377731789863262 1 27 -0.07587085550130723 1 28 1.7715458164569213 1 29 1.3784091611280544 1 30 2.5066787635807515 1 31 1.5225546691323402 1 32 1.6118003337340632 1 33 1.7406808619422556 1 34 -0.06983558685974245 1 35 0.15830098403552384 1 36 2.7682212613190913 1 37 1.8114604565967625 1 38 1.5869377602963652 1 39 1.9300745612958679 1 40 1.6547511072349863 1 41 3.3031438246207543 1 42 1.9581533178397805 1 43 4.422697454552942 1 44 2.9582591209335583 1 45 2.776596588517993 1 46 3.7263858248144643 1 47 3.475023126989065 1 48 2.768571163999887 1 49 3.4195022106836035 1 50 3.218437415580116 1 51 2.236710228402938 1 52 4.0254614184348085 1 53 3.4581740021375715 1 54 2.7980996089055985 1 55 2.010621005291945 1 56 0.6352390330889843 2 1 -0.230793688727438 2 2 -0.08459063072295692 2 3 0.048062054026168255 2 4 -0.15932136112773776 2 5 -0.016645243224168382 2 6 0.02555479218888479 2 7 0.2899528336038557 2 8 0.10343980245460216 2 9 -0.23581239649777075 2 10 0.06558978760276801 2 11 -0.5411016183916502 2 12 -0.33558692391201106 2 13 -0.28234363720813604 2 14 -0.6423293685071556 2 15 -0.21467306130145403 2 16 0.5696460234626244 2 17 -0.04823718467911675 2 18 0.9709118023294963 2 19 0.058236755248984196 2 20 0.2121242700813042 2 21 0.8170173248825776 2 22 -1.0384761300106433 2 23 0.3886009136190012 2 24 1.4773650904118227 2 25 1.0832534873485216 2 26 0.8122905535037006 2 27 -0.35463533426578664 2 28 0.8433604882715926 2 29 1.6177914005102991 2 30 0.48814594504793263 2 31 0.6553732019508729 2 32 1.1021478240815519 2 33 0.33218665344804776 2 34 0.41278989576574077 2 35 0.3590731848077269 2 36 0.05702435012218032 2 37 0.5226573677936734 2 38 1.5197562931148987 2 39 0.30381973504103854 2 40 0.4485735010573819 2 41 -0.527744206267279 2 42 -0.03875787907141515 2 43 0.8821569140124019 2 44 -0.10660574393130418 2 45 -0.12378951186810738 2 46 1.3495518479804858 2 47 0.8873783393442771 2 48 1.0218530172817424 2 49 2.3345601257415183 2 50 1.0323370294797307 2 51 2.0745480662407765 2 52 1.6239170168904096 2 53 2.6272859712495418 2 54 1.4575590683650574 2 55 2.28552448019542 2 56 2.005123202973154 3 1 -0.3028657607995058 3 2 -0.40614295227528174 3 3 -0.20488319891908446 3 4 -0.8142070160133983 3 5 -0.5218427484216761 3 6 -0.6612088945748056 3 7 -0.24365770000667908 3 8 -0.4675927685779726 3 9 -0.4353965960819721 3 10 -1.455546733533751 3 11 -1.1065901838802148 3 12 -0.948892537217624 3 13 -0.10632146118595642 3 14 -0.21128293746072302 3 15 -0.24170008832847856 3 16 0.9584194122360117 3 17 0.04185290541097686 3 18 0.7630115944292868 3 19 0.5869962840085137 3 20 0.16222822018524852 3 21 0.8863173941826474 3 22 0.7300616385271255 3 23 0.6533271783452648 3 24 -0.39373678069004825 3 25 1.007716411811444 3 26 0.22531896653211447 3 27 -0.1176290972595524 3 28 0.6770403219514307 3 29 1.268519051237945 3 30 0.39459085149284334 3 31 1.3386718852495534 3 32 1.7986135205472458 3 33 0.863025184286581 3 34 0.563864046839889 3 35 0.2059200316545713 3 36 -0.6581523650545371 3 37 0.26347510861141643 3 38 0.12613189949050252 3 39 -0.6941012628799612 3 40 -0.5826115301276502 3 41 -0.4272591057821771 3 42 0.19478335446981632 3 43 0.9611589930144824 3 44 0.5531309158053546 3 45 0.6835562954777004 3 46 0.8831623815910206 3 47 0.8728253247912647 3 48 0.9414649368936594 3 49 0.9762787674601583 3 50 1.8396828368255385 3 51 2.5444025360952445 3 52 3.6481720411454326 3 53 1.4200787640423336 3 54 3.1263047371107255 3 55 3.121976316647256 3 56 0.8755320733820255 4 1 0.021695642709261165 4 2 -0.012755637835333289 4 3 0.29984014790952074 4 4 0.017776789654618597 4 5 -0.23641764720710512 4 6 -0.840021310229325 4 7 0.3319158492510823 4 8 0.44198887784578034 4 9 0.4775588695050743 4 10 0.5086907833353465 4 11 0.6950292493181678 4 12 1.0996904587337895 4 13 0.8079835320664017 4 14 0.21044445795087796 4 15 1.2056683327241515 4 16 1.0454639466489652 4 17 0.34009851944605884 4 18 1.0484366956438578 4 19 0.26378440816505844 4 20 -0.3134802818390412 4 21 0.4578423604444595 4 22 -0.051989643524159135 4 23 0.9981314968337927 4 24 1.4645628144516536 4 25 0.24185946174396378 4 26 1.4452919759788116 4 27 0.18803891893477953 4 28 1.2053804029230903 4 29 0.35961216864685497 4 30 1.9796110944078222 4 31 0.2954869999594081 4 32 0.8937552209521051 4 33 0.7361695837467686 4 34 0.06926215750115716 4 35 -0.02215014378402458 4 36 -0.04681633266587326 4 37 -1.0050808967866942 4 38 0.1335543016497489 4 39 0.5812023808447364 4 40 -0.08058723862967554 4 41 1.6159257795079718 4 42 1.0942435434036888 4 43 1.6069079808687334 4 44 1.0740485946177678 4 45 -0.3279146896774954 4 46 1.9917993586490503 4 47 1.1299103450341796 4 48 0.8490222918194377 4 49 1.8480736392550305 4 50 2.6440013253545516 4 51 1.8156575968239892 4 52 1.360047884600224 4 53 1.7709559570247926 4 54 1.7612575036424403 4 55 2.4647563436378093 4 56 0.8991488075250746 5 1 -0.018552035309312487 5 2 0.14513909900676936 5 3 -0.35495861370038284 5 4 0.12923189801375656 5 5 -0.4856436533990518 5 6 0.486604076767577 5 7 0.5610180164337493 5 8 0.6153634908488783 5 9 0.16641335866916052 5 10 0.22695703720530958 5 11 0.5201066486989703 5 12 -0.23622285395970266 5 13 -0.08520532242275891 5 14 1.2630760368982479 5 15 0.40535873520093446 5 16 0.35660945748487904 5 17 1.2967548661952861 5 18 -0.2890246046657339 5 19 0.8690475660597983 5 20 0.38621012063774174 5 21 0.22719220564570008 5 22 0.9185985917699568 5 23 1.1095866051929306 5 24 0.6967609568665125 5 25 0.7867511026108431 5 26 0.9839916663812858 5 27 0.5022804050028924 5 28 0.6883525391459955 5 29 -0.3989017632416889 5 30 2.104998091311842 5 31 2.4796975262751957 5 32 3.1244053757508645 5 33 3.0953027106817537 5 34 2.5057946652410976 5 35 2.431410227733007 5 36 2.728725462999762 5 37 2.880368019621976 5 38 2.0205512056745185 5 39 2.72671321675805 5 40 4.222818334125744 5 41 0.5199505473098291 5 42 0.3976491161591085 5 43 0.8050503957294133 5 44 0.8464944150512075 5 45 1.7959243196104282 5 46 2.519663135738835 5 47 1.8683004379134367 5 48 2.954285449714174 5 49 2.289250109843266 5 50 3.157933213899444 5 51 4.883769051932351 5 52 4.802772342804559 5 53 4.057333666003117 5 54 4.79221725596442 5 55 4.8610411733591725 5 56 1.5663314700637745 6 1 -1.0185520353093125 6 2 -0.9725079598167596 6 3 -0.7373115548768538 6 4 -0.9884151608097795 6 5 -0.9856436533990518 6 6 -1.807513570291242 6 7 -0.8213349247427217 6 8 -1.1199306267981797 6 9 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2.024544227490324 6 50 -0.07736090374761417 6 51 -1.0574074186558846 6 52 -0.344286480724854 6 53 -1.236783981055705 6 54 -1.5607239205061667 6 55 -0.5213117678172985 6 56 -0.6983744122891657 7 1 -0.093341951275697 7 2 -0.18343232956465982 7 3 -1.0415132355491252 7 4 -0.5153059171123004 7 5 -0.5108537374326687 7 6 -0.26633709970300856 7 7 -0.22973828608726166 7 8 -0.3459810469662514 7 9 -0.7562757169610919 7 10 -0.06800094598796136 7 11 -1.3681286454186719 7 12 -1.3379035262286152 7 13 -0.735625490489987 7 14 -1.0369239631017493 7 15 -0.1559858026141896 7 16 -1.349272895456295 7 17 -1.120051856493788 7 18 -1.054570823153128 7 19 -0.6738095767973462 7 20 0.01057986853690096 7 21 0.9397972476625043 7 22 -0.9234182149527328 7 23 0.23647736149544585 7 24 0.10516431821105243 7 25 -0.40400519991016637 7 26 -0.1050839638708112 7 27 0.8955577159272607 7 28 0.5144029593140615 7 29 0.5212663039852004 7 30 1.935250192152182 7 31 1.0939832405609096 7 32 -0.41677109483736885 7 33 -0.7164619952005999 7 34 0.1587358417116853 7 35 0.8154438411783822 7 36 2.6539355470333774 7 37 1.5257461708824778 7 38 0.8726520460106499 7 39 1.358645989867295 7 40 1.4833225358064155 7 41 2.8745723960493237 7 42 2.5295818892683535 7 43 1.5655545974100846 7 44 2.015401978076415 7 45 1.9765965885179924 7 46 1.8121001105287498 7 47 1.9321659841319239 7 48 2.825714021142744 7 49 2.562359353540746 7 50 3.589865987008686 7 51 3.2652816569743663 7 52 2.511175704149096 7 53 2.001031144994716 7 54 2.512385323191314 7 55 3.810621005291946 7 56 0.23523903308898575 8 1 -0.46477052270427066 8 2 -0.4405751867075196 8 3 -0.5557989498348377 8 4 -0.2867344885408656 8 5 -0.28228230886123384 8 6 -0.6663370997030071 8 7 -0.4011668575158325 8 8 -0.40312390410910837 8 9 -1.2991328598182363 8 10 -0.8965723745593905 8 11 -0.7967000739901025 8 12 -0.25218924051432623 8 13 -0.6784826333471301 8 14 0.24879032261253542 8 15 -1.4131286597570494 8 16 0.022155675972278743 8 17 -0.5771947136366435 8 18 -0.968856537438846 8 19 -0.045238148225912767 8 20 -0.6465629886059574 8 21 -1.1744884666232096 8 22 0.048010356475842286 8 23 -0.5349512099331264 8 24 -1.1805499675032323 8 25 -0.37543377133874145 8 26 -0.9907982495850973 8 27 -0.4758708555013058 8 28 -0.08559704068593277 8 29 -0.8215908388719413 8 30 -0.4361783792763916 8 31 0.1511260977037665 8 32 0.29751461944834645 8 33 0.6835380047993986 8 34 0.4730215559973985 8 35 0.3011581268926662 8 36 0.9110784041762336 8 37 0.7257461708824771 8 38 0.7297949031535076 8 39 0.8443602755815789 8 40 0.1976082500921308 8 41 1.2745723960493223 8 42 1.1295818892683513 8 43 1.5084117402672277 8 44 1.0725448352192721 8 45 1.9194537313751354 8 46 2.8406715391001782 8 47 2.703594555560496 8 48 1.96857116399989 8 49 3.1623593535407437 8 50 3.6755802727229714 8 51 4.179567371260081 8 52 4.62546141843481 8 53 2.5724597164232854 8 54 5.2838138946198825 8 55 4.896335291006231 8 56 2.863810461660414 9 1 0.42094376301001546 9 2 0.4165676704353416 9 3 0.35848676445087335 9 4 0.1418369400305579 9 5 0.40343197685304943 9 6 0.5908057574398455 9 7 0.4559759996270216 9 8 0.5397332387480347 9 9 0.6722957116103316 9 10 0.24628476829774826 9 11 0.6032999260098961 9 12 1.433525045199957 9 13 1.0643745095100101 9 14 0.648790322612534 9 15 0.9297284831000923 9 16 0.9364413902579898 9 17 1.1370910006490718 9 18 0.8311434625611511 9 19 1.8976189946312303 9 20 1.010579868536901 9 21 1.5969401048053626 9 22 1.248010356475838 9 23 1.007905932924018 9 24 1.59087860392534 9 25 2.024566228661257 9 26 1.8377731789863248 9 27 0.8955577159272607 9 28 1.9715458164569242 9 29 1.5784091611280573 9 30 2.3066787635807557 9 31 3.2368403834180555 9 32 3.2975146194483465 9 33 1.8549665762279695 9 34 3.187307270283114 9 35 3.244015269749813 9 36 1.7110784041762344 9 37 2.5257461708824778 9 38 1.6440806174392222 9 39 2.5015031327244373 9 40 1.5404653929492724 9 41 3.3031438246207543 9 42 2.2724390321254937 9 43 2.1941260259815145 9 44 2.4725448352192743 9 45 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3.3100936315705596 115 42 2.2585394182258796 115 43 3.5308055626610475 115 44 2.352853715528152 115 45 2.8762104881318926 115 46 4.8360383344669735 115 47 4.914405366371305 115 48 4.8867178821466055 115 49 2.6048303960117885 115 50 4.870174867317566 115 51 5.371845363538075 115 52 5.326619719593111 115 53 3.7353940793576506 115 54 6.808910419716408 115 55 5.393632588303529 115 56 2.9780961759461277 116 1 0.5026897947560443 116 2 0.6451390990067694 116 3 0.4767407327048474 116 4 0.54263059082421 116 5 0.6597811832022558 116 6 0.4947740114081043 116 7 0.3289918726428951 116 8 0.4683046673194653 116 9 0.4278512671658916 116 10 0.29231651432949235 116 11 0.0985380212479896 116 12 -0.6839352722603564 116 13 0.3167554618909634 116 14 0.9852982591204693 116 15 0.7305221338937429 116 16 -0.7103840065674092 116 17 -0.29148042792235884 116 18 -0.7220311406134456 116 19 -0.297619100606866 116 20 -0.149737591780557 116 21 0.2794797873450463 116 22 -1.3575451990797163 116 23 0.6063186313367197 116 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2.55090258275807 117 44 2.6556950183694568 117 45 2.0681716800930836 117 46 1.139572638001276 117 47 2.257440709406648 117 48 2.5824905779193017 117 49 2.1814069725883627 117 50 1.865323862466564 117 51 4.057223048915759 117 52 3.802018194991586 117 53 2.779053123016695 117 54 3.998099608905598 117 55 3.1988993935703327 117 56 1.4909166887666405 118 1 -0.13619909413284148 118 2 -0.3826386787710092 118 3 -0.6343703784062669 118 4 -0.5684805202868972 118 5 -0.2013299279088585 118 6 -0.9496704330363457 118 7 0.1623252059762308 118 8 -0.5872508882360918 118 9 -0.09992651061188695 118 10 0.15342762544060662 118 11 -0.984795312085339 118 12 -0.406157494482585 118 13 -0.3776889825534795 118 14 -0.15359062976841642 118 15 -1.3805889772173643 118 16 1.0118382156548194 118 17 0.5974084609665269 118 18 0.6668577482754401 118 19 0.8968253438375768 118 20 0.4335957415527716 118 21 1.5850353429005963 118 22 1.7257881342536194 118 23 0.9952075202256054 118 24 1.4908786039253386 118 25 0.3325027365977675 118 26 1.8107890520021996 118 27 1.5725418429113915 118 28 2.016783911695015 118 29 0.5014250341439279 118 30 1.1915993985013884 118 31 0.9241419707196386 118 32 2.349895571829297 118 33 1.8224268936882844 118 34 2.969846952822799 118 35 1.2059200316545748 118 36 2.1944117375095686 118 37 3.968603313739621 118 38 2.677413950772557 118 39 0.038804720026021755 118 40 1.534909837393716 118 41 1.57060414208107 118 42 1.543073952760416 118 43 1.8949196767751637 118 44 2.6471480098224482 118 45 2.5361203980418026 118 46 3.893846142274782 118 47 4.159150111116052 118 48 2.9820632274919525 118 49 4.209184750366141 118 50 5.108913606056305 118 51 3.125599117291827 118 52 1.9580011009744922 118 53 2.7811898751534443 118 54 2.8592107200167085 118 55 1.5130019576728984 118 56 2.950318398168349 119 1 0.16867895464764615 119 2 0.010992757543355935 119 3 0.42050767037422077 119 4 0.25333519787027825 119 5 0.2905399907903288 119 6 0.011711680784792122 119 7 0.17723035502771722 119 8 0.28537783805117556 119 9 0.2137591262444829 119 10 -0.41364554529110364 119 11 0.383090866776449 119 12 0.27812434833584376 119 13 -0.6791794974586267 119 14 0.1045394515323963 119 15 0.8802511311837193 119 16 -0.8614680174075176 119 17 -0.5353828669467475 119 18 -1.0343617639196836 119 19 -1.1065621900377636 119 20 -1.297434068745332 119 21 -0.6608996164489938 119 22 -0.820282326450986 119 23 -0.5711881437310353 119 24 -0.6432677375380749 119 25 -0.4263048514781147 119 26 0.9882958270699547 119 27 1.4621082385753468 119 28 1.5025562694185979 119 29 1.1484440043336335 119 30 0.7735777182845851 119 31 2.2723804531044607 119 32 0.817375246626046 119 33 1.585977029189646 119 34 0.6758090124433949 119 35 0.45795255197977625 119 36 -1.2547752543603536 119 37 -1.1167625399189163 119 38 -1.790065724024192 119 39 -0.7653958219793964 119 40 -0.5863638753434088 119 41 -1.8975530394907452 119 42 -1.3505574835539491 119 43 -0.23583913081291286 119 44 -1.0466189278469287 119 45 -0.7098145613077911 119 46 -0.0011403075897185033 119 47 0.030423823853176657 119 48 0.7957488643483188 119 49 0.8480736392550305 119 50 0.7877753946741919 119 51 2.773295594256595 119 52 3.5968899898633815 119 53 3.037964942904125 119 54 3.022489852808036 119 55 2.389018217835499 119 56 1.9293156881412497 120 1 -0.02508798302172721 120 2 -0.9659720121043449 120 3 -0.5232592672951526 120 4 -0.5407027425091258 120 5 -0.9235521501310799 120 6 -0.06078154414745285 120 7 -0.5321192384682121 120 8 -0.642806443791649 120 9 -1.0721487328341084 120 10 -0.6799057078927291 120 11 -0.09590642319645326 120 12 -0.04504638337147071 120 13 -0.48880009366458665 120 14 -0.9869239631017521 120 15 -1.1861445327729214 120 16 -1.2937173399007378 120 17 -0.7914804279223588 120 18 -1.6942533628356742 120 19 -0.797619100606866 120 20 0.4891512971083287 120 21 -0.49829799043273226 120 22 -0.19087853241304487 120 23 0.5785408535589411 120 24 0.5742119372586743 120 25 -1.5286083745133396 120 26 -0.6614331702200218 120 27 0.6280973984669487 120 28 0.10011724502835051 120 29 1.2236472563661493 120 30 -1.1417339348319473 120 31 0.7574753040529743 120 32 0.7110066829404111 120 33 0.8779824492438415 120 34 0.16429139726724173 120 35 1.067031142765682 120 36 2.0833006263984544 120 37 2.1630477581840637 120 38 2.871858395217 120 39 1.149915831137136 120 40 1.7293542818381624 120 41 2.57060414208107 120 42 2.7097406194270803 120 43 3.2282530101084994 120 44 2.508259120933559 120 45 2.6472315091529133 120 46 3.643846142274782 120 47 3.6313723333382733 120 48 3.954285449714174 120 49 2.820295861477252 120 50 3.608913606056305 120 51 3.32004356173627 120 52 2.1802233231967136 120 53 1.86452320848678 120 54 2.303655164461155 120 55 2.3463352910062305 120 56 2.9225406203905724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 13.0 5 7.0 6 8.0 7 18.0 8 32.0 9 28.0 10 32.0 11 59.0 12 77.0 13 106.0 14 175.0 15 196.0 16 276.0 17 344.0 18 391.0 19 435.0 20 558.0 21 674.0 22 618.0 23 684.0 24 993.0 25 908.0 26 887.0 27 979.0 28 1029.0 29 1250.0 30 1395.0 31 1697.0 32 1892.0 33 2553.0 34 3315.0 35 4162.0 36 5039.0 37 5093.0 38 2514.0 39 503.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.4196199280945 0.5315870570107858 0.010272213662044172 0.038520801232665644 8 99.26540466956052 0.6036010582282383 0.06934990881771248 0.06164436339352221 9 98.85721623009759 0.598356445814073 0.1617873651771957 0.38263995891114533 10 59.1628145865434 27.46276322547509 5.826913199794555 7.547508988186954 11 33.52850539291217 31.445814072932716 18.310220852593734 16.715459681561377 12 34.368258859784284 17.062146892655367 24.61222393425783 23.95737031330252 13 24.04211607601438 19.48125321006677 26.766820749871595 29.709809964047253 14 22.591165896250644 18.61838726245506 30.431432973805855 28.359013867488443 15 26.69491525423729 17.596302003081664 27.041602465331277 28.667180277349768 16 31.224961479198765 18.906009244992294 24.743194658448896 25.12583461736004 17 29.966615305598353 19.596815613764768 22.796610169491522 27.639958911145353 18 28.751926040061633 21.620441705187467 24.6969696969697 24.930662557781204 19 30.529019003595277 18.972778633795585 22.614278376990242 27.883923985618903 20 27.252182845403183 22.593733949666152 25.187467899332304 24.966615305598356 21 28.651771956856702 18.454031843862353 25.86543400102722 27.028762198253727 22 31.856702619414484 17.8608115048793 24.193631227529533 26.088854648176685 23 29.45814072932717 20.482794042116076 24.35798664612224 25.701078582434516 24 25.738353279572657 24.44912424880579 23.927782628794496 25.88473984282706 25 28.705344530908906 22.06127847548604 21.650358271053243 27.583018722551817 26 27.187010251008402 20.198854147933098 22.215656552680933 30.398479048377563 27 27.457026131195562 18.731210976643798 26.15945939001516 27.652303502145482 28 29.98098077516192 21.859257736198213 21.239847846201297 26.919913642438576 29 28.321588395658658 20.999434185484287 23.339848773211255 27.3391286456458 30 26.987598415067154 19.098955385169557 28.18144393557351 25.73200226418978 31 27.658807783383093 21.4454854318954 23.955008751158243 26.940698033563265 32 30.34569744394965 19.691626554094057 24.021210327164148 25.94146567479214 33 32.14552382914081 20.832153248023893 22.03712762944463 24.985195293390664 34 26.708906402925876 23.36578581363004 25.864111677741718 24.061196105702365 35 24.5781270127528 24.75846966378977 29.23869638026536 21.424706943192064 36 27.44774619210845 23.280327826602406 23.087033839334037 26.184892141955103 37 26.763431989275034 22.847272352273897 25.36351448901722 25.02578116943385 38 30.361010830324908 21.580711707065497 26.214543579164516 21.843733883445076 39 29.927496968289603 21.358722295327297 22.414015532677965 26.29976520370514 40 26.558545529673054 21.406435617994937 24.701719952481792 27.333298899850217 41 25.63215125138828 22.55340031510706 25.92917839708655 25.885270036418113 42 25.534717916924986 19.717400289315975 25.932527381690434 28.81535441206861 43 24.0348338415422 20.572122590314745 28.425241614553727 26.967801953589333 44 28.974908912374996 19.873898550350138 25.396005064730353 25.75518747254451 45 25.12666735601282 20.507186433667666 25.560955433771067 28.805190776548447 46 28.705906692872656 19.993276093927793 24.806041170994103 26.49477604220544 47 27.936992395633958 22.313899953442657 25.24442605142 24.50468159950339 48 24.103307282231768 23.489984990424926 24.820143884892087 27.586563842451216 49 26.592496765847347 23.1875808538163 24.716688227684347 25.503234152652006 50 25.12685098891995 25.742984363674022 24.381277829553692 24.748886817852338 51 25.352622997489583 27.839747405471154 23.760966898729265 23.046662698309998 52 28.29817522971399 22.64268150640611 26.12139251973599 22.937750744143912 53 29.621288602417746 22.880588128704925 24.935932282363904 22.56219098651342 54 26.03061197882715 27.03449714479414 24.012411671142864 22.92247920523585 55 31.896931244335104 22.294445163796453 22.29185549656869 23.516768095299753 56 27.872290841295733 24.21347005360056 22.706439835314224 25.207799269789483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.5 9 5.0 10 3.0 11 1.0 12 15.5 13 30.5 14 31.0 15 29.5 16 28.0 17 36.0 18 44.0 19 61.0 20 78.0 21 90.0 22 123.5 23 145.0 24 163.5 25 182.0 26 239.0 27 296.0 28 362.5 29 419.0 30 409.0 31 438.5 32 468.0 33 549.5 34 631.0 35 553.5 36 476.0 37 510.5 38 543.5 39 542.0 40 594.5 41 647.0 42 698.5 43 750.0 44 841.5 45 933.0 46 962.5 47 1265.0 48 1538.0 49 1360.5 50 1183.0 51 1158.5 52 1134.0 53 1263.5 54 1477.5 55 1562.0 56 1604.5 57 1647.0 58 1890.0 59 2133.0 60 2432.5 61 2732.0 62 2873.5 63 3119.0 64 3223.0 65 2838.5 66 2454.0 67 2233.0 68 2012.0 69 1826.5 70 1641.0 71 2012.0 72 1738.5 73 1094.0 74 906.5 75 719.0 76 624.0 77 529.0 78 427.0 79 265.0 80 205.0 81 167.5 82 130.0 83 94.0 84 58.0 85 49.0 86 40.0 87 27.0 88 11.0 89 8.0 90 5.5 91 3.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0179763739085773 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005136106831022086 25 0.007704160246533128 26 0.04365690806368772 27 0.053929121725731895 28 0.08217770929635337 29 0.14894709809964046 30 0.19003595274781715 31 0.22598870056497175 32 0.23369286081150487 33 0.2593733949666153 34 0.29275808936825887 35 0.3210066769388803 36 0.3569594247560349 37 0.39034411915767847 38 0.4108885464817668 39 0.4699537750385208 40 0.5598356445814073 41 0.5726759116589625 42 0.5855161787365177 43 0.6214689265536724 44 0.6189008731381612 45 0.657421674370827 46 0.6985105290190036 47 0.7139188495120699 48 0.7652799178222907 49 0.7447354904982024 50 0.8012326656394453 51 0.7729840780688237 52 0.783256291730868 53 0.7935285053929122 54 1.512583461736004 55 0.8346173600410888 56 0.8243451463790448 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 38940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.365690806368775 #Duplication Level Percentage of deduplicated Percentage of total 1 70.16670525584627 31.129943502824858 2 12.792313035424868 11.350796096558808 3 5.643667515628618 7.5115562403698 4 3.4209307710118084 6.070878274268105 5 1.9448946515397085 4.314329738058552 6 1.3776337115072934 3.6671802773497686 7 0.9087751794396852 2.822290703646636 8 0.654086594119009 2.3215202876219827 9 0.5209539245195647 2.0801232665639446 >10 2.4195415605464228 19.48125321006677 >50 0.09261403102570039 2.868515665125835 >100 0.04630701551285019 2.9301489470981 >500 0.011576753878212548 3.4514637904468413 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 834 2.14175654853621 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 510 1.3097072419106317 No Hit GTCGGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG 315 0.8089368258859784 No Hit GTCGGTGGGGGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTGA 130 0.3338469440164355 No Hit GTCGGTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC 127 0.3261427837699024 No Hit GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 123 0.31587057010785824 No Hit GTCGGTGGGAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCCT 117 0.30046224961479195 No Hit GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 115 0.2953261427837699 No Hit GTCGGTGGGGAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT 110 0.2824858757062147 No Hit GTCGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.26707755521314847 No Hit GTCGGTGGGGCACCCGCTTCACCGACGACTACCAGCTTTTCGAGGAGCTCGGCAAG 99 0.2542372881355932 No Hit GTCGGTGGGGGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTTTG 95 0.24396507447354907 No Hit GTCGGTGGGAGAGTCACTCACTCCATCACCGGGAAATATGCAAGCATAGTTTACAA 94 0.241397021058038 No Hit GTCGGTGGGAGACTCAGCACCTTGCCCATGCCAGGCATTGCACTACTGCTGAGCTA 91 0.23369286081150487 No Hit GTCGGTGGGGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGT 76 0.19517205957883924 No Hit GTCGGTGGGAACCTTCTCTAACCCACGATATCAAGACCCAGAAACTGAAGAAAAAT 72 0.18489984591679506 No Hit GTCGGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 68 0.1746276322547509 No Hit GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 67 0.17205957883923986 No Hit GTCGGTGGGATTCCTGGAGTTCCTGTCCAGCCCGTGGTGCTACGCTACCCAAACAA 66 0.1694915254237288 No Hit GTCGGTGGGGGCAGGCTCTGTGCGCCTACCAAGCAGAGCCAAACACCTGTGCCGCC 66 0.1694915254237288 No Hit GTCGGTGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCT 60 0.15408320493066258 No Hit GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 55 0.14124293785310735 No Hit GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGGGA 55 0.14124293785310735 No Hit GTCGGTGGGATCGGTTGGCTGCAAAGTCCTTGATTCAGACTAAGGATTTTGGATCC 51 0.13097072419106318 No Hit GTCGGTGGGCTCTAGTTTGTTGTTGATGGGTTGTTTTCTGAAACAGGGTCTCATCA 51 0.13097072419106318 No Hit GTCGGTGGGACCCACTGCTGGTGGTTGTTTCCTGCCTTACCCGGGACCCCTTCAGC 51 0.13097072419106318 No Hit GTCGGTGGGATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGT 50 0.12840267077555212 No Hit GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 50 0.12840267077555212 No Hit GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 49 0.1258346173600411 No Hit GTCGGTGGGGCCTGGGAAGAACGTGCAGCTGACAGAGAACGAGATCCGTGGTCTGT 49 0.1258346173600411 No Hit GTCGGTGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT 48 0.12326656394453005 No Hit GTCGGTGGGGATGCTCTGTCCTCTCCACACAGGCCTCTTGCTCCTAACAGCTCAGC 45 0.11556240369799693 No Hit GTCGGTGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG 45 0.11556240369799693 No Hit GTCGGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 45 0.11556240369799693 No Hit GTCGGTGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGC 45 0.11556240369799693 No Hit GTCGGTGGGGTCTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC 44 0.11299435028248588 No Hit GTCGGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA 43 0.11042629686697483 No Hit GTCGGTGGGGAGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA 43 0.11042629686697483 No Hit GTCGGTGGGGAACTACCAGCCTCCCACTGTGGTACCCGGTGGTGACCTGGCCAAGG 42 0.10785824345146379 No Hit GTCGGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGG 42 0.10785824345146379 No Hit GTCGGTGGGGAGCCCTTTCTCCTCTACTCGCAAAGATGACGCTTTGCCGTCCTGGC 41 0.10529019003595276 No Hit GTCGGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 40 0.1027221366204417 No Hit GTCGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 39 0.10015408320493066 No Hit GTCGGTGGGATGAAGGAAATCCTAAGGTTCAATGTTCAAGAGAACGGGTTTTTATC 39 0.10015408320493066 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0025680534155110425 0.0 0.0 0.0 0.0 10 0.005136106831022085 0.0 0.0 0.0 0.0 11 0.01027221366204417 0.0 0.0 0.0 0.0 12 0.01027221366204417 0.0 0.0 0.0 0.0 13 0.01027221366204417 0.0 0.0 0.0 0.0 14 0.015408320493066256 0.0 0.0 0.0 0.0 15 0.015408320493066256 0.0 0.0 0.0 0.0 16 0.015408320493066256 0.0 0.0 0.0 0.0 17 0.015408320493066256 0.0 0.0 0.0 0.0 18 0.015408320493066256 0.0 0.0 0.0 0.0 19 0.015408320493066256 0.0 0.0 0.0 0.0 20 0.02054442732408834 0.0 0.0 0.0 0.0 21 0.02054442732408834 0.0 0.0 0.0 0.0 22 0.02824858757062147 0.0 0.0 0.0 0.0 23 0.030816640986132512 0.0 0.0 0.0 0.0 24 0.030816640986132512 0.0 0.0 0.0 0.0 25 0.030816640986132512 0.0 0.0 0.0 0.0 26 0.035952747817154594 0.0 0.0 0.0 0.0 27 0.035952747817154594 0.0 0.0 0.0 0.0 28 0.03852080123266564 0.0 0.0 0.0 0.0 29 0.03852080123266564 0.0 0.0 0.0 0.0 30 0.04365690806368772 0.0 0.0 0.0 0.0 31 0.053929121725731895 0.0 0.0 0.0 0.0 32 0.05906522855675398 0.0 0.0 0.0 0.0 33 0.05906522855675398 0.0 0.0 0.0 0.0 34 0.061633281972265024 0.0 0.0 0.0 0.0 35 0.06933744221879815 0.0 0.0 0.0 0.0 36 0.07190549563430919 0.0 0.0 0.0 0.0 37 0.07447354904982023 0.0 0.0 0.0 0.0 38 0.07447354904982023 0.0 0.0 0.0 0.0 39 0.07704160246533127 0.0 0.0 0.0 0.0 40 0.0847457627118644 0.0 0.0 0.0 0.0 41 0.08731381612737545 0.0 0.0 0.0 0.0 42 0.09244992295839753 0.0 0.0 0.0 0.0 43 0.09501797637390857 0.0 0.0 0.0 0.0 44 0.10015408320493066 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGTGA 15 0.008025708 50.53325 50 TTCCTCA 15 0.008025708 50.53325 50 TCTTGTG 15 0.008025708 50.53325 49 CTTGTGC 15 0.008025708 50.53325 50 GCATATT 15 0.008025708 50.53325 49 GCCCTGT 15 0.008025708 50.53325 48 TGCGCCC 15 0.008234912 50.20596 42 TATAAGC 15 0.008234912 50.20596 34 ACGAGAT 15 0.008234912 50.20596 39 TCAGACT 25 2.0006148E-5 50.20596 35 GAATCTC 15 0.008234912 50.20596 39 AGAGAAC 15 0.008234912 50.20596 34 ATATGCT 100 0.0 50.20596 34 CAGACTA 15 0.008234912 50.20596 36 GCCGCAT 15 0.008234912 50.20596 40 GCGTAGA 30 9.929936E-7 50.20596 38 CAGCGTA 30 9.929936E-7 50.20596 36 AAGCCTG 15 0.008234912 50.20596 37 TTCAGAC 20 4.050458E-4 50.20596 34 GCCTGCG 15 0.008234912 50.20596 39 >>END_MODULE