FastQCFastQC Report
Fri 17 Jun 2016
SRR1526902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17775
Sequences flagged as poor quality0
Sequence length64
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGTATACATTTATAT1290.7257383966244726No Hit
CTGTGTGGGGAACAAAATAATCCCATTAATATCTTTCTTGGATTCTCAGCAGTTTTTGTTTTTT700.39381153305203936No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.37693389592123766No Hit
CTGTGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA660.37130801687763715No Hit
CTGTGTATTTCTATCATACGCCACGTTTACTTTGGTCCTGTATATGTATTTAAATGTTTGAAGT630.35443037974683544No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.32067510548523204No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.3037974683544304No Hit
CTGTGTGGGATGGTCATGTATTCAATCACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGT520.29254571026722925No Hit
CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT450.25316455696202533No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTGTTTGTTTCTCAAAA350.19690576652601968No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.19690576652601968No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.17440225035161744No Hit
CTGTGTGGGATCCTTTCTTGTTATTCCTCTTCCCTCACCCCTACCTACTCCATTCTCATGGAGT290.1631504922644163No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTGTTTGTTTCTCTTAA290.1631504922644163No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAACTGTGTTTGTTTCTCTTAA210.1181434599156118No Hit
CTGTGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.1181434599156118No Hit
CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC210.1181434599156118No Hit
CTGTGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.11251758087201125No Hit
CTGTGTGGGGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTA200.11251758087201125No Hit
CTGTGTGGGACTCTTGTCAGTTATTAAACAAACAAACAGAAAAAAATTGTTTTGAAAAACCTTG190.10689170182841068No Hit
CTGTGTGGGGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAG190.10689170182841068No Hit
CTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10689170182841068No Hit
CTGTGTGGGGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTG180.10126582278481014No Hit
CTGTGTGGGACTGAAAGCTTCATTTAAAGACAAGAACTGTCCAGTTGGGACTCTGTCTCCCCCA180.10126582278481014No Hit
CTGTGTGGGGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACATAA180.10126582278481014No Hit
CTGTGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG180.10126582278481014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTA150.004403533458.6771457
TTGTTTC150.004555537458.1784752
GTTTGTT201.9268991E-458.1784750
GTTTGAT150.004555537458.1784748
TATACAT150.004555537458.1784752
TTTCTCT150.004555537458.1784755
GCTGGCT201.9268991E-458.1784753
CCCATTT150.004555537458.1784747
TACATTT150.004555537458.1784754
ACATTTA201.9268991E-458.1784755
GAGTTTG150.004555537458.1784746
GTATACA150.004555537458.1784751
CGTGGCC150.004555537458.1784745
GCTGCTT150.004555537458.1784742
CCATTTG150.004555537458.1784748
TGCTTAA150.004555537458.1784744
TGGATGT201.9268991E-458.1784753
TAGAGTT150.004555537458.1784744
CTTAACA150.004555537458.1784746
TTTGGAT201.9268991E-458.1784751
Produced by FastQC (version 0.11.5)