FastQCFastQC Report
Fri 17 Jun 2016
SRR1526901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20253
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGTATACATTTATAT1770.8739446007998815No Hit
CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1080.5332543326914532No Hit
CTGTGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA990.4888164716338321No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.4443786105762109No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.41475336987113015No Hit
CTGTGTGGGGAACAAAATAATCCCATTAATATCTTTCTTGGATTCTCAGCAGTTTTTGTTTTTT820.4048782896361033No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT790.39006566928356295No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.36537796869599565No Hit
CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT700.3456278082259418No Hit
CTGTGTGGGATCCTTTCTTGTTATTCCTCTTCCCTCACCCCTACCTACTCCATTCTCATGGAGT610.30118994716832076No Hit
CTGTGTGGGATGGAGAACCCTGACATGGGGGCAGGAACCCCCATACCCTGCCACCACAGGGCCT520.25675208611069966No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC450.22218930528810546No Hit
CTGTGTGGGGTTGGCCAGACTTAACTCTGCTCCCTTGACCACAGAGATGAGATTCTGAGCTGCC410.20243914481805164No Hit
CTGTGTGGGGGAAGAACGTCTGCTCAGATGGACCCTGAGATGTCTCCACGGACTTTATTTGGGA400.19750160470053818No Hit
CTGTGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG390.19256406458302472No Hit
CTGTGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG390.19256406458302472No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.19256406458302472No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.16787636399545747No Hit
CTGTGTGGGATTGGGGGAGGAGATGACTCCTTCAACACCTTCTTCAGTGAGACAGGAGCTGGCA330.162938823877944No Hit
CTGTGTGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGCGGACCGCGCCGG300.14812620352540362No Hit
CTGTGTGGGGTCTCCATTGGTAGCGTAACGAGCTCCTTCCATAGCCTTGGAAATAGTCTGGGTT300.14812620352540362No Hit
CTGTGTGGGGGCCTTAGACTGGAATTGGCTGTCTCGGGGCTCTGGCCGCCGGCCTCTCCTCCGC290.1431886634078902No Hit
CTGTGTGGGGTGTGGGCAGGGGTGTGGGGGATAACCTGTGAGTGCAGCCGGGTTGGATGCCGTG290.1431886634078902No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.13825112329037673No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.12837604305534983No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.12343850293783637No Hit
CTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.12343850293783637No Hit
CTGTGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG250.12343850293783637No Hit
CTGTGTGGGAACAACAACAACCACAACCATAACCACAACCACCACCGAAGAGGCCAGGCTCGCC250.12343850293783637No Hit
CTGTGTGGGGAAACTTTTTTTTCAGTTCTTGAGACAGGGTCTCACTATGTCACCTTGGCTGATC250.12343850293783637No Hit
CTGTGTGGGCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC240.11850096282032291No Hit
CTGTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA240.11850096282032291No Hit
CTGTGTGGGAAGCAAAAGCGATTGCCTTAGTAGCGGCGAGCGAAACGGCAGGGGGGGAAACCGA230.11356342270280946No Hit
CTGTGTGGGACTGAAAGCTTCATTTAAAGACAAGAACTGTCCAGTTGGGACTCTGTCTCCCCCA230.11356342270280946No Hit
CTGTGTGGGGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACATAA230.11356342270280946No Hit
CTGTGTGGGGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTA230.11356342270280946No Hit
CTGTGTGGGCTAAGGGGAGAGGCTCAGAGGAAAATGGCAAAGCCTTCCTCTGGACCAAATGTAG230.11356342270280946No Hit
CTGTGTGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCACGCAAGGGG220.10862588258529601No Hit
CTGTGTGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG220.10862588258529601No Hit
CTGTGTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGAG220.10862588258529601No Hit
CTGTGTGGGATCTCTGCCCAGGACTCTCAGTGGGAGCTCCAGCTGACGAGGTAAGGGGGTTAAT220.10862588258529601No Hit
CTGTGTGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA210.10368834246778254No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.10368834246778254No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGACACTCCGCCGCCGGCTTACACTGCGCTTCTTG210.10368834246778254No Hit
CTGTGTGGGGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTG210.10368834246778254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTC150.004525759358.30423755
GTCGTGA150.004525759358.30423757
GGCGCCA150.004525759358.30423749
CCACGCA150.004525759358.30423753
TAAAAGG150.004525759358.30423751
CGCCACG150.004525759358.30423751
TCGTGAA150.004525759358.30423758
AAAAGGT150.004525759358.30423752
AGGTCGT150.004525759358.30423755
GCCACGC150.004525759358.30423752
CAAGGGG150.004525759358.30423758
CCGGATT150.00457077658.15920345
CGCCTTC150.00457077658.15920338
CTCCTCT150.00457077658.15920354
CCCGGAT150.00457077658.15920344
GGCAAAT150.00457077658.15920336
AAATACC150.00457077658.15920339
ACCCGGA150.00457077658.15920343
ATCAGAA150.00457077658.15920340
GCAAATA150.00457077658.15920337
Produced by FastQC (version 0.11.5)