FastQCFastQC Report
Fri 17 Jun 2016
SRR1526900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20523
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGTATACATTTATAT1810.881937338595722No Hit
CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC940.45802270623203234No Hit
CTGTGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA870.42391463236368954No Hit
CTGTGTGGGATCCTTTCTTGTTATTCCTCTTCCCTCACCCCTACCTACTCCATTCTCATGGAGT870.42391463236368954No Hit
CTGTGTGGGGAACAAAATAATCCCATTAATATCTTTCTTGGATTCTCAGCAGTTTTTGTTTTTT840.409296886420114No Hit
CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT820.39955172245773035No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.3898065584953467No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.3654436485893875No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT580.2826097549091263No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.2582468450031672No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC510.24850168104078352No Hit
CTGTGTGGGGGAAGAACGTCTGCTCAGATGGACCCTGAGATGTCTCCACGGACTTTATTTGGGA420.204648443210057No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.1900306972664815No Hit
CTGTGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG380.18515811528528967No Hit
CTGTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA360.17541295132290602No Hit
CTGTGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG350.17054036934171418No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.17054036934171418No Hit
CTGTGTGGGATGGAGAACCCTGACATGGGGGCAGGAACCCCCATACCCTGCCACCACAGGGCCT340.16566778736052234No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.16566778736052234No Hit
CTGTGTGGGCTAAGGGGAGAGGCTCAGAGGAAAATGGCAAAGCCTTCCTCTGGACCAAATGTAG330.1607952053793305No Hit
CTGTGTGGGGTTGGCCAGACTTAACTCTGCTCCCTTGACCACAGAGATGAGATTCTGAGCTGCC320.15592262339813867No Hit
CTGTGTGGGGGCCTTAGACTGGAATTGGCTGTCTCGGGGCTCTGGCCGCCGGCCTCTCCTCCGC310.15105004141694683No Hit
CTGTGTGGGGTGTGGGCAGGGGTGTGGGGGATAACCTGTGAGTGCAGCCGGGTTGGATGCCGTG300.146177459435755No Hit
CTGTGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.14130487745456316No Hit
CTGTGTGGGATTGGGGGAGGAGATGACTCCTTCAACACCTTCTTCAGTGAGACAGGAGCTGGCA290.14130487745456316No Hit
CTGTGTGGGACTGAAAGCTTCATTTAAAGACAAGAACTGTCCAGTTGGGACTCTGTCTCCCCCA280.13643229547337132No Hit
CTGTGTGGGGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTA270.13155971349217951No Hit
CTGTGTGGGGTCTCCATTGGTAGCGTAACGAGCTCCTTCCATAGCCTTGGAAATAGTCTGGGTT270.13155971349217951No Hit
CTGTGTGGGAGACTGACCCGGGCAGCCAAGGTGTTGGAGCAGCTCACAGGCCAGACCCCGGTGT250.12181454952979584No Hit
CTGTGTGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGCGGACCGCGCCGG250.12181454952979584No Hit
CTGTGTGGGGAAACTTTTTTTTCAGTTCTTGAGACAGGGTCTCACTATGTCACCTTGGCTGATC250.12181454952979584No Hit
CTGTGTGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG240.116941967548604No Hit
CTGTGTGGGGATCGGAGCGTCTTCCGCAGCCCGGCCTTCACCATGAGCGTCCCGGCCTTCATCG240.116941967548604No Hit
CTGTGTGGGATCTCTGCCCAGGACTCTCAGTGGGAGCTCCAGCTGACGAGGTAAGGGGGTTAAT240.116941967548604No Hit
CTGTGTTTGAAGCTTGCCGGTAGTGTTGCTTTGAGTTACAGTATAGCCTGCGTTTATCCTCTGT240.116941967548604No Hit
CTGTGTGGGGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTG240.116941967548604No Hit
CTGTGTGGGAAGCAAAAGCGATTGCCTTAGTAGCGGCGAGCGAAACGGCAGGGGGGGAAACCGA220.10719680358622033No Hit
CTGTGTGGGAACTTCCACACGGAGCAGATGTACAAACACAAGCTGGTGGACTTTGTAATCCACT210.1023242216050285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCT150.004435287358.6039644
CTTGCTT150.004435287358.6039645
ACGGCAG150.004435287358.6039645
AACGGCA150.004435287358.6039644
GAGGTCC201.9115684E-458.3152725
CTTAGTA201.9115684E-458.3152726
TGGAGGT201.9115684E-458.3152723
GATTGCC201.9115684E-458.3152720
ATTGCCT201.9115684E-458.3152721
GGAGGTC201.9115684E-458.3152724
CCTTAGT201.9115684E-458.3152725
TTGGAGG201.9115684E-458.3152722
GTGTCTT150.004523186558.31526641
GAAACGG150.004523186558.31526642
TGTCTTG150.004523186558.31526642
GCGAAAC150.004523186558.31526640
GTCTTGC150.004523186558.31526643
CGAAACG150.004523186558.31526641
AAACGGC150.004523186558.31526643
AGCGAAA150.004523186558.31526639
Produced by FastQC (version 0.11.5)