FastQCFastQC Report
Fri 17 Jun 2016
SRR1526899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17842
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGTATAC1780.997646003811232No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1010.5660800358704181No Hit
CTGTGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT940.5268467660576168No Hit
CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG930.5212420132272166No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA880.4932182490752158No Hit
CTGTGTGGGATCCTTTCTTGTTATTCCTCTTCCCTCACCCCTACCTACTCCATTCT830.4651944849232149No Hit
CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC810.45398497926241455No Hit
CTGTGTGGGAAGCAAAAGCGATTGCCTTAGTAGCGGCGAGCGAAACGGCAGGGGGG710.39793745095841276No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC700.3923326981280125No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.3250756641632104No Hit
CTGTGTGGGGAACAAAATAATCCCATTAATATCTTTCTTGGATTCTCAGCAGTTTT550.3082614056720099No Hit
CTGTGTGGGATGGAGAACCCTGACATGGGGGCAGGAACCCCCATACCCTGCCACCA490.2746328886896088No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA400.22419011321600715No Hit
CTGTGTGGGGTTGGCCAGACTTAACTCTGCTCCCTTGACCACAGAGATGAGATTCT390.218585360385607No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTGTTTGTT380.2129806075552068No Hit
CTGTGTGGGGGAAGAACGTCTGCTCAGATGGACCCTGAGATGTCTCCACGGACTTT360.20177110189440645No Hit
CTGTGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA350.19616634906400626No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA340.19056159623360608No Hit
CTGTGTGGGGTCTCCATTGGTAGCGTAACGAGCTCCTTCCATAGCCTTGGAAATAG330.18495684340320592No Hit
CTGTGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG320.17935209057280574No Hit
CTGTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA320.17935209057280574No Hit
CTGTGTGGGATTGGGGGAGGAGATGACTCCTTCAACACCTTCTTCAGTGAGACAGG310.17374733774240556No Hit
CTGTGTGGGGAAACTTTTTTTTCAGTTCTTGAGACAGGGTCTCACTATGTCACCTT310.17374733774240556No Hit
CTGTGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG300.16814258491200537No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.15693307925120503No Hit
CTGTGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.15693307925120503No Hit
CTGTGTGGGGTTAAAAGGAACAGAATACAAAGTCTCTGTAATTCATAGAGCTAAAT280.15693307925120503No Hit
CTGTGTGGGCTAAGGGGAGAGGCTCAGAGGAAAATGGCAAAGCCTTCCTCTGGACC260.14572357359040466No Hit
CTGTGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC250.14011882076000448No Hit
CTGTGTGGGGTGTGGGCAGGGGTGTGGGGGATAACCTGTGAGTGCAGCCGGGTTGG250.14011882076000448No Hit
CTGTGTGGGGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGG230.1289093150992041No Hit
CTGTGTGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGCGGAC220.12330456226880394No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.11769980943840377No Hit
CTGTGTGGGGGTGGGCGGAACACATCGTCTCTCCGCCATGGCGGCCCTGCGTGCTC210.11769980943840377No Hit
CTGTGTGGGCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC210.11769980943840377No Hit
CTGTGTGGGGGCCTTAGACTGGAATTGGCTGTCTCGGGGCTCTGGCCGCCGGCCTC210.11769980943840377No Hit
CTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.11209505660800358No Hit
CTGTGTGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC200.11209505660800358No Hit
CTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.1064903037776034No Hit
CTGTGTTTGAAGCTTGCCGGTAGTGTTGCTTTGAGTTACAGTATAGCCTGCGTTTA190.1064903037776034No Hit
CTGTGTGGGATCTCTGCCCAGGACTCTCAGTGGGAGCTCCAGCTGACGAGGTAAGG190.1064903037776034No Hit
CTGTGTGGGCGGGTCGTGCCTTTGCATCGCTGAAGGAACTTTTTGTCTTTTAAGGA190.1064903037776034No Hit
CTGTGTGGGGGTCTGAGTCCTGGCCGAGGAACTTCTGTTGGTGTTTGGGGCCGCAG190.1064903037776034No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10088555094720322No Hit
CTGTGTGGGACTCTTGTCAGTTATTAAACAAACAAACAGAAAAAAATTGTTTTGAA180.10088555094720322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTCC150.00812360750.26420634
AAACTCT150.00812360750.26420634
TAGCCTT150.00812360750.26420642
CGCCATG150.00812360750.26420634
GCGGCCC150.00812360750.26420641
ACGTACT150.00812360750.26420641
GAGCTCC150.00812360750.26420630
ACTCTAC150.00812360750.26420636
TCTACGT203.9672165E-450.26420638
CTCTACG150.00812360750.26420637
AACTCTA150.00812360750.26420635
CTTGGAA150.00812360750.26420646
TACGTAC150.00812360750.26420640
TTCCATA150.00812360750.26420637
CGTACTG150.00812360750.26420642
CCTTCCA150.00812360750.26420635
CTACGTA150.00812360750.26420639
CATAGCC150.00812360750.26420640
TCTCCGC150.00812360750.26420630
CATGGCG150.00812360750.26420637