##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526899_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17842 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.51221836117027 38.0 38.0 40.0 33.0 40.0 2 37.04691178119045 38.0 37.0 40.0 33.0 40.0 3 36.757650487613496 38.0 37.0 39.0 32.0 40.0 4 35.88818518103351 38.0 35.0 39.0 29.0 40.0 5 36.73203676717857 38.0 37.0 40.0 32.0 40.0 6 36.70737585472481 38.0 36.0 40.0 31.0 40.0 7 37.66405111534581 38.0 38.0 40.0 34.0 40.0 8 37.627956507118036 38.0 38.0 40.0 34.0 40.0 9 37.62268803945746 38.0 38.0 40.0 34.0 40.0 10 36.2366887120278 38.0 35.0 40.0 30.0 40.0 11 36.517598923887455 39.0 35.0 40.0 31.0 40.0 12 36.86245936554198 39.0 35.0 40.0 31.0 40.0 13 36.478757986772784 39.0 35.0 40.0 31.0 40.0 14 37.14936666293016 39.0 37.0 40.0 33.0 40.0 15 37.17901580540298 39.0 37.0 40.0 33.0 40.0 16 37.20053805627172 39.0 37.0 40.0 33.0 40.0 17 37.200930388969844 39.0 37.0 40.0 33.0 40.0 18 36.96822105145163 39.0 36.0 40.0 32.0 40.0 19 37.13434592534469 39.0 37.0 40.0 33.0 40.0 20 36.956843403205916 39.0 36.0 40.0 32.0 40.0 21 36.93924447931846 39.0 36.0 40.0 32.0 40.0 22 36.799966371483016 39.0 36.0 40.0 32.0 40.0 23 36.6442663378545 39.0 36.0 40.0 31.0 40.0 24 36.54349288196391 38.0 36.0 40.0 31.0 40.0 25 36.16797444232709 38.0 35.0 40.0 30.0 40.0 26 35.91917946418563 38.0 35.0 40.0 30.0 40.0 27 35.536935321152335 38.0 35.0 40.0 29.0 40.0 28 35.694316780629975 38.0 35.0 40.0 29.0 40.0 29 35.51569330792512 38.0 35.0 40.0 29.0 40.0 30 35.22564734895191 38.0 35.0 40.0 28.0 40.0 31 34.59270261181482 38.0 34.0 39.0 26.0 40.0 32 34.27211074991593 38.0 33.0 39.0 25.0 40.0 33 34.31767739042708 38.0 33.0 39.0 25.0 40.0 34 34.48430669207488 38.0 34.0 40.0 26.0 40.0 35 34.30753278780406 38.0 33.0 40.0 25.0 40.0 36 34.095897320928145 38.0 34.0 40.0 23.0 40.0 37 33.84788700818294 38.0 33.0 40.0 21.0 40.0 38 33.70597466651721 38.0 33.0 39.0 21.0 40.0 39 33.55033068041699 38.0 33.0 39.0 19.0 40.0 40 33.34979262414527 38.0 33.0 39.0 17.0 40.0 41 33.00947203228338 38.0 33.0 39.0 10.0 40.0 42 32.74453536599036 37.0 32.0 39.0 7.0 40.0 43 32.63496244815604 37.0 32.0 39.0 4.0 40.0 44 32.46872547920637 37.0 32.0 39.0 4.0 40.0 45 32.22065911893286 37.0 32.0 39.0 4.0 40.0 46 31.6989126779509 36.0 31.0 39.0 4.0 40.0 47 31.523203676717856 36.0 31.0 39.0 4.0 40.0 48 31.136027351193814 36.0 30.0 39.0 4.0 40.0 49 31.00952807981168 36.0 30.0 39.0 4.0 40.0 50 30.53727160632216 36.0 30.0 39.0 4.0 40.0 51 29.921253222732876 36.0 29.0 39.0 4.0 40.0 52 29.575944400851924 35.0 29.0 39.0 4.0 40.0 53 29.31958300638942 35.0 28.0 39.0 4.0 40.0 54 28.7563053469342 35.0 26.0 39.0 4.0 40.0 55 28.360609797107948 35.0 23.0 39.0 4.0 40.0 56 16.710738706423047 20.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.13313820249063468 1 2 0.31533209814846686 1 3 -0.08502591836131757 1 4 -0.8324805336916015 1 5 -0.5768190006017448 1 6 -0.9023579341238843 1 7 -0.14069114070968425 1 8 -0.34227625439315545 1 9 -0.07434129760500952 1 10 -1.1661138512720441 1 11 -1.0548167758807807 1 12 -2.2551220186415506 1 13 -1.1738811738873451 1 14 -0.4841780075529485 1 15 -1.3326272062369426 1 16 -0.740778754940898 1 17 -0.3265449090646797 1 18 -0.4810374677774547 1 19 -0.29690763183099733 1 20 -3.330189308000726 1 21 -1.9339695003189803 1 22 -2.052688647102876 1 23 -2.474711189151378 1 24 -1.998878080644019 1 25 -4.734676396953951 1 26 -3.6210264422511145 1 27 -4.069984788787309 1 28 -2.082492718052528 1 29 -2.960263539805048 1 30 -1.1501595887223743 1 31 -2.507342107280323 1 32 -3.177887746350411 1 33 -2.054333369848699 1 34 -3.7295920253470918 1 35 -3.2309017498402106 1 36 -3.3521856178368807 1 37 -4.10428631482759 1 38 -4.1238084977580804 1 39 -3.153844033842798 1 40 -8.135055462607372 1 41 -4.197737850796429 1 42 -3.9421733625577 1 43 -4.453959215662142 1 44 -5.852640513218859 1 45 -3.7452781489669817 1 46 -8.445270438492152 1 47 -7.358770001213156 1 48 -6.211658192688834 1 49 -6.617709021768594 1 50 -6.3792955643796 1 51 -7.6293755662144065 1 52 -7.57316889048229 1 53 -7.029417030764037 1 54 -5.913513293703613 1 55 -8.191859843484913 1 56 -4.7623927927128555 2 1 0.5775826469350847 2 2 0.19310987592624684 2 3 1.2371963038609053 2 4 1.256408355197287 2 5 0.8342921105093666 2 6 0.6754198436538914 2 7 0.59264219262365 2 8 0.5466126344957303 2 9 0.43676981350610333 2 10 0.21166392650572874 2 11 0.36740544634143646 2 12 -1.355122018641552 2 13 -0.7738811738873466 2 14 -0.828622451997397 2 15 0.5784839048741688 2 16 0.35922124505910347 2 17 0.9067884242686546 2 18 0.9967403100003196 2 19 0.33642570150233553 2 20 0.7364773586659368 2 21 0.9326971663476868 2 22 1.2473113528971211 2 23 0.8030665886264003 2 24 1.501121919355981 2 25 2.031990269712715 2 26 1.9234180021933298 2 27 1.552237433434911 2 28 1.6286183930585807 2 29 1.4286253490838448 2 30 0.5720626334998471 2 31 2.514880114941896 2 32 2.655445586982921 2 33 3.2123332968179668 2 34 1.6370746413195718 2 35 1.202431583493123 2 36 2.0033699377186736 2 37 1.7512692407279644 2 38 1.7650803911308088 2 39 1.4017115217127554 2 40 2.6093889818370712 2 41 0.3244843714257897 2 42 -0.18661780700214337 2 43 0.40159633989341614 2 44 2.4362483756700257 2 45 -1.0897225934114232 2 46 -0.4786037718254832 2 47 0.8301188876757308 2 48 1.3216751406445013 2 49 0.2378465337869642 2 50 2.1873711022870665 2 51 2.5706244337855964 2 52 3.5712755539621526 2 53 3.2705829692359636 2 54 4.397597817407501 2 55 4.685917934292863 2 56 3.2931627628427016 3 1 -0.08908401973158675 3 2 0.24866543148180398 3 3 -0.04058147391687328 3 4 0.70085279964173 3 5 0.556514332731588 3 6 0.8420865103205628 3 7 0.7037533037347572 3 8 0.7688348567179517 3 9 0.7701031468394319 3 10 -0.23278051793871413 3 11 1.089627668563665 3 12 1.0893224258028908 3 13 1.003896603890432 3 14 1.22693310355816 3 15 0.6340394604297259 3 16 1.5258879117257678 3 17 1.3512328687131046 3 18 1.3300736433336553 3 19 1.4475368126134498 3 20 1.6253662475548296 3 21 0.3771416107921297 3 22 1.2473113528971211 3 23 1.5252888108486218 3 24 0.3900108082448739 3 25 1.4764347141571648 3 26 1.534529113304444 3 27 1.3855707667682466 3 28 0.5730628375030236 3 29 2.4286253490838448 3 30 2.8498404112776257 3 31 3.12599122605301 3 32 2.544334475871814 3 33 2.0456666301513025 3 34 0.1370746413195718 3 35 2.0357649168264587 3 36 1.725592159940895 3 37 0.8068247962835216 3 38 2.0428581689085874 3 39 0.9017115217127554 3 40 2.442722315170407 3 41 1.8800399269813468 3 42 1.8133821929978566 3 43 2.7349296732267447 3 44 0.6584705978922543 3 45 1.9102774065885768 3 46 2.299174005952292 3 47 1.941229998786845 3 48 1.9883418073111656 3 49 2.737846533786964 3 50 3.465148880064845 3 51 1.7928466560078178 3 52 2.5712755539621526 3 53 2.3816940803470743 3 54 2.619820039629719 3 55 1.1859179342928634 3 56 0.515384985064923 4 1 -0.10495703560459901 4 2 -0.08466790185153172 4 3 -0.9612163945517977 4 4 -1.5372424384535037 4 5 -0.06253328631603239 4 6 -0.34045317221912086 4 7 0.18788028786174493 4 8 0.46724755513065475 4 9 0.39708727382355846 4 10 0.12436233920413997 4 11 0.12137370030969663 4 12 0.3591636956441633 4 13 -1.297690697696865 4 14 0.7824886591137172 4 15 0.3007061270963902 4 16 0.5735069593448188 4 17 0.8115503290305597 4 18 -1.4477041344441233 4 19 -1.9492885842119492 4 20 -2.144475022286443 4 21 -0.5673028336523132 4 22 -1.2764981709123973 4 23 -2.308044522484714 4 24 -1.3798304615963985 4 25 -1.06324782552538 4 26 -0.7353121565368284 4 27 -0.3842705030730258 4 28 0.4698882343284225 4 29 0.23021265067114172 4 30 -0.007302445865228435 4 31 -1.6359135358517563 4 32 -1.511221079683743 4 33 -2.7400476555629822 4 34 -0.4581634539185231 4 35 0.34528872635026886 4 36 1.424004858353598 4 37 1.2433327327914583 4 38 1.8523819784323976 4 39 0.9652035852048186 4 40 1.5220873945354825 4 41 0.22130976825118864 4 42 -0.2421733625577005 4 43 -0.2253877870907104 4 44 0.6187880582097094 4 45 1.426150422461589 4 46 2.2833009900792796 4 47 2.72694428450113 4 48 3.4883418073111656 4 49 1.8965766925171224 4 50 3.115942530858497 4 51 2.5944339575951147 4 52 2.7696882523748556 4 53 1.6277258263788212 4 54 3.500772420582102 4 55 0.9557592041341358 4 56 -0.4052499355699979 5 1 0.2311774181769124 5 2 0.7388615099131712 5 3 1.2404642777171162 5 4 1.2694802506221237 5 5 1.2231809993982523 5 6 1.0545048109741515 5 7 0.9651912122314883 5 8 0.8538021769793929 5 9 0.8550704671008731 5 10 0.9436900702965829 5 11 0.3020459692172537 5 12 -0.208063195112139 5 13 0.5594521594459891 5 14 1.0766063061725433 5 15 0.8595296565081583 5 16 0.03569183329440051 5 17 0.6812982281902222 5 18 -0.2418217815029493 5 19 0.7776021720905675 5 20 0.24628128023456952 5 21 -0.06730283365231315 5 22 0.7865270391716308 5 23 1.839014301044699 5 24 0.6873964291599037 5 25 0.15944125010487653 5 26 1.449561793043003 5 27 0.5653093288597475 5 28 1.0077033603788408 5 29 0.9024815582341716 5 30 1.0851345289246837 5 31 2.0573637750726164 5 32 1.13583774384567 5 33 1.3103725125042445 5 34 2.5292315040646756 5 35 0.4083139364342969 5 36 -0.9247346374447218 5 37 -1.441541216788373 5 38 -0.6728281056012193 5 39 -2.8793342299212306 5 40 -0.15858487437207458 5 41 -2.0055809880513316 5 42 -2.3892321860871135 5 43 -3.3598415686033185 5 44 -1.8644052191012115 5 45 -1.5570428548493318 5 46 -2.115858673786267 5 47 -0.9117111776837419 5 48 -1.599893486806483 5 49 -0.700061962945064 5 50 -1.900864191830582 5 51 -1.0568265466065618 5 52 -2.9025806551881743 5 53 -2.935299383705214 5 54 -2.0076309407624358 5 55 -1.6114676866221664 5 56 0.41407779552243795 6 1 -0.46686179750935963 6 2 -0.6846679018515331 6 3 -0.8183592516946518 6 4 -0.6324805336915986 6 5 -0.8434856672684177 6 6 -0.43569126745721576 6 7 -0.8073578073763557 6 8 -0.875609587726494 6 9 -1.0743412976050095 6 10 -0.7661138512720456 6 11 -1.0548167758807807 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3.5705829692359607 6 54 5.41982003962972 6 55 2.7414734898484205 6 56 1.7709405406204795 7 1 1.1135303593533834 7 2 1.7976850393249393 7 3 1.3581113365406452 7 4 2.2694802506221237 7 5 1.6349457052806073 7 6 2.1133283403859195 7 7 1.4357794475256114 7 8 1.4420374710970378 7 9 1.502129290630286 7 10 2.29663124676717 7 11 1.7726342045113768 7 12 1.791936804887861 7 13 1.4418051006224601 7 14 1.6648416002901882 7 15 1.5654120094493322 7 16 0.7415741862355745 7 17 0.7401217576019903 7 18 1.1699429243794057 7 19 0.7776021720905675 7 20 1.1874577508228015 7 21 1.4621089310535709 7 22 1.6100564509363409 7 23 0.13313194810351803 7 24 1.0403376056304907 7 25 0.6300294853989925 7 26 1.6260323812783 7 27 -0.9052789064343685 7 28 0.0077033603788407845 7 29 0.9024815582341716 7 30 2.0851345289246837 7 31 2.6455990691902613 7 32 2.606425979139786 7 33 1.5456666301513025 7 34 2.4115844452411395 7 35 3.584784524669594 7 36 3.01644183314351 7 37 3.440811724388098 7 38 4.033054247339955 7 39 1.767724593608179 7 40 3.9590621844514544 7 41 3.582654306066317 7 42 1.7872384021481835 7 43 2.346040784337859 7 44 1.782653604428198 7 45 2.2076630275036067 7 46 0.884141326213733 7 47 2.205935881139787 7 48 1.9295182778993976 7 49 0.8293498017608201 7 50 1.8638416905223636 7 51 1.7078793357463766 7 52 2.6856546389294778 7 53 1.947053557471257 7 54 3.1688396474728577 7 55 3.5061793722013626 7 56 1.296430736698909 8 1 -1.4001951308426968 8 2 -1.1513345685182017 8 3 -1.5516925850279861 8 4 -0.03248053369160431 8 5 -3.2434856672684163 8 6 -1.0356912674572172 8 7 -2.1406911407096842 8 8 -2.0756095877264897 8 9 -2.0076746309383466 8 10 -0.7661138512720456 8 11 -0.4548167758807793 8 12 -0.38845535197488346 8 13 -0.7072145072206766 8 14 -0.5508446742196185 8 15 -1.3992938729036126 8 16 -1.0074454216075637 8 17 -0.7932115757313412 8 18 -1.0810374677774561 8 19 -1.4302409651643302 8 20 0.003144025332602496 8 21 0.4660304996810183 8 22 0.08064468623045684 8 23 0.1252888108486161 8 24 0.1344552526893139 8 25 -0.4013430636206152 8 26 -1.6210264422511145 8 27 -1.269984788787312 8 28 0.7841739486141392 8 29 0.17306979352828478 8 30 1.1165070779442914 8 31 1.692657892719673 8 32 1.0221122536495884 8 33 -0.45433336984869754 8 34 0.8037413079862432 8 35 0.702431583493123 8 36 0.5144810488297864 8 37 -0.970952981494257 8 38 0.942858168908586 8 39 0.17948929949053394 8 40 0.9316112040592941 8 41 0.6689288158702382 8 42 1.5911599707756352 8 43 0.346040784337859 8 44 1.3473594867811372 8 45 1.7213885176996868 8 46 1.8880628948411804 8 47 0.7745633321201808 8 48 -1.4783248593554994 8 49 1.31562431156474 8 50 -0.04596223104626773 8 51 1.5706244337855928 8 52 1.4268311095177104 8 53 1.1705829692359622 8 54 -0.44684662703694755 8 55 -0.7251931768182445 8 56 1.5709405406204766 9 1 1.1331382024906347 9 2 1.248665431481804 9 3 1.5149740816386839 9 4 2.0341861329750657 9 5 1.489847666064918 9 6 1.2976420658761185 9 7 1.3259755259569843 9 8 1.2577237456068389 9 9 0.8589920357283205 9 10 1.7672194820612859 9 11 1.8785165574525493 9 12 1.4782113146917837 9 13 2.559452159445989 9 14 1.4491553257803815 9 15 1.5340394604297245 9 16 0.9925545783924363 9 17 1.2067884242686588 9 18 1.1856291988892096 9 19 1.436425701502337 9 20 1.469810691999271 9 21 0.5326971663476883 9 22 0.8139780195637911 9 23 1.4586221441819518 9 24 -0.1988780806440147 9 25 2.0653236030460533 9 26 0.978973557748887 9 27 1.930015211212691 9 28 1.7841739486141392 9 29 1.2397364601949548 9 30 0.6498404112776228 9 31 1.55932455938634 9 32 0.6887789203162598 9 33 2.3456666301512996 9 34 1.7370746413195732 9 35 2.50243158349312 9 36 -0.4188522845035507 9 37 2.029047018505743 9 38 -0.9904751644247511 9 39 -0.48717736717613036 9 40 -0.3350554626073716 9 41 -1.531071184129761 9 42 -2.4088400292243684 9 43 -1.187292548995476 9 44 -0.7859738465521922 9 45 -2.078611482300314 9 46 -0.17860377182548604 9 47 -0.6254366678798213 9 48 -0.2783248593555001 9 49 -1.2177090217685915 9 50 0.08737110228706513 9 51 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1.7578266374422995 115 43 2.346040784337859 115 44 2.48485948678114 115 45 3.3547218510330197 115 46 0.792229561507849 115 47 4.878729998786845 115 48 4.675841807311166 115 49 5.744790978231407 115 50 3.4998711022870665 115 51 3.6747911004522607 115 52 0.5018311095177097 115 53 3.3330829692359636 115 54 3.1614867062963867 115 55 4.8039734898484205 115 56 2.1126072072871445 116 1 -0.8864696406466166 116 2 -0.9081973136162347 116 3 -0.46541807522406486 116 4 -0.7893432787896373 116 5 -0.8944660594252767 116 6 -1.2396128360846674 116 7 -0.21127937600380164 116 8 -0.3814919406676651 116 9 -0.08610600348736597 116 10 -1.4092511061740112 116 11 -1.2273657954886232 116 12 -1.031592606876849 116 13 -0.7934890170245978 116 14 0.19425336499607226 116 15 0.33011789180227424 116 16 -0.19960228435266458 116 17 1.0342394046608163 116 18 1.3464135126147028 116 19 0.7776021720905675 116 20 0.4815753978816275 116 21 1.1679912839947448 116 22 1.4335858627010438 116 23 0.9566613598682281 116 24 1.7462199585716718 116 25 1.3359118383401665 116 26 0.5672088518665319 116 27 0.9182505053303345 116 28 1.1253504192023698 116 29 1.2554227347047586 116 30 -1.2678066475459033 116 31 -0.2955774013979706 116 32 -0.04063284438962711 116 33 2.6044901595630705 116 34 -0.1766508488765055 116 35 -1.356391945918645 116 36 -3.101205225680019 116 37 -3.2650706285530795 116 38 -2.4375339879541613 116 39 -3.7616871710977016 116 40 -2.158584874372078 116 41 -2.182051576286625 116 42 -1.565702774322407 116 43 -2.8304298038974345 116 44 -0.982052277924744 116 45 -0.9099840313199223 116 46 -0.40997632084509306 116 47 -1.0293582365072709 116 48 0.2236359249582236 116 49 -1.17065019823918 116 50 1.393253455228244 116 51 1.9431734533934382 116 52 2.038595815400061 116 53 1.7705829692359636 116 54 2.1688396474728577 116 55 2.682649960436656 116 56 1.3552542661106735 117 1 -1.7835284641760296 117 2 -2.7721679018515317 117 3 -4.193359251694652 117 4 -4.715813867024934 117 5 -3.5268190006017477 117 6 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119 7 -0.11569114070968567 119 8 0.13689041227350884 119 9 0.13815870239498906 119 10 -1.1702805179387141 119 11 0.04101655745255073 119 12 0.019877981358447983 119 13 -0.6905478405540109 119 14 -0.8425113408862828 119 15 -0.6367938729036098 119 16 -0.7657787549408965 119 17 0.4901217576019903 119 18 0.5522958655558767 119 19 -0.22607429849766447 119 20 -0.8051893080007275 119 21 0.5576971663476868 119 22 0.01814468623045684 119 23 0.7544554775152861 119 24 0.5219552526893168 119 25 0.4903236030460505 119 26 0.22897355774888695 119 27 -0.1699847887873105 119 28 0.43417394861413783 119 29 0.8730697935282876 119 30 -0.27515958872237434 119 31 1.7301578927196744 119 32 1.426278920316257 119 33 0.17066663015130246 119 34 1.6579079746529075 119 35 1.577431583493123 119 36 2.968647715496452 119 37 2.529047018505743 119 38 3.3970248355752517 119 39 3.179489299490534 119 40 0.7899445373926284 119 41 0.9980954825369039 119 42 0.6953266374422995 119 43 1.471040784337859 119 44 2.17235948678114 119 45 0.10472185103301968 119 46 2.604729561507849 119 47 3.628729998786845 119 48 2.4258418073111656 119 49 3.682290978231407 119 50 1.3123711022870665 119 51 3.1747911004522607 119 52 1.4393311095177097 119 53 0.5830829692359636 119 54 -0.40101329370361327 119 55 -0.8210265101515795 119 56 -0.7623927927128555 120 1 -0.18058728770544263 120 2 -0.5552561371456477 120 3 -1.1124768987534779 120 4 0.38712730944565266 120 5 -0.7179954711899796 120 6 0.9956812815623906 120 7 -1.0936323171802727 120 8 -1.2638448818441361 120 9 -0.9096354152520689 120 10 -0.4680746355857721 120 11 -1.0508952072533333 120 12 -0.443357312759197 120 13 -0.9699596052598949 120 14 -0.04104075265098572 120 15 -0.43458799055066777 120 16 -0.08195522552912848 120 17 0.32835705171963525 120 18 0.22876645379117377 120 19 0.6011315838552775 120 20 -0.34195401388308255 120 21 0.5797559898770999 120 22 0.2571152744657539 120 23 -0.6903974636611849 120 24 0.8050434879834327 120 25 0.6888530148107535 120 26 0.5672088518665319 120 27 -0.14057302408142647 120 28 -0.05112016903292016 120 29 -0.6857537358834804 120 30 1.4968992348070387 120 31 1.8220696574255584 120 32 1.13583774384567 120 33 1.0750783948571865 120 34 1.9998197393587915 120 35 1.231843348199007 120 36 2.310559480202336 120 37 1.617282312623388 120 38 0.9742307179281937 120 39 1.708901064196418 120 40 1.3708268903338023 120 41 0.994419011948672 120 42 1.3166501668540675 120 43 -0.35984156860331495 120 44 2.900300663251727 120 45 1.5606042039741936 120 46 1.060611914449023 120 47 2.147112351728019 120 48 0.7530476896641076 120 49 2.594055684113762 120 50 3.3932534552282405 120 51 2.8255263945699056 120 52 0.5091840506941807 120 53 1.4176417927653766 120 54 2.2864867062963867 120 55 3.447355842789598 120 56 3.17878367787538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 75.0 5 8.0 6 3.0 7 7.0 8 14.0 9 20.0 10 17.0 11 17.0 12 38.0 13 45.0 14 56.0 15 58.0 16 80.0 17 91.0 18 125.0 19 112.0 20 129.0 21 135.0 22 154.0 23 160.0 24 203.0 25 298.0 26 233.0 27 297.0 28 358.0 29 398.0 30 443.0 31 526.0 32 631.0 33 798.0 34 1016.0 35 1314.0 36 1749.0 37 2590.0 38 3051.0 39 2593.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.02477300751038 0.3250756641632104 0.5212420132272166 0.1289093150992041 8 98.51449072257414 0.45966702169404117 0.8800941756824934 0.14574808004933013 9 97.67963232821432 1.1713933415536375 0.3923326981280125 0.7566416321040242 10 51.40118820760004 26.92523259724246 8.491200538056273 13.182378657101221 11 23.074767402757537 37.91054814482681 23.539961887680754 15.474722564734893 12 46.659567313081496 16.612487389306132 21.24201322721668 15.485932070395695 13 24.30220827261518 15.009528079811679 35.388409371146736 25.29985427642641 14 33.45476964465867 21.987445353659904 24.9915928707544 19.566192130927025 15 25.490415872660016 19.532563613944625 23.467100100885553 31.509920412509807 16 22.503082614056723 29.974218136980156 26.835556551956056 20.68714269700706 17 38.201995292007624 18.927250308261407 19.426073310167023 23.44468108956395 18 24.10043717072077 18.39479878937339 36.16747001457236 21.337294025333485 19 36.17867952023316 18.450846317677392 23.293352763143147 22.077121398946307 20 22.637596681986324 18.624593655419797 26.224638493442438 32.51317116915144 21 25.047640399058402 16.029593094944513 36.92411164667638 21.998654859320705 22 26.476852370810448 17.380338527070958 26.79071852931286 29.35209057280574 23 28.48335388409371 20.34525277435265 33.976011657885884 17.19538168366775 24 37.367565446493636 21.59313862884691 24.743539436067046 16.29575648859241 25 28.98940642340676 19.982063785662238 32.98021411355866 18.048315677372344 26 37.76431656290313 19.131751640585563 24.14605418138987 18.957877615121433 27 28.7020417321068 17.797846084810413 32.33116446039937 21.16894772268342 28 35.8080808080808 22.586980920314254 24.21436588103255 17.39057239057239 29 36.955300988319856 21.759883198562445 22.44496855345912 18.83984725965858 30 31.893015676799465 24.17261336180255 26.4932291959319 17.44114176546609 31 28.271014900196796 26.747258926061292 27.663761596851277 17.31796457689064 32 24.07105514643881 26.60632975434257 30.136601270448033 19.186013828770587 33 25.400753698183248 27.779965127397492 27.959952753248213 18.859328421171046 34 21.936536514009227 28.77236412737707 30.96658039833465 18.32451896027906 35 23.08904649330181 30.018011932905548 30.575256107171 16.317685466621636 36 21.572160594628077 30.153724871895943 28.638999943690525 19.63511458978546 37 21.289777527457055 31.6192621796677 29.597296536186988 17.493663756688257 38 22.94038093091401 28.834666967203876 31.18449227995041 17.0404598219317 39 20.76120665351001 29.275444037214548 30.228361996053003 19.73498731322244 40 21.103401590793705 29.237885711062223 32.43075534495403 17.22795735319005 41 20.919605077574047 29.754583921015517 30.691114245416077 18.63469675599436 42 19.159142212189618 28.560948081264108 33.88261851015801 18.397291196388263 43 22.0214568040655 29.59345002823264 32.86278938452851 15.52230378317335 44 22.295618789521228 28.06007226738934 34.276196928635954 15.368112014453478 45 20.496017174170948 31.9247500141235 29.574600305067513 18.004632506638043 46 19.367052839785252 29.52811528680418 32.67589714608646 18.428934727324105 47 21.188645102917892 31.49739877855689 31.078941415969236 16.235014702555983 48 21.007975564228744 30.346739068951862 31.879631200859777 16.765654165959614 49 24.212609556121006 30.20073508623127 30.392988408255583 15.19366694939214 50 20.193504583003282 32.539323299762366 29.653728640941495 17.613443476292858 51 19.58477117157889 32.765740793121005 31.102562652033715 16.54692538326639 52 18.338708764782435 33.740734453686414 32.16771346121202 15.752843320319132 53 19.714803078315978 34.50090538705297 30.04753282028067 15.736758714350385 54 19.078834862908284 34.32138174770564 29.98346918999031 16.61631419939577 55 19.81543339183604 35.124271075128796 29.496687991847363 15.563607541187793 56 19.846031925733048 32.79180346428167 29.367145930035093 17.995018679950185 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 2.5 9 4.0 10 13.5 11 23.0 12 34.5 13 55.5 14 65.0 15 54.0 16 43.0 17 75.5 18 108.0 19 134.5 20 161.0 21 145.0 22 161.0 23 193.0 24 240.5 25 288.0 26 356.5 27 425.0 28 501.5 29 564.0 30 550.0 31 632.5 32 715.0 33 671.0 34 627.0 35 696.5 36 766.0 37 624.5 38 466.0 39 449.0 40 425.5 41 402.0 42 466.5 43 531.0 44 521.0 45 511.0 46 606.0 47 702.5 48 704.0 49 769.0 50 834.0 51 753.5 52 673.0 53 728.0 54 791.5 55 800.0 56 777.0 57 754.0 58 823.0 59 892.0 60 1031.0 61 1170.0 62 1093.5 63 924.5 64 832.0 65 627.0 66 422.0 67 375.5 68 329.0 69 320.0 70 311.0 71 241.5 72 143.5 73 115.0 74 107.5 75 100.0 76 79.5 77 59.0 78 45.0 79 28.0 80 25.0 81 18.0 82 11.0 83 8.5 84 6.0 85 3.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.01681425849120054 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.01681425849120054 25 0.005604752830400179 26 0.07286178679520233 27 0.07846653962560252 28 0.12330456226880394 29 0.19056159623360608 30 0.25221387736800804 31 0.3194709113328102 32 0.2970519000112095 33 0.3530994283152113 34 0.38112319246721216 35 0.4259612151104136 36 0.4651944849232149 37 0.4876134962448156 38 0.5380562717184173 39 0.5997085528528192 40 0.6445465754960207 41 0.6557560811568209 42 0.6837798453088219 43 0.7398273736128237 44 0.7286178679520233 45 0.7902701490864253 46 0.8238986660688263 47 0.8855509472032284 48 0.9135747113552293 49 0.8799461943728281 50 0.9415984755072302 51 0.9247842170160296 52 0.9472032283376304 53 0.9528079811680304 54 1.6758210962896536 55 1.0032507566416322 56 0.9864364981504316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 17842.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.60475283040019 #Duplication Level Percentage of deduplicated Percentage of total 1 72.94627557705876 40.561596233606096 2 14.020764035883479 15.592422374173298 3 5.291805261566374 8.827485707880282 4 2.7517387360145147 6.120390090796996 5 1.4917851023082351 4.147517094496133 6 0.9676443906864227 3.228337630310503 7 0.554379598830763 2.157829839704069 8 0.383025904646709 1.7038448604416545 9 0.3023888720895071 1.5132832642080485 >10 1.1793166011490779 10.699473153233942 >50 0.09071666162685213 3.884093711467324 >100 0.020159258139300473 1.56372603968165 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGTATAC 178 0.997646003811232 No Hit CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 101 0.5660800358704181 No Hit CTGTGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 94 0.5268467660576168 No Hit CTGTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 93 0.5212420132272166 No Hit CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 88 0.4932182490752158 No Hit CTGTGTGGGATCCTTTCTTGTTATTCCTCTTCCCTCACCCCTACCTACTCCATTCT 83 0.4651944849232149 No Hit CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 81 0.45398497926241455 No Hit CTGTGTGGGAAGCAAAAGCGATTGCCTTAGTAGCGGCGAGCGAAACGGCAGGGGGG 71 0.39793745095841276 No Hit CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 70 0.3923326981280125 No Hit CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.3250756641632104 No Hit CTGTGTGGGGAACAAAATAATCCCATTAATATCTTTCTTGGATTCTCAGCAGTTTT 55 0.3082614056720099 No Hit CTGTGTGGGATGGAGAACCCTGACATGGGGGCAGGAACCCCCATACCCTGCCACCA 49 0.2746328886896088 No Hit CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.22419011321600715 No Hit CTGTGTGGGGTTGGCCAGACTTAACTCTGCTCCCTTGACCACAGAGATGAGATTCT 39 0.218585360385607 No Hit CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTGTTTGTT 38 0.2129806075552068 No Hit CTGTGTGGGGGAAGAACGTCTGCTCAGATGGACCCTGAGATGTCTCCACGGACTTT 36 0.20177110189440645 No Hit CTGTGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 35 0.19616634906400626 No Hit CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.19056159623360608 No Hit CTGTGTGGGGTCTCCATTGGTAGCGTAACGAGCTCCTTCCATAGCCTTGGAAATAG 33 0.18495684340320592 No Hit CTGTGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 32 0.17935209057280574 No Hit CTGTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 32 0.17935209057280574 No Hit CTGTGTGGGATTGGGGGAGGAGATGACTCCTTCAACACCTTCTTCAGTGAGACAGG 31 0.17374733774240556 No Hit CTGTGTGGGGAAACTTTTTTTTCAGTTCTTGAGACAGGGTCTCACTATGTCACCTT 31 0.17374733774240556 No Hit CTGTGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 30 0.16814258491200537 No Hit CTGTGTGGGGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.15693307925120503 No Hit CTGTGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.15693307925120503 No Hit CTGTGTGGGGTTAAAAGGAACAGAATACAAAGTCTCTGTAATTCATAGAGCTAAAT 28 0.15693307925120503 No Hit CTGTGTGGGCTAAGGGGAGAGGCTCAGAGGAAAATGGCAAAGCCTTCCTCTGGACC 26 0.14572357359040466 No Hit CTGTGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 25 0.14011882076000448 No Hit CTGTGTGGGGTGTGGGCAGGGGTGTGGGGGATAACCTGTGAGTGCAGCCGGGTTGG 25 0.14011882076000448 No Hit CTGTGTGGGGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGG 23 0.1289093150992041 No Hit CTGTGTGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGCGGAC 22 0.12330456226880394 No Hit CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.11769980943840377 No Hit CTGTGTGGGGGTGGGCGGAACACATCGTCTCTCCGCCATGGCGGCCCTGCGTGCTC 21 0.11769980943840377 No Hit CTGTGTGGGCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC 21 0.11769980943840377 No Hit CTGTGTGGGGGCCTTAGACTGGAATTGGCTGTCTCGGGGCTCTGGCCGCCGGCCTC 21 0.11769980943840377 No Hit CTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.11209505660800358 No Hit CTGTGTGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC 20 0.11209505660800358 No Hit CTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.1064903037776034 No Hit CTGTGTTTGAAGCTTGCCGGTAGTGTTGCTTTGAGTTACAGTATAGCCTGCGTTTA 19 0.1064903037776034 No Hit CTGTGTGGGATCTCTGCCCAGGACTCTCAGTGGGAGCTCCAGCTGACGAGGTAAGG 19 0.1064903037776034 No Hit CTGTGTGGGCGGGTCGTGCCTTTGCATCGCTGAAGGAACTTTTTGTCTTTTAAGGA 19 0.1064903037776034 No Hit CTGTGTGGGGGTCTGAGTCCTGGCCGAGGAACTTCTGTTGGTGTTTGGGGCCGCAG 19 0.1064903037776034 No Hit CTGTGTGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.10088555094720322 No Hit CTGTGTGGGACTCTTGTCAGTTATTAAACAAACAAACAGAAAAAAATTGTTTTGAA 18 0.10088555094720322 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.005604752830400179 0.0 0.0 0.0 0.0 9 0.005604752830400179 0.0 0.0 0.0 0.0 10 0.005604752830400179 0.0 0.0 0.0 0.0 11 0.022419011321600717 0.0 0.0 0.0 0.0 12 0.028023764152000898 0.0 0.0 0.0 0.0 13 0.028023764152000898 0.0 0.0 0.0 0.0 14 0.033628516982401074 0.0 0.0 0.0 0.0 15 0.05044277547360161 0.0 0.0 0.0 0.0 16 0.06165228113440197 0.0 0.0 0.0 0.0 17 0.06165228113440197 0.0 0.0 0.0 0.0 18 0.06165228113440197 0.0 0.0 0.0 0.0 19 0.07846653962560252 0.0 0.0 0.0 0.0 20 0.07846653962560252 0.0 0.0 0.0 0.0 21 0.08967604528640287 0.0 0.0 0.0 0.0 22 0.1064903037776034 0.0 0.0 0.0 0.0 23 0.12330456226880394 0.0 0.0 0.0 0.0 24 0.1289093150992041 0.0 0.0 0.0 0.0 25 0.1345140679296043 0.0 0.0 0.0 0.0 26 0.14572357359040466 0.0 0.0 0.0 0.0 27 0.14572357359040466 0.0 0.0 0.0 0.0 28 0.15693307925120503 0.0 0.0 0.0 0.0 29 0.15693307925120503 0.0 0.0 0.0 0.0 30 0.17374733774240556 0.0 0.0 0.0 0.0 31 0.17935209057280574 0.0 0.0 0.0 0.0 32 0.17935209057280574 0.0 0.0 0.0 0.0 33 0.1961663490640063 0.0 0.0 0.0 0.0 34 0.20177110189440645 0.0 0.0 0.0 0.0 35 0.22979486604640736 0.0 0.0 0.0 0.0 36 0.23539961887680752 0.0 0.0 0.0 0.0 37 0.23539961887680752 0.0 0.0 0.0 0.0 38 0.2466091245376079 0.0 0.0 0.0 0.0 39 0.25221387736800804 0.0 0.0 0.0 0.0 40 0.2690281358592086 0.0 0.0 0.0 0.0 41 0.28023764152000896 0.0 0.0 0.0 0.0 42 0.28584239435040915 0.0 0.0 0.0 0.0 43 0.29144714718080933 0.0 0.0 0.0 0.0 44 0.3026566528416097 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTTCC 15 0.008123607 50.264206 34 AAACTCT 15 0.008123607 50.264206 34 TAGCCTT 15 0.008123607 50.264206 42 CGCCATG 15 0.008123607 50.264206 34 GCGGCCC 15 0.008123607 50.264206 41 ACGTACT 15 0.008123607 50.264206 41 GAGCTCC 15 0.008123607 50.264206 30 ACTCTAC 15 0.008123607 50.264206 36 TCTACGT 20 3.9672165E-4 50.264206 38 CTCTACG 15 0.008123607 50.264206 37 AACTCTA 15 0.008123607 50.264206 35 CTTGGAA 15 0.008123607 50.264206 46 TACGTAC 15 0.008123607 50.264206 40 TTCCATA 15 0.008123607 50.264206 37 CGTACTG 15 0.008123607 50.264206 42 CCTTCCA 15 0.008123607 50.264206 35 CTACGTA 15 0.008123607 50.264206 39 CATAGCC 15 0.008123607 50.264206 40 TCTCCGC 15 0.008123607 50.264206 30 CATGGCG 15 0.008123607 50.264206 37 >>END_MODULE