FastQCFastQC Report
Fri 17 Jun 2016
SRR1526898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71378
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.39087674073243855No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.3796688055143041No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.2886043318669618No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC980.13729720642214688No Hit
GGGCGAGGGATAGCAGTCATTTTTATTGTCAATGACTGCTTATTTTTCTTCAGAGGAAATGACC960.13449522261761326No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.12608927120401242No Hit
GGGCGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC830.1162823278881448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTATA150.004514577358.49504558
AGGTCCG150.004514577358.49504558
AGGGTGG1350.058.124566
CGAGGAT201.976434E-458.1245574
TGATCGC150.00466855758.0021142
GTCATTT150.00466855758.0021116
AACCGTG150.00466855758.0021153
GATCGCG150.00466855758.0021143
CGTTGTA150.00466855758.0021113
GCAGTCA150.00466855758.0021113
CAACCGT150.00466855758.0021152
GCAAAAC150.00466855758.0021146
GTGATGA150.00466855758.0021114
TAGTTTT150.00466855758.0021152
GTATGCA150.00466855758.0021117
CTCATCC303.7105383E-758.0021114
ACAGAAC150.00466855758.0021115
TTTGACC150.00466855758.0021145
GAACACT150.00466855758.0021115
AAAATGA150.00466855758.0021147
Produced by FastQC (version 0.11.5)