FastQCFastQC Report
Fri 17 Jun 2016
SRR1526897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101018
Sequences flagged as poor quality0
Sequence length64
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC5930.5870240947157932No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.45140470015244805No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3370.333603912174068No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3290.3256845314696391No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3210.31776515076521017No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3070.3039062345324596No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.2930170860638698No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2830.2801480924191728No Hit
GGGCGAGGGGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTGCCCAGCTTAGTGCATT2650.26232948583420773No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2380.23560157595676018No Hit
GGGCGAGGGGGCTGCCTCCATGCTGTCTCTTGGGCCGGCTCCTTTTCTGAGTGTAGTGGAGGGC1810.17917598843770416No Hit
GGGCGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1600.15838761408857827No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1420.14056900750361323No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1370.13561939456334515No Hit
GGGCGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1360.13462947197529154No Hit
GGGCGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1270.125720168682809No Hit
GGGCGAGGGGATCAATTTAAGTCATTGTTAGTTTGTATCCCAAGAGGATTGTGTTTTGCAATGT1260.12473024609475539No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT1140.11285117503811201No Hit
GGGCGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1140.11285117503811201No Hit
GGGCGAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC1130.1118612524500584No Hit
GGGCGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1130.1118612524500584No Hit
GGGCGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT1120.11087132986200479No Hit
GGGCGAGGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGC1100.10889148468589756No Hit
GGGCGAGGGAGCTCACCAGACTTGCACAGCAAGGACCTTGCTCACTCAGCCATCTCATCAACCC1100.10889148468589756No Hit
GGGCGAGGGATAGCAGTCATTTTTATTGTCAATGACTGCTTATTTTTCTTCAGAGGAAATGACC1100.10889148468589756No Hit
GGGCGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1100.10889148468589756No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1070.10592171692173673No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1050.1039418717456295No Hit
GGGCGAGGGGTACGTTGTATGCACTTTATCTAAAAGGGAGATGATCGCGAAAAGGAGCCCATTG1020.10097210398146866No Hit
GGGCGAGGGGTGCAGTGCTGGGCGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG1020.10097210398146866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAGC201.9633606E-458.22620857
GTTTCGA201.9633606E-458.22620856
TCGGGCA150.004603311458.22620855
TTCGACG201.9633606E-458.22620858
AAATAGC150.004621512358.16824347
TACTCTT150.004621512358.16824346
CCTTGAA150.004621512358.16824348
CGCTTGT150.004621512358.16824345
GTGCCCT150.004621512358.16824345
TTTAACA201.9730601E-458.1682447
CTCGATC201.9730601E-458.1682444
CGATCCA201.9730601E-458.1682446
GCTTTAA201.9730601E-458.1682445
GTTACGA150.004639766658.11039741
TTACGAG150.004639766658.11039742
CACATGC201.9827977E-458.1103939
ATGCAAG201.9827977E-458.1103942
AGAAATT150.00464891458.08151238
ACACTTG501.8189894E-1258.0526636
CGGTATT150.004658074658.05265836
Produced by FastQC (version 0.11.5)