FastQCFastQC Report
Fri 17 Jun 2016
SRR1526896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100774
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC5720.5676067239565761No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.3899815428582769No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3120.3096036676126779No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2960.29372655645305334No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2950.2927342370055768No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.27784944529342886No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2560.2540337785539921No Hit
GGGCGAGGGGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTGCCCAGCTTAGTGCATT2530.2510568202115625No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2410.23914898684184416No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2390.23716434794689104No Hit
GGGCGAGGGGGCTGCCTCCATGCTGTCTCTTGGGCCGGCTCCTTTTCTGAGTGTAGTGGAGGGC2050.20342548673268895No Hit
GGGCGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1560.15480183380633894No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1450.14388631988409709No Hit
GGGCGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1450.14388631988409709No Hit
GGGCGAGGGATAGCAGTCATTTTTATTGTCAATGACTGCTTATTTTTCTTCAGAGGAAATGACC1280.12701688927699606No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT1250.12403993093456646No Hit
GGGCGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1170.1161013753547542No Hit
GGGCGAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC1140.1131244170123246No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1120.11113977811737154No Hit
GGGCGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT1060.10518586143251235No Hit
GGGCGAGGGGTGCAGTGCTGGGCGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG1040.10320122253755928No Hit
GGGCGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1040.10320122253755928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAGGT201.9180118E-458.50151445
TATGCAT150.004536058858.44265741
ATCTTCG150.004545105658.41327326
ATTGTTA150.00455416658.38392324
TTGTTAG150.00455416658.38392325
ATCAGGT150.00457232758.32530221
TCACAAC201.9858491E-458.09200346
CACAACC201.9858491E-458.09200347
GCCCACC150.004645511558.09255
GATTTTG150.004645511558.09258
TTAGTGC453.092282E-1158.09255
CCGCGAA150.004645511558.09250
TAGTGCA453.092282E-1158.09256
TCGACGC303.6862548E-758.09258
AGTAAGC150.004645511558.09256
TGCTTAT303.72469E-758.00499337
ACCATAT150.004673179258.00499337
TTAGAGA202.0006308E-458.00499335
GTCAGAT150.004673179258.00499337
CGCGGTA150.004700969457.91824731
Produced by FastQC (version 0.11.5)