FastQCFastQC Report
Fri 17 Jun 2016
SRR1526895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91104
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5540.6080962416578856No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4700.5158939234281701No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3840.42149631190727077No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.3863716192483316No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2880.31612223393045313No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.27002107481559534No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2250.2469704952581665No Hit
GGGCGAGGGGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTGCCCAGCTT2180.23928696873902353No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1990.2184316824727784No Hit
GGGCGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1700.18659992975061468No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1450.1591587636108184No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1420.15586582367404286No Hit
GGGCGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1400.15367053038285916No Hit
GGGCGAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC1360.14927994380049175No Hit
GGGCGAGGGGGCTGCCTCCATGCTGTCTCTTGGGCCGGCTCCTTTTCTGAGTGTAG1330.14598700386371619No Hit
GGGCGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1230.13501053740779767No Hit
GGGCGAGGGGGAGTGTTTCAGAGAATCACTTGGTGGCAGTCACGTGGCTGACTCCT1210.132815244116614No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1190.1306199508254303No Hit
GGGCGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1180.12952230417983843No Hit
GGGCGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1100.12074113101510361No Hit
GGGCGAGGGCATCTTCTGTATGTATCCCACAGTAAGTTCACATGCAAGCTCCACAC1090.11964348436951176No Hit
GGGCGAGGGATAGCAGTCATTTTTATTGTCAATGACTGCTTATTTTTCTTCAGAGG1080.11854583772391993No Hit
GGGCGAGGGAGCTCACCAGACTTGCACAGCAAGGACCTTGCTCACTCAGCCATCTC1080.11854583772391993No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1060.11635054443273622No Hit
GGGCGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1050.11525289778714437No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC990.10866701791359325No Hit
GGGCGAGGGGTTTCATATAGTATGAAATTCTCTTAGTAGAATAATACATCTATACT940.103178784685634No Hit
GGGCGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.103178784685634No Hit
GGGCGAGGGGTGCAGTGCTGGGCGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGA930.10208113804004214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTTC150.00797288650.67336749
AATTGTT150.00822229250.28033447
AAATTGT150.00822229250.28033446
ACCGTGC150.00822229250.28033446
TGAGAAT150.00822229250.28033444
ATTTGCC204.0499985E-450.2803344
AATCTCC150.00827648250.19690341
TTGGATT204.094523E-450.16915540
CATTGTG204.094523E-450.16915540
GCCGCAT150.00829460550.16915540
CCACTTA150.00829460550.16915540
AATCTGT150.00829460550.16915540
TCACATG150.00831275650.14143838
GTCCCAC150.00831275650.14143838
AACACAA150.00831275650.14143837
TAAAGGC150.00834914950.08609434
ATGTCCC150.00834914950.08609436
GGCTCGT301.0248932E-650.08609436
AATGTCC150.00834914950.08609435
CGACACT204.1281708E-450.0860934
Produced by FastQC (version 0.11.5)