FastQCFastQC Report
Fri 17 Jun 2016
SRR1526894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20893
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.7801656057052601No Hit
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1200.5743550471449768No Hit
ACACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA1090.5217058344900206No Hit
ACACCCGGGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTATAGTATATTCACA810.38768965682285933No Hit
ACACCCGGGGTGTAACCTTTAGAATTATTTGCAGAATATCCTTTTAGCTTATATGTGAATTAGA620.29675010769157134No Hit
ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA580.2776049394534054No Hit
ACACCCGGGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGT400.19145168238165894No Hit
ACACCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC370.1770928062030345No Hit
ACACCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT350.16752022208395156No Hit
ACACCCGGGCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGAGTTCAGG340.16273393002441008No Hit
ACACCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.14837505384578567No Hit
ACACCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.1388024697267027No Hit
ACACCCGGGCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTC270.12922988560761978No Hit
ACACCCGGGCATGTCCCTGTTTATCTTTAATGTAAACCATGCACAAAACTCAGCAGACTCCTTG270.12922988560761978No Hit
ACACCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11965730148853684No Hit
ACACCCGGGGGTTTCCACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACC240.11487100942899535No Hit
ACACCCGGGAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.11487100942899535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAT257.9415095E-658.5194258
TCGACGC150.00446197258.51941358
TTCGACG150.00446197258.51941357
TAAGCCA351.5184924E-858.09638653
ATTAAGC406.530172E-1058.09638651
GTCTCAA351.5184924E-858.09638642
TCTCAAA351.5184924E-858.09638643
AAGCCAT351.5184924E-858.09638654
AAAGATT406.530172E-1058.09638647
CTCAAAG406.530172E-1058.09638644
TGGTTAT150.004592475558.09638246
TATATTC150.004592475558.09638255
CACAATT150.004592475558.09638242
AATTATC150.004592475558.09638245
TCAATAA150.004592475558.09638255
CATCAAT150.004592475558.09638253
GGTTATA150.004592475558.09638247
AGTATAT150.004592475558.09638253
TAGTATA150.004592475558.09638252
ATAGTAT150.004592475558.09638251
Produced by FastQC (version 0.11.5)