FastQCFastQC Report
Fri 17 Jun 2016
SRR1526893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28883
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3191.104455908319773No Hit
ACACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA3091.0698334660526954No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.9451926738912163No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2480.8586365682235225No Hit
ACACCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2360.8170896375030295No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2140.740920264515459No Hit
ACACCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1640.5678080531800713No Hit
ACACCCGGGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTATAGTATATTCACA1160.40162033029809924No Hit
ACACCCGGGGGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAAT970.33583768999065194No Hit
ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA890.3081397361769899No Hit
ACACCCGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG870.3012152477235744No Hit
ACACCCGGGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGT670.23197036318941938No Hit
ACACCCGGGTTGCCCCATCCCCAAACCTGTCCAGCACTTATGTAAGAACGCATTTGGAGGACAT660.22850811896271161No Hit
ACACCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC620.21465914205588063No Hit
ACACCCGGGGGTTTCCACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACC600.20773465360246512No Hit
ACACCCGGGGGTGCCAGAGAGAGTAGAGGAGAGCAGAGAGCCGTGGAGCGACAGTGGGCAGCAT600.20773465360246512No Hit
ACACCCGGGGTGTAACCTTTAGAATTATTTGCAGAATATCCTTTTAGCTTATATGTGAATTAGA580.20081016514904962No Hit
ACACCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC520.1800366997888031No Hit
ACACCCGGGGGTGCTGGAGCCACTGTCGCCGAGCTCGGGCCACGCTGCTTCTCCTCGCCAGTCG520.1800366997888031No Hit
ACACCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT510.17657445556209533No Hit
ACACCCGGGCATGTGTTTCAAAATAAGGGAACTTCAGTCACCTCAAAGGTAAAATAGTTTAATT500.1731122113353876No Hit
ACACCCGGGCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGAGTTCAGG480.1661877228819721No Hit
ACACCCGGGCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTC450.15580099020184882No Hit
ACACCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.15580099020184882No Hit
ACACCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.1523387459751411No Hit
ACACCCGGGACTGCTTAGATGGTGGGCCTGCCCTCACCAAACAGGAATTTCTATTAAGATTAAG440.1523387459751411No Hit
ACACCCGGGCTTGTGTCAGATGACATCATGGACTCTTCCCTCACTCGCCGGGGACAGATCTGCT430.14887650174843334No Hit
ACACCCGGGGTGGACCTTCCTTCCTCCCATGGCATGTGCAAGTAGGTCTGCGTGACCCCACTTC430.14887650174843334No Hit
ACACCCGGGAGCATTGCAGAGACACCAACTCCAGTAATTGTAGTCACCTCTTCCTTAAGTATGT420.14541425752172557No Hit
ACACCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG410.14195201329501783No Hit
ACACCCGGGATCAAGAAGGTGGTGAAGCAGGCATCTGAGGGCCCACTGAAGGGCATCTTGGGCT400.13848976906831006No Hit
ACACCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA390.13502752484160233No Hit
ACACCCCGTCATTTTGGCCTCGATCGTGGACAGCTACGAACGCCGCAACGAGGGAGCTGCCCGA380.1315652806148946No Hit
ACACCCAATACGATCGATCATGTCCTTATTATGTGCTTATGGTTCATCTATATGTCTTTAAAAT380.1315652806148946No Hit
ACACCCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAG380.1315652806148946No Hit
ACACCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG370.12810303638818682No Hit
ACACCCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC370.12810303638818682No Hit
ACACCCGGGGAACTACTTAGTCAAATGTGATACCATAAATTTAAACAGTTTACCATTTTTCAAT370.12810303638818682No Hit
ACACCCGGGGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAGTGACCTGGACAAGT350.12117854793477131No Hit
ACACCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.11771630370806356No Hit
ACACCCGGGGTGGCACGCTCACTAGTCTGGCTTTGCTGCCCACGAAACGCCACCTCCTGCTGGG340.11771630370806356No Hit
ACACCCAGGGGACACCGTCGTAGAGTCGTCTGGGTTGAACCGCTGGGCCGAAAGCTTTTGTCAG340.11771630370806356No Hit
ACACCCGGGCATGTCCCTGTTTATCTTTAATGTAAACCATGCACAAAACTCAGCAGACTCCTTG340.11771630370806356No Hit
ACACCCGGGGGAATCTAAAGGCCCTCGGCTAGTCTGATCCTTGCCCTAAGCATAGTCCCGTTAG330.11425405948135581No Hit
ACACCCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC320.11079181525464807No Hit
ACACCCGGGAACACATGGCAGTTCAACTTCAAGTTCAACCTGACAGAGGATATGTACTCCCAGG310.10732957102794032No Hit
ACACCCGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA310.10732957102794032No Hit
ACACCCGGGATAAAAATTGTATTAAAAAAAGATATATACTTAGGGGCAAAACCTAAAAGGAAAC300.10386732680123256No Hit
ACACCCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA290.10040508257452481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTAT201.9517426E-458.15331348
TAAGATT201.9517426E-458.15331355
TCTATTA201.9517426E-458.15331350
TTAAGAT201.9517426E-458.15331354
TATTAAG201.9517426E-458.15331352
CGACAGT150.00459264858.1533149
ACAGTGG150.00459264858.1533151
GAGCGAC150.00459264858.1533146
ATCCATG150.00459264858.1533146
GACAGTG150.00459264858.1533150
CGCCTGG258.325798E-658.1533146
GCGACAG150.00459264858.1533148
TAAAATA150.00459264858.1533150
TCTGGCA258.325798E-658.1533153
GTGGGCA150.00459264858.1533154
AAGGTAA150.00459264858.1533146
TGGGCAG150.00459264858.1533155
AAAATAG150.00459264858.1533151
AGGTAAA150.00459264858.1533147
GCCTGGT258.325798E-658.1533147
Produced by FastQC (version 0.11.5)