FastQCFastQC Report
Fri 17 Jun 2016
SRR1526892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29207
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA2961.0134556784332525No Hit
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2650.9073167391378778No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.7977539630910397No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2290.7840586160851851No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2260.7737871058307939No Hit
ACACCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2200.7532440853220118No Hit
ACACCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1360.4656417981990619No Hit
ACACCCGGGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTATAGTATATTCACA1080.36977436915807854No Hit
ACACCCGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1070.36635053240661486No Hit
ACACCCGGGGGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAAT1030.3526551854007601No Hit
ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA900.3081453076317321No Hit
ACACCCGGGCATGTGTTTCAAAATAAGGGAACTTCAGTCACCTCAAAGGTAAAATAGTTTAATT810.2773307768685589No Hit
ACACCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC720.2465162461053857No Hit
ACACCCGGGGGTTTCCACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACC650.22254938884513986No Hit
ACACCCGGGGGTGCCAGAGAGAGTAGAGGAGAGCAGAGAGCCGTGGAGCGACAGTGGGCAGCAT620.21227787859074881No Hit
ACACCCGGGGTGTAACCTTTAGAATTATTTGCAGAATATCCTTTTAGCTTATATGTGAATTAGA600.2054302050878214No Hit
ACACCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC530.1814633478275756No Hit
ACACCCGGGTTGCCCCATCCCCAAACCTGTCCAGCACTTATGTAAGAACGCATTTGGAGGACAT510.1746156743246482No Hit
ACACCCGGGGTGGACCTTCCTTCCTCCCATGGCATGTGCAAGTAGGTCTGCGTGACCCCACTTC500.1711918375731845No Hit
ACACCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT500.1711918375731845No Hit
ACACCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.1677680008217208No Hit
ACACCCGGGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGT480.16434416407025712No Hit
ACACCCCGTCATTTTGGCCTCGATCGTGGACAGCTACGAACGCCGCAACGAGGGAGCTGCCCGA450.15407265381586605No Hit
ACACCCGGGCTTGTGTCAGATGACATCATGGACTCTTCCCTCACTCGCCGGGGACAGATCTGCT440.15064881706440236No Hit
ACACCCGGGCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGAGTTCAGG420.143801143561475No Hit
ACACCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA400.1369534700585476No Hit
ACACCCGGGACTGCTTAGATGGTGGGCCTGCCCTCACCAAACAGGAATTTCTATTAAGATTAAG390.13352963330708392No Hit
ACACCCAGGGGACACCGTCGTAGAGTCGTCTGGGTTGAACCGCTGGGCCGAAAGCTTTTGTCAG370.12668195980415656No Hit
ACACCCGGGCATGTCCCTGTTTATCTTTAATGTAAACCATGCACAAAACTCAGCAGACTCCTTG370.12668195980415656No Hit
ACACCCGGGGGTGCTGGAGCCACTGTCGCCGAGCTCGGGCCACGCTGCTTCTCCTCGCCAGTCG370.12668195980415656No Hit
ACACCCGGGGAACTACTTAGTCAAATGTGATACCATAAATTTAAACAGTTTACCATTTTTCAAT370.12668195980415656No Hit
ACACCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG370.12668195980415656No Hit
ACACCCGGGTGAGACCCGGGATGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAGT360.12325812305269285No Hit
ACACCCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAG360.12325812305269285No Hit
ACACCCGGGCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTC350.11983428630122916No Hit
ACACCCGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGCGGACCGCGCCGG340.11641044954976547No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC340.11641044954976547No Hit
ACACCCGGGATCAAGAAGGTGGTGAAGCAGGCATCTGAGGGCCCACTGAAGGGCATCTTGGGCT340.11641044954976547No Hit
ACACCCGGGAACACATGGCAGTTCAACTTCAAGTTCAACCTGACAGAGGATATGTACTCCCAGG330.11298661279830179No Hit
ACACCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG330.11298661279830179No Hit
ACACCCGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA320.10956277604683808No Hit
ACACCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG310.10613893929537441No Hit
ACACCCGGGATGTGCCCCGGGCAGTGTTCGTAGACCTGGAACCCACGGTCATCGATGAAGTTCG310.10613893929537441No Hit
ACACCCGGGGTGGCACGCTCACTAGTCTGGCTTTGCTGCCCACGAAACGCCACCTCCTGCTGGG300.1027151025439107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTTA351.4793841E-858.42634623
CTCCCAT258.098894E-658.42634224
AATTATC258.098894E-658.42634245
CATCAAC258.098894E-658.42634226
TGCCAGT258.098894E-658.42634224
ATACATC258.098894E-658.42634223
CTTTACC150.00450850158.4263425
ATCTATC150.00450850158.4263445
CGCAGAT150.00450850158.4263425
TCCACCC150.00450850158.4263425
TCTTTAC150.00450850158.4263424
TTTACCC150.00450850158.4263426
AAGTAGG258.1828075E-658.3252640
AAATTTG351.4997568E-858.3252639
AATTTGT351.4997568E-858.3252640
GATACAT258.1828075E-658.3252622
CACAATT258.1828075E-658.3252642
CGCCATT351.4997568E-858.3252620
GTAGGTC258.1828075E-658.3252642
ATCACAA258.1828075E-658.3252640
Produced by FastQC (version 0.11.5)