SRR1526891_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26147
Sequences flagged as poor quality	0
Sequence length	56
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	321	1.2276743029793094	No Hit
ACACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT	269	1.028798714957739	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	264	1.0096760622633572	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	227	0.8681684323249321	No Hit
ACACCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	204	0.780204229930776	No Hit
ACACCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	111	0.4245228898152752	No Hit
ACACCCGGGGGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCT	93	0.35568134011550084	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	87	0.3327341568822427	No Hit
ACACCCGGGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTATAGTA	80	0.30596244311010823	No Hit
ACACCCGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	77	0.2944888514934792	No Hit
ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	67	0.25624354610471567	No Hit
ACACCCGGGCATGTGTTTCAAAATAAGGGAACTTCAGTCACCTCAAAGGTAAAATA	67	0.25624354610471567	No Hit
ACACCCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	61	0.23329636287145752	No Hit
ACACCCGGGGAACTACTTAGTCAAATGTGATACCATAAATTTAAACAGTTTACCAT	60	0.22947183233258117	No Hit
ACACCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	60	0.22947183233258117	No Hit
ACACCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	55	0.2103491796381994	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	54	0.20652464909932305	No Hit
ACACCCGGGGTGTAACCTTTAGAATTATTTGCAGAATATCCTTTTAGCTTATATGT	52	0.19887558802157032	No Hit
ACACCCGGGACTGCTTAGATGGTGGGCCTGCCCTCACCAAACAGGAATTTCTATTA	50	0.19122652694381764	No Hit
ACACCCGGGGTGGACCTTCCTTCCTCCCATGGCATGTGCAAGTAGGTCTGCGTGAC	49	0.1874019964049413	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	47	0.17975293532718858	No Hit
ACACCCGGGTTGCCCCATCCCCAAACCTGTCCAGCACTTATGTAAGAACGCATTTG	47	0.17975293532718858	No Hit
ACACCCGGGCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATG	47	0.17975293532718858	No Hit
ACACCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	46	0.17592840478831223	No Hit
ACACCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.17210387424943588	No Hit
ACACCCGGGGGTGCCAGAGAGAGTAGAGGAGAGCAGAGAGCCGTGGAGCGACAGTG	44	0.16827934371055953	No Hit
ACACCCGGGGGTTTCCACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCC	42	0.16063028263280682	No Hit
ACACCCGGGGGCTATTACCTCATTCCTTTCACTGGGATTGTAGGATTACTGGTTTT	42	0.16063028263280682	No Hit
ACACCCGGGATGTGCCCCGGGCAGTGTTCGTAGACCTGGAACCCACGGTCATCGAT	41	0.15680575209393047	No Hit
ACACCCGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	41	0.15680575209393047	No Hit
ACACCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC	40	0.15298122155505411	No Hit
ACACCCGGGGGTGCTGGAGCCACTGTCGCCGAGCTCGGGCCACGCTGCTTCTCCTC	37	0.14150762993842506	No Hit
ACACCCGGGGAGCCGCTCCTGCGCGCTCCCGGGCTTGGAGTCCGAGCACGTTCTCG	37	0.14150762993842506	No Hit
ACACCCGGGGCTCTAAAATGGAAGTGGAGCGGACTCCTAGTACCGCTAGAAGCTGC	36	0.1376830993995487	No Hit
ACACCCGGGGATTGCTGAGGCGGCGAGGGAGAGCGTTGGGCTTACCTCACTGCTTT	32	0.12238497724404329	No Hit
ACACCCGGGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGA	32	0.12238497724404329	No Hit
ACACCCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT	32	0.12238497724404329	No Hit
ACACCCCGTCATTTTGGCCTCGATCGTGGACAGCTACGAACGCCGCAACGAGGGAG	31	0.11856044670516692	No Hit
ACACCCGGGAGCATTGCAGAGACACCAACTCCAGTAATTGTAGTCACCTCTTCCTT	31	0.11856044670516692	No Hit
ACACCCGGGCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAG	30	0.11473591616629059	No Hit
ACACCCGGGCTTGTGTCAGATGACATCATGGACTCTTCCCTCACTCGCCGGGGACA	30	0.11473591616629059	No Hit
ACACCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCG	30	0.11473591616629059	No Hit
ACACCCGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACT	29	0.11091138562741425	No Hit
ACACCCAGGGGACACCGTCGTAGAGTCGTCTGGGTTGAACCGCTGGGCCGAAAGCT	29	0.11091138562741425	No Hit
ACACCCGGGGACGAGATGGGGGAAAGCATGGCAGATGATCCACGGAATTTAAAAAA	28	0.10708685508853788	No Hit
ACACCCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGC	28	0.10708685508853788	No Hit
ACACCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA	28	0.10708685508853788	No Hit
ACACCCGGGGTGGCTTACCTCGTTCACCAAATTGGGTGCACACTCACTGCTCTTGG	28	0.10708685508853788	No Hit
ACACCCGGGAACACATGGCAGTTCAACTTCAAGTTCAACCTGACAGAGGATATGTA	27	0.10326232454966153	No Hit
ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	27	0.10326232454966153	No Hit
ACACCCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	27	0.10326232454966153	No Hit
ACACCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.10326232454966153	No Hit
ACACCCGGGGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAGTGACCT	27	0.10326232454966153	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTGA	15	0.0078337705	50.79492	49
GCGTGAC	15	0.0078337705	50.79492	50
ATTGCTT	20	3.836463E-4	50.695908	48
CAGCAAG	30	9.670639E-7	50.303677	47
CCAGCAA	30	9.670639E-7	50.303677	46
CTCACTG	15	0.008141466	50.303677	46
TCTGCGT	20	3.9869474E-4	50.303673	47
TTACCTC	15	0.008204068	50.206566	42
AGTAGGT	15	0.008204068	50.206566	41
TGCCCAG	35	4.8647053E-8	50.206566	43
CTTACCT	15	0.008204068	50.206566	41
ACCTCAC	15	0.008204068	50.206566	44
GTAGGTC	15	0.008204068	50.206566	42
TACCTCA	15	0.008204068	50.206566	43
TAGGTCT	15	0.008204068	50.206566	43
ACAGCAT	20	4.0252914E-4	50.206562	43
TGACAGC	20	4.0252914E-4	50.206562	41
AGCATTG	20	4.0252914E-4	50.206562	45
AAGTAGG	15	0.008267027	50.10983	40
TGCACCG	15	0.008267027	50.10983	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content